center of gravity of the animal, its nose, and even the end or the beginning of the tail. ... manually, using an ethological piano (labeled keyboard with labeled key that .... may fail to properly fit the model and some confusion or fall-off may follow.
Nature Methods
Computerized video analysis of social interactions in mice Fabrice de Chaumont, Renata Dos-Santos Coura, Pierre Serreau, Arnaud Cressant, Jonathan Chabout, Sylvie Granon & Jean-Christophe Olivo-Marin
Supplementary Figure 1
Chronogram representing all the β2−/− and C57BL/6J experiments, sorted by events and mouse strains
Supplementary Figure 2
Graphical user interface of the MiceProfiler tracking module
Supplementary Figure 3
Graphical user interface of the MiceProfiler chronogram module
Supplementary Figure 4
Graphical user interface of the MiceProfiler temporal analysis module
Supplementary Figure 5
Quantification of manual supervisions during automatic tracking using MiceProfiler
Supplementary Figure 6
Comparison of social behaviors between C57BL/6J, 129SV and DBA2 mouse strains
Supplementary Note 1
Tracking
Supplementary Note 2
Comparison between MiceProfiler and existing software
Supplementary Note 3
Limitations, sources of errors and future improvements
Supplementary Note 4
Further thoughts: Ethical research
Note: Supplementary Software and Supplementary Video 1 are available on the Nature Methods website.
Nature Methods: doi:10.1038/nmeth.1924
Supplementary Figure 1 Chronogram representing all the β2‐/‐ and C57BL/6J experiments, sorted by events and mouse strains.
Nature Methods: doi:10.1038/nmeth.1924
1
Supplementary Figure 2 Graphical user interface of the MiceProfiler tracking module
Views of the tracking interface within MiceProfiler (see Supplementary Software and http://icy.bioimageanalysis.org). The left hand side of the image is the parameter interface and the right hand side window is a viewer. The user can zoom in the image to control the tracking, and also possibly correct the current tracking by simply dragging the head and bottom control.
Nature Methods: doi:10.1038/nmeth.1924
2
Supplementary Figure 3 Graphical user interface of the MiceProfiler chronogram module
Screenshot of the second module: On the left hand side is the scrollable window chronogram of the mouse. Each line represents an event. On the right hand side are two sub windows: in the top are the parameters for the distance and speed thresholding, some parameters about the video, and the time window of the computation. The bottom view represents the video at the current time point (the vertical line on the left hand side area). The video is overlaid with the tracking of the two mice and the lines corresponding to the front of the animal and 60°and 120° by each side, which correspond to the field of view.
Nature Methods: doi:10.1038/nmeth.1924
3
Supplementary Figure 4 Graphical user interface of the MiceProfiler temporal analysis module
Screenshot of the temporal analysis plugin in MiceProfiler
Nature Methods: doi:10.1038/nmeth.1924
4
Supplementary Figure 5 Quantification of manual supervisions during automatic tracking using MiceProfiler
Number of manual supervisions needed per strain and animal during the automated tracking.
Nature Methods: doi:10.1038/nmeth.1924
5
Supplementary Figure 6 Comparison of social behaviors between C57BL/6J, 129SV and DBA2 mouse strains
The number (a) and duration (b) of social contact are different between the three strains (genotype effect respectively: F (2,17) =9.3, p=0.002 and F (2,17) =5.8, p=0.01). Only social duration contact evolved significantly with time (F (2,17) =5.0, p=0.04) while the number of social contact remained stable (F
