Study on the effect of chronic administration of “Chandraprabha Bati” on different hematologic parameters in male Sprague-Dawley rats Manoth Biswas1, Md Mamun Shikdar1, Sayeman Islam Niloy2, Nelufar Yasmen2, Md.Sabbir Ahmed2, Afsana Tajmim2, Mohammad Jashim Uddin2* and MSK Choudhuri1 1
2
Department of Pharmacy, Jahangirnagar University, Savar -1342, Dhaka, Bangladesh. Department of Pharmacy, Jessore University of Science and Technology, Jessore-7408, Bangladesh. *Corresponding Author:
[email protected]
Background and objectives: Chandraprabha Bati (CPB)an Ayurvedic preparation is used in the traditional medicine in the treatment of dysuria in the rural population. To find out the toxicological characteristic of CPB to have a understanding of its hematological safety profile.
Methods: Male Sprague-Dawley rats chronically administered for 28 days at dose of 400 mg/kg to note its effect on (i) TC & DC (ii) Rbc count and relevant parameters, (iii) Platelet count and parameters, and (iv) ESR, bleeding and clotting time.
Results: Control
CPBm
Increase/Decrease (%)
p value
WBC
3.7375±0.39185
4.1500± 0.22281
Incr 11.03%
0.376
Lymphocyte Monocyte Monocyte(%)
2.6162± 0.30072 0.0638± 0.00905 1.7500± 0.16366
3.0113± 0.16706 0.0550± 0.00627 1.3750± 0.18298
Incr 15.10% Decr13.79% Decr 21.42%
0.270 0.440 0.149
6
WBC
9
thousand / mcL
thousand / mcL
Parameters
7 5 3 WBC
CON
CPBm
3.7375
4.15
Lymphocyte
5 4 3 2 Lymphocyte
CON
CPBm
2.6162
3.0113
Monocyte
2
0.4 percentage
thousand / mcL
0.5
0.3 0.2 0.1 0 Monocyte
CON
CPBm
0.0638
0.055
Monocyte (%)
1.5 1 0.5 0 Monocyte (%)
CON
CPBm
1.75
1.375
There is an [11.04 %] increase in the number of white blood cell count of the male rat, the increase though not significant yet it was prominent (p=0.376). There is an [15.10 %] increase in the absolute count of Lymphocytes of the male rat, the increase though not significant yet it was prominent (p=0.270). There is a [13.79 %] decrease in the absolute count of Monocytes of the male rat, the decrease though not significant yet it was prominent (p=0.440). There is a [21.43 %] decrease in the percentage of Monocyte count of the male rat, the decrease though not significant yet it was prominent (p=0.149).
Conclusion: Chronic administration of Chandraprabha Bati (CPB) may cause decrease in wbc count, monocytopenia and lymphopenia.
Keywords: Ayurvedic medicine, Chandraprabha Bati, Sprague-Dawley rat, Hematology, WBC, TC, DC.
