Phagocytosis of TDM-labeled beads was not altered in. BMMs from WT ... iBMM cells were transfected separately with Flag-CD11b, Flag-Syk, V5-Mincle, HA-.
Supplementary Figure Legends
Supplementary Figure 1. Phagocytosis of TDM-labeled beads was not altered in BMMs from WT, CD11b-/-, or Mincle-/- mice. Macrophages from WT, CD11b-/-, and Mincle-/- mice were plated overnight, and GFPlabeled, uncoated or TDM-coated latex beads were added to the culture. Cells were collected at the indicated times, and internalization of the beads was analyzed by flow cytometry. Data are representative of three independent experiments.
Supplementary Figure 2. Enhanced proinflammatory gene expression in CD11b-/BMMs upon TDM stimulation. Relative mRNA expression of proinflammatory genes was quantified in WT and CD11b-/- BMMs treated with TDM (50 mg/ml) for the indicated times. Data are representative of three independent experiments. *P
