Subcellular Biochemical Changes in the Jejunal Mucosa of Dogs with

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enteropathy in the dog is presented that could help achieve a ... showed a marked loss of alkaline phosphatase ... bembrane proteins are a consequence of,. b r relate to the ... of parenteral feeding on bile acid (BA) Lindbile lipid metabolism.
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Medical Research Society k i l l i n g of Staph.aureus was s t u d i e d i n p e r i p h e r a l blood m n o c y t e s by a m o d i f i c a t i o n of t h e 'forced phagocytosis' method ( T e r r i t o M.C. and C l i n e M . J . J.Immunol., 1977, 187-192) i n 10 p a t i e n t s w i t h PBC, 9 w i t h CAH and c o n t r o l s . Monocyte bacterial k i l l i n g w a s normal i n b o t h d i s e a s e s . W e conclude that t h e i n c r e a s e d s u s c e p t i b i l i t y t o i n f e c t i o n i n p a t i e n t s with p l c o h o l i c c i r r h o s i s may be r e l a t e d t o mpairment of n e u t r o p h i l phagocyte f u n c t i o n . he i n - v i t r o observations i n p a t i e n t s w i t h PBC suggest t h a t a t t e n t i o n should be paid t o t h e o s s i b i l i t y of i n c r e a s e d s u s c e p t i b i l i t y t o acterial i n f e c t i o n i n some forms of nonalcoholic l i v e r disease.

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150 SWCELLXJIAR BIOCHEMICAL CHANGES THE JEJUNAL MUCOSA OF DOGS WITH NATURALLY OCCURRING BACTERIAL OVERGROWTH R.M. BATl', M.W. CARTER AND T . J . PETERS Department o f V e t e r i n a r y P a t h o l o g y , U n i v e r s i t y of L i v e r p o o l L69 3BX and D i v i s i o n of C l i n i c a l C e l l B i o l o g y , MRC C l i n i c a l R e s e a r c h C e n t r e , H a r r o w HA1 3UJ

Many anatomical and f u n c t i o n a l d i s o r d e r s may be associated w i t h b a c t e r i a l o v e r g r o w t h i n t h e m a l l i n t e s t i n e but t h e r o l e of b a c t e r i a in t h e p a t h o g e n e s i s of t h e mucosal changes i s not c l e a r l y defined. A naturally occurring enteropathy in t h e dog i s presented t h a t could help achieve a b e t t e r understanding o f t h e i n t e r - r e l a t i o n e h i p s b e t w e e n an sbnonual f l o r a and mucosal damage. A f f e c t e d a n i m a l s w e r e diagnosed a s described previously ( B a t t & M o r g a n , 1 9 8 2 , 32: 17) and c o m p r i s e d e i g h t !Res.Vet.Sci. G e n n a n Shepherd dogs w i t h n o r m a l ancreatic k c t i o n , elevated serum o l a t e and reduced serum vitamin B12 levels. B a c t e r i a l o v e r g r o w t h was Iconfirmed i n s i x d o g s by a e r o b i c and kinaerobic c u l t u r e o f duodenal j u i c e ~ ( 1 0 5 1 0 7 orgs./ml; c o n t r o l < 1.0~). Barium s t u d i e s demonstrated no anatomical a b n o r m a l i t i e s and jejunal b i o p s i e s hevealed m i n i m a l h i s t o l o g i c a l changes. Isopycnic d e n s i t y gradient centriW g a t i o n of j e j u n a l b i o p s i e s ( B a t t & P e t e r s , 1978, R e s . V e t . S c i . 94) s h o w e d a marked loss of a l k a l i n e phosphatase a c t i v i t y from t h e brush border. In c o n t r a s t , Y - g l u t a m y l transIferase a c t i v i t y w a s enhanced in brush border fragments of s l i g h t l y increased podal density but there w e r e no changes .in t h e a c t i v i t i e s of d i s a c c h a r i d a s e s o r leucyl-8-naphthylamidase. The a c t i v i t y of N-acetyl-B-glucosamiidase w a s J i n c r e a a e d i n t h e soluble f r a c t i o n s , rindicative of enhanced lysosome1

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K r a g i l i t y , but other organelles were unaltered. Future s t u d i e s w i l l e x a m i n e whether these s p e c i f i c a l t e r a t i o n s i n m i c r o v i l l u s bembrane p r o t e i n s a r e a consequence o f , b r r e l a t e t o t h e d e r i v a t i o n of t h e pvergrowth.

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ACID AND BILE LIPID METABOLISM DURING TOTAL PARENTERAL NUTRITION (TPN) I N THE i?.&'l

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S . k j o , G.M. Murphy and R . H . 5 Gastroenteroloey Unit, Guy's H o s p i t a l rind Medical School, London SEl 9RT

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Abnormal l i v e r f m c t i o n . c h d e s t a s i : ; , I i t h o g e n i c h i l e and g a l l s t o n e s may all c.omp!icnte TPN but surpri6inF;ly l i t t l e i s known about tho el'fectt; of p a r e n t e r a l feeding on b i l e a c i d (BA) Lindbile l i p i d metabolism. Therefore, i n a c u t e h i l e f i s t u l a r a t s , w e used t h e E r i k s s o n w:inhout tec:wique t o measure t h e BA-dependent m d -independent f r a c t i o n s of b i l e flow, BA c o n c e n t r a t i o n , secret -ion and s y n t h e s i s r a t e s , pool size and anatonical 1ocali:;ation and b i l e 1 i pid o u t p u t , r e l a t inE changes t o l i v e r h i s t o l o g y end f u n c t i o n a f t e r 7 days TPN (n.7) 71' Qra! f e e d i n c + I V catheters and harnessen ( c o n t r o l s ; n = 6 ) . Results: B i l e flow diminished s i g n i f i c a n t l y from hi6 t o h20 a f t e r f i s t u l - r t i o n b u t t h e mean BA-dependent f r a c t i m of S:le f:ov incin contr9:s t o 576 daring TPN. reased from SA c o n c e n t r a t i o n increased significnnt1:r over t h e f i r s t 14h of h i l i a r y drainage and, al.thout;h BA s e c r e t i o n and s y n t h e s i s r;tc did not, chmp, pool s i z e (po1/100c BW) increased from 4 3 + ~ ~ 3.6 i.n c o n t r o l . - t o 62.326.2 during TPN ($0.05). In n o n - f i s t u l a rats, f r e s h w r t l i v e r weight (C/lOOg BW) increased from 3.45b.58 :n fa:;ting c o n t r o l s t o 5.0229.84 d u r i n g TPN (p