Simultaneous Determination of Enalapril and Hydrochlorothiazide in

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Apr 25, 2014 - Department of Medicinal Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura 35516, Egypt. *Author to ... (ENM) and hydrochlorothiazide (HCT) in their pharmaceutical pre- ..... Wilson and Gisvold's Textbook of.

Journal of Chromatographic Science Advance Access published April 25, 2014 Journal of Chromatographic Science 2014;1– 7 doi:10.1093/chromsci/bmu024

Article

Simultaneous Determination of Enalapril and Hydrochlorothiazide in Pharmaceutical Preparations Using Microemulsion Liquid Chromatography Mohammed E. A. Hammouda*, Mohamed A. Abu El-Enin, Dina T. El-Sherbiny, Dalia R. El-Wasseef and Saadia M. El-Ashry Department of Medicinal Chemistry, Faculty of Pharmacy, University of Mansoura, Mansoura 35516, Egypt *Author to whom correspondence should be addressed. Email: [email protected] Received 9 April 2013; revised 10 March 2014

A rapid high-performance liquid chromatography procedure for analytical quality control of mixture containing enalapril maleate (ENM) and hydrochlorothiazide (HCT) in their pharmaceutical preparations was developed using a microemulsion as an eluent. The separation was performed on a column packed with cyano-bonded stationary phase adopting UV detection at 210 nm using a flow rate of 1 mL/min. The optimized microemulsion mobile phase consisted of 0.2 M sodium dodecyl sulfate, 1% octanol, 10% n-propanol and 0.3% triethylamine in 0.02 M phosphoric acid, and pH was adjusted at 3.5. The proposed method was found to be linear over the concentration ranges 1 – 100 and 0.05 – 5 mg/mL for ENM and HCT, respectively with a correlation coefficient of 0.9999 for both drugs. The developed method was validated in terms of specificity, linearity, lower limit of quantification, lower limit of detection, precision and accuracy. The proposed method is rapid (5 min), reproducible (relative standard deviation

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