Sep 17, 2012 - Set2-mediated methylation of histone H3 at Lys 36 (H3K36me) is a co-transcriptional event that is necessary for the activation of the.
LETTER
doi:10.1038/nature11326
Set2 methylation of histone H3 lysine 36 suppresses histone exchange on transcribed genes Swaminathan Venkatesh1, Michaela Smolle1, Hua Li1, Madelaine M. Gogol1, Malika Saint2, Shambhu Kumar2, Krishnamurthy Natarajan2 & Jerry L. Workman1
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in set2D cells (Fig. 1c) and was dependent on gene length (Fig. 1d). Thus, set2D causes increased histone exchange and accumulation of H3K56ac over ORFs. Loss of Set2-mediated H3K36me also increases H4 acetylation (H4ac) across ORFs1,3. To determine whether increased exchange correlated with increased acetylation, we examined the distribution of H4ac (normalized to Myc–H3) in the wild-type and set2D exchange strains (Supplementary Fig. 3a). In set2D cells, H4ac increased towards the 39 end of ORFs over the wild type (Supplementary Fig. 3b) and was positively correlated with gene length (Supplementary Fig. 3c). We selected RNAPII-regulated genes that showed increased H4ac over a
