TRAP and ALP activities in saliva and GCF (n=9) with and without the addition of PI were assayed at 0 ..... bonate buffer (pH 9.8), and were incubated at 30°C.
Arch. Biol. Sci., Belgrade, 65 (3), 1131-1140, 2013
DOI:10.2298/ABS1303131K
STABILITY OF LACTATE DEHYDROGENASE, ASPARTATE AMINOTRANSFERASE, ALKALINE PHOSPHATASE AND TARTRATE RESISTANT ACID PHOSPHATASE IN HUMAN SALIVA AND GINGIVAL CREVICULAR FLUID IN THE PRESENCE OF PROTEASE INHIBITOR NURFATHIHA ABU KASIM1, SHAHRUL HISHAM ZAINAL ARIFFIN1, MUHAMMAD ASHRAF SHAHIDAN1, INTAN ZARINA ZAINOL ABIDIN1, SAHIDAN SENAFI1, ABDUL AZIZ JEMAIN3 and ROHAYA MEGAT ABDUL WAHAB2* School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia 2 Department of Orthodontics, Faculty of Dentistry, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia 3 DELTA, School of Mathematics, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 Bangi, Malaysia 1
Abstract - The stability of aspartate aminotransferase (AST), lactate dehydrogenase (LDH), tartrate resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) activities from saliva and gingival crevicular fluid (GCF) with and without the addition of protease inhibitor (PI) at room temperature (RT; 25⁰C), 4°C and -20°C were investigated. AST, LDH, TRAP and ALP activities in saliva and GCF (n=9) with and without the addition of PI were assayed at 0 (control), 12, 24, 48, 72 h, one and two weeks. A paired t-test showed there were a significant differences (p
