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Organic carbon speciation using thermal optical analyzer (TOA) for different ... thermal desorption and pyrolytic evolution of carbonaceous species within TOA ...

Supplementary Materials Table S1. GC–MS quantified PAHs with corresponding MS ions and calibration standards. Group

Compound

Formula

PAHs

phenanthrene anthracene methyl-phenanthrene/anthracene (A–E) fluoranthene pyrene methyl-fluoranthene/pyrene (A–G) benzo[a]anthracene chrysene benzo[b]fluoranthene benzo[a]pyrene 9-fluorenone xanthone anthrone (A–E) 2-hydroxybiphenyl 3-hydroxybiphenyl 4-hydroxybiphenyl 2-hydroxy-9-fluorenone 9-phenanthrol 1-hydroxypyrene 1-nitropyrene

C14H10 C14H10

Quantification Ion m/z 178 178

C15H12

192

191

165

2-methylanthracene

C16H10 C16H10

202 202

106 174

92 101

fluoranthene pyrene

C17H12

216

215

190

1-methylpyrene

C18H12 C18H12 C20H12 C20H12 C13H8O C13H8O2 C14H10O C12H10O C12H10O C12H10O C13H8O2 C14H10O C16H10O C16H9NO2

228 228 252 252 180 196 194 242 242 242 268 266 290 247

114 114 126 126 152 168 165 227 227 227 195 251 275 231

101 101 113 113 126 139 139 211 211 211

chrysene chrysene benzo[b]fluoranthene benzo[a]pyrene 9-fluorenone xanthone anthrone 2-hydroxybiphenyl 4-hydroxybiphenyl 4-hydroxybiphenyl 2-hydroxy-9-fluorenone 9-phenanthrol 1-hydroxypyrene 1-nitropyrene

Oxy-PAHs

Hydroxy-PAHs

Nitro-PAHs

Confirmation Ions m/z 152 89 152 89

235 250 215

Calibration Standard phenanthrene anthracene

Table S2. GC-MS quantified alkanes with corresponding MS ions and calibration standards. Alkanes

Formula

tetradecane pentadecane hexadecane heptadecane octadecane nonadecane eicosane henicosane docosane tricosane tetracosane pentacosane hexacosane heptacosane octacosane

C14H30 C15H32 C16H34 C17H36 C18H38 C19H40 C20H42 C21H44 C22H46 C23H48 C24H50 C25H52 C26H54 C27H56 C28H58

Quantification Ion m/z 57 57 57 57 57 57 57 57 57 57 57 57 57 57 57

Confirmation Ions m/z 85 198 85 212 85 226 85 240 85 254 85 268 85 282 85 296 85 310 85 324 85 338 85 352 85 366 85 380 85 394

Calibration Standard tetradecane tetradecane tetradecane tetradecane tetradecane tetradecane eicosane eicosane eicosane eicosane eicosane eicosane eicosane eicosane eicosane

Figure S1. The amount of volatile/semi-volatile compounds extracted decreased according to polarity of the solvents. Organic carbon speciation using thermal optical analyzer (TOA) for different polarity solvent fractions obtained by DEP extraction shown as (A) overlaid thermograms and (B) processed data (ratios of peak areas converted to µg). The temperatures shown are the steps used for thermal desorption and pyrolytic evolution of carbonaceous species within TOA analysis. The results are based on EOM from 10 mg of the original DEP. The figure has previously been published elsewhere [16].

100 AUC (Normalized calcium level)

Normalized calcium level

2.0

1.8

1.6

1.4

1.2

45

40

35

30

25

20

15

10

5

0

1.0

DCM 5 µg/mL DCM 2APB

80

60

40

20

*

0 - 45 min

Time (min) Figure S2. Effect of 2-APB on [Ca2+]i triggered by DCM-EOM. Cells were incubated in buffer with or without the STIM1/TRPC inhibitor 2-APB (50 µM) 30 min prior to exposure. Three minutes after measurements were started, the cells were exposed to DCM-EOM at concentrations corresponding to 5 µg/mL of the original DEP or vehicle control (DMSO). [Ca2+]i levels measured by normalized ratio of the Fura2-AM probe during exposure is presented as graph and the area under the curve (AUC) 0–45 min, as mean and mean ± SEM (n = 3). * Significantly different from no inhibitor.

Figure S3. Basic expression of calcium conductance channels in HMEC-1. Unexposed cells were harvested, mRNA isolated and gene expression measured with qPCR (n = 1). Expression is relative to the average of all seven genes.