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Jun 2, 1989 - 3 slices rye-wheat bread, butter, Nutella,. 2 apricots. 1 tall cup of coffee with whole milk, 1 glass of ACE-spritzer. S1. 2 glasses of water lunch.
Metabolites 2016, 6, 18; doi: 10.3390/metabo6020018

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Supplementary Materials: Detection of Volatile Metabolites of Garlic in Human Breast Milk Laura Scheffler, Yvonne Sauermann, Gina Zeh, Katharina Hauf, Anja Heinlein, Constanze Sharapa and Andrea Buettner

Figure S1. Cont.

Metabolites 2016, 6, 18; doi: 10.3390/metabo6020018

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Figure S1. Aroma profiles of human milk samples of test persons (a–f). Table S1. Dietary record-test person a. Day of Dietary Record

Day 1

Day 2

Sampling Day

Type of Meal

Food

Beverages

break-fast S1 lunch S2 dinner S3 break-fast S1 lunch S2 dinner S3 break-fast S1 lunch S2 dinner S3

cereal apple pasta with cheese sauce apple, banana bread with cheese

coffee fennel-anis-caraway-tea coffee, water

cereal apple semolina porridge with berries apple, chocolate cake bread, pretzel with cheese, aspic

coffee, fennel-anis-caraway-tea

croissant, apple, banana

S1, S2, S3: Snacks.

water

coffee, water water

Metabolites 2016, 6, 18; doi: 10.3390/metabo6020018

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Table S2. Dietary record-test person b. Day of Dietary Record

Type of Meal break-fast S1

Day 1

lunch S2 dinner S3 break-fast S1 lunch

Day 2

S2 dinner S3 break-fast

Sampling Day

S1 lunch S2 dinner S3

Food cereal with fruits, amaranth and milk, α-Thyroxin 50 mg

Beverages cappuccino

water tofu-stir-fried vegetables with rice and curry water sauce chocolate croissant cappuccino sugared pancake with raisins with apple sauce water ½ banana water cereal with fruits, amaranth and milk, cappuccino α-Thyroxin 50 mg 1 banana water mozzarella-tomato with balsamic vinegar and water pretzel ½ Bavarian doughnut cappuccino pasta with soy Bolognese sauce and salad water (cucumber, tomato, carrot, sunflower seeds, pumpkin seeds) water whole-grain bread with quark, currant-jelly, cacao honey and butter turkish spinach cake, endive salad with carrots

water

S1, S2, S3: Snacks. Table S3. Dietary record-test person c. Day of Dietary Record

Day 1

Day 2

Sampling Day

Type of Meal Food Beverages break-fast cheese roll coffee S1 lunch roll with chicken, salad and sauce (without garlic) thyme tea, water S2 dinner pasta with tomato sauce (mushrooms, tomatoes) water, apple juice S3 chocolate break-fast cereal (grains, apple, milk) coffee S1 lunch cake with nuts, roll with herbs thyme tea, water S2 rice pudding with cherries coconut milk water dinner pasta with tomato sauce (mushrooms, tomatoes) S3 potato chips break-fast cereal (grains, apple, milk) coffee, water S1 lunch cheese roll S2 chocolate dinner S3 S1, S2, S3: Snacks.

Metabolites 2016, 6, 18; doi: 10.3390/metabo6020018

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Table S4. Dietary record-test person d.

Day of Dietary Type of Meal Food Record break-fast cereal with wild berries S1 lunch bread and ham Day 1 S2 chocolate dinner gratin S3 break-fast cereal with wild berries S1 lunch gratin Day 2 S2 dinner gratin, mandarins S3 cookies break-fast cereal with wild berries S1 lunch gratin: zucchini, tomato, feta cheese, egg Sampling Day S2 chocolate dinner S3 S1, S2, S3: Snacks.

Beverages blood orange juice

nursing tea blood orange juice

oriental, red tea good-night tea blood orange juice

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Table S5. Dietary record-test person e.

Day of Dietary Record

Type of Meal

Food

Beverages

break-fast

3 slices rye-wheat bread, butter, Nutella, 2 apricots

1 tall cup of coffee with whole milk, 1 glass of ACE-spritzer

S1 Day 1

lunch S2 dinner

2 glasses of water polenta with stir-fried vegetables (zucchini, pepper, kohlrabi) carrot, nectarine and banana 5 slices rye-wheat bread (1x with butter, 2x with liverwurst, 2x with cheese

S3 break-fast

Day 2

S1 lunch S2 dinner S3 break-fast S1

Sampling Day

lunch S2 dinner S3

1 glass of water 2 glasses of water 1 glass of water 2 glasses of water, 1 glass of currant spritzer

3 slices rye-wheat bread, 1x liverwurst, 2x 1 tall cup of coffee with whole milk, 1 glass of currant spritzer butter and marmalade, ½ apricot, ½ nectarine 1 glass of water, 1 glass of currant spritzer 3 slices rye-wheat bread with cheese, carrot 1 glass of water nectarine, apricot, Hanuta 2 glasses of water whole-grain pasta with minced meat sauce 1 glass of water (beef mince, tomato, pepper, peas), cheese Hanuta, 2 Kinder-Schoko-Bons 1 glass of water, 1 glass of tonic 3 slices rye-wheat bread with Nutella, 1 1 tall cup of coffee with whole milk, 2 glasses of water apricot, ½ nectarine 2 glasses of water, 1 glass of apple spritzer whole-grain pasta with minced meat sauce 1 glass of water (beef mince, tomato, pepper, peas), cheese Hanuta 1 small cup of coffee with milk, 1 glass of water

S1, S2, S3: Snacks.

Metabolites 2016, 6, 18; doi: 10.3390/metabo6020018

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Table S6. Dietary record- test person f.

Day of Dietary Type of Meal Record break-fast S1 Day 1

lunch S2 dinner S3 break-fast S1

Day 2

Sampling Day

lunch S2 dinner S3 break-fast S1 lunch S2 dinner S3

Food

Beverages

3 slices rye-wheat bread

coffee with soy milk

noodles with olive-tomato sauce (tomato purée, olives, basil, provençal herbs, olive oil, pizza soft cheese) trail mix, gummi bears hash browns with apple sauce Alnatura breakfast puree with rice milk

nursing tea (fenugreek, fennel, anise, caraway, lemon verbena)

eggplants with tomato sauce (according to the restaurant without onions and garlic, but with broth from Maggi) bread with margarine and ham, cucumber dark chocolate vegan marble cake bread bun with butter, ham, tomato, cucumber

S1, S2, S3: Snacks.

coffee with soy milk, Fanta water water

Metabolites 2016, 6, 18; doi: 10.3390/metabo6020018

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Table S7. Time of sampling, garlic consumption and the respective amounts of the gathered samples.

Subject test person a

test person b

test person c

test person d

test person e

test person f

Event

Time

Amount of Human Milk (g)

1st sample garlic consumption 2nd sample 3rd sample 1st sample garlic consumption 2nd sample 3rd sample 1st sample garlic consumption 2nd sample 3rd sample 1st sample garlic consumption 2nd sample 3rd sample 1st sample garlic consumption 2nd sample 3rd sample 1st sample garlic consumption 2nd sample 3rd sample

9:00 9:10 11:10 13:30 9:20 9:25 11:45 14:30 9:05 09:10 12:10 14:00 9:20 9:25 12:10 14:20 9:40 9:55 12:35 14:15 9:35 9:45 12:45 14:50

437,953 118,489 490,093 337,798 277,028 154,362 159,959 185,397 125,154 152,956 131,801 261,274 436,317 446,195 231,551 326,611 95,967 112,297