1000 pg/ml of recombinant human G-CSF (rhG-CSF, Chugai. Pharmaceutical Co. Ltd, Tokyo, Japan) in 100 ml of PBS containing 0·1% deionized crystallized ...
British Journal of Haematology, 1999, 105, 486–490
SHORT REPORT
Serum granulocyte colony-stimulating factor levels in patients with chronic neutropenia of childhood: modulation of G-CSF levels by myeloid precursor cell mass M A SAO K OBAYA SHI , 1 K AZU H IRO U EDA , 2 S EIJI K O J I M A , 3 H I RO K A ZU N ISHI HIRA , 4 A K I R A I S H I G U RO , 5 T O S H I K A Z U S H IM B O 5 A ND TATS U TO S H I N AK A HATA 6 1Department of Children’s Health, Faculty of Education, Hiroshima University, Hiroshima, 2Department of Paediatrics, Hiroshima University School of Medicine, Hiroshima, 3 Division of Haematology/Oncology, Children’s Medical Centre, Japanese Red Cross Nagoya First Hospital, Nagoya, 4 Division of Oncology, Kanagawa Children’s Medical Centre, Yokohama, 5Department of Paediatrics, Mizonokuchi Hospital, Teikyo University School of Medicine, Kawasaki, and 6Department of Clinical Oncology, Institute of Medical Science, University of Tokyo, Tokyo, Japan Received 18 January 1999; accepted for publication 3 February 1999
Summary. The serum G-CSF levels of eight patients with severe congenital neutropenia (SCN) were found to be significantly higher than those of 22 patients with chronic benign neutropenia (CBN). The relative number of cells expressing the G-CSF receptor in light density bone marrow cells (LDBMC) was lower in patients with SCN than in patients with CBN or in normal subjects. When recombinant human G-CSF was incubated with LDBMC, G-CSF levels were decreased by LDBMC from normal subjects and CBN
patients, but not by those from SCN patients. Serum G-CSF concentrations, which are affected by mature neutrophils, may also be modulated by myeloid precursor cells in the bone marrow.
Granulocyte colony-stimulating factor (G-CSF) is thought to be the main physiological regulator of granulopoiesis. Serum concentration of G-CSF is primarily related to the absolute neutrophil count (ANC) in peripheral blood. In some clinical situations low neutrophil counts coexist with high serum G-CSF levels (Kojima et al, 1996; Mempel et al, 1991; Watari et al, 1989). Chronic neutropenia in childhood is defined as an abnormally low ANC of
