n=17. All values shown are mean+s.d. P values were calculated by unpaired t-test. ... The fraction of fosGFP+ neurons within a given field did not change ... collected using custom software designed for Igor Pro (Wavemetrics, Lake Oswego,.
Neuron, Volume 68
Supplemental Information An Embedded Subnetwork of Highly Active Neurons in the Neocortex Lina Yassin, Brett L. Benedetti, Jean-Sébastien Jouhanneau, Jing A. Wen, James F.A. Poulet, and Alison L. Barth
Inventory of Supplemental Information Figure S1. Quantification of fos-IR and fosGFP expression, and distribution of firing rates in wild-type and fosGFP layer 2/3 pyramidal neurons. Figure S2. FosGFP+ neurons are less excitable by current-evoked firing. Table S1. Electrophysiological properties of fosGFP+ neurons. Supplemental Experimental Procedures Supplemental References
Figure S1
Figure S1. Quantification of fos-IR and fosGFP expression, and distribution of firing rates in wild-type and fosGFP layer 2/3 pyramidal neurons. (A) Percentage of fos-IR (red) and fosGFP (green) in layer 2/3 across cortical regions (motor cortex (M1), somatosensory cortex (S1) and visual cortex (V1). (B) Example of photo image of recorded cells, in layer 2/3 (left), showing the pyramidal nature of the cells (middle) containing spines (left), scale bars are 200, 20, 5 m respectively. (C) Mean spontaneous firing rate of wild-type, fosGFP- and fosGFP+ neurons collected during single-cell recording (non-pairs, see text). (D-I) Sample traces of spontaneous activity in wild-type (wt) (D) fosGFP- (F), and fosGFP+ (H) neurons and corresponding histograms for firing spontaneous firing rates across the population of recorded cells (E, G, I respectively).
Figure S2
Figure S2. FosGFP+ neurons are less excitable by current-evoked firing. (A-C) Example traces of responses elicited by current injection of 20, 60 and 120 pA for a fosGFP- (black) and a fosGFP+ (green) neuron. (D) The minimal current required to elicit one action potential (rheobase current) is significantly elevated in fosGFP+ neurons. N’s are as indicated on bar graph. (E) Firing frequency versus current injection (F-I
curve) shows a suppressed input-output function for fosGFP+ compared to fosGFPneurons (n=9 for both groups). *p
