Innovare Academic Sciences
International Journal of Pharmacy and Pharmaceutical Sciences ISSN- 0975-1491
Vol 7, Issue 11, 2015
Original Article
SIMULTANEOUS SPECTROPHOTOMETRIC ESTIMATION OF MOXIFLOXACIN HYDROCHLORIDE AND DOXORUBICIN HYDROCHLORIDE NAYAN PRADHAN*, HIYASHREE RAJKHOWA, HEMANT GIRI, BHUPENDRA SHRESTHA Department of Pharmaceutical Analysis and Quality Assurance, Himalayan Pharmacy Institute, Majhitar 737132, East Sikkim, India Email:
[email protected] Received: 12 Dec 2014 Revised and Accepted: 10 Sep 2015 ABSTRACT Objective: To develop a simple, accurate, and precise spectrophotometric method for the simultaneous estimation of moxifloxacin hydrochloride (MOX) and doxorubicin hydrochloride (DXR).
Methods: MOX and DXR solution were simultaneously determined in 0.1M HCl at their respective λmax. The absorbance λmax of MOX and DXR was 295 nm and 480 nm, respectively. The developed method was validated according to ICH guidelines for parameters like linearity, accuracy, precision, ruggedness and robustness.
Results: Molar absorptivities of MOX and DXR were found to be more in 0.1M HCl with compared to water, methanol and 0.1M NaOH. Linearity was obtained over the range 0.5–20.0 μg/ml and 1.5-50 μg/ml with a lower limit of quantitation of 0.25 μg/ml and 1.5 μg/ml for MOX and DXR, respectively. For each level of samples, inter-and intra-day precision ( % RSD) was
