Dicoumarol. ISR. E-luc e xp re ssio n. (in d u ctio n s folds). None. ssRNA. D. 0. 20. 40. 60. 80. 100. 120. 140. U. C. G. A. DMSO. Brequinar 0.1 µM. Brequinar 1 ...
A
120
T = 48 h
MV-Luc replication (% of DMSO control)
100 80
**
60
**
40 20
**
0 0
15 5
30 10
45 15
60 20
200 180 160 140 120 100 80 60 40 20 0
T = 72 h
ns
ns
0
15 5
DD363 (µM)
B
**
**
**
350 Living cells (arbritary units)
45 15
60 20
DD363 (µM)
*
400
30 10
300
0h T0 24h 48h 72h
250 200 150 100 50 0 00
2,5 7.5
5 15
10 30
20 60
DD363 (µM) FIG. S1. Kinetic study of DD363 effects on MV growth and cellular proliferation. (A) HEK-293T cells were infected with MV-Luc (MOI = 0.1), and incubated with increasing concentrations of DD363 or DMSO alone. After 48 or 72 h of culture, luciferase activity was measured to quantify viral growth. Results are expressed as a percentage of luminescence signals relative to DMSO control (means ± SD of 5 and 3 independent experiments, respectively). ** P
