Variations of fluorescence intensity of HNA (10 µM) at 513 nm in aqueous solution with. (bottom) ... Job's plots according to the method for continuous variations.
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Supplementary Materials: Synthesis and Application of an Aldazine-Based Fluorescence Chemosensor for the Sequential Detection of Cu2+ and Biological Thiols in Aqueous Solution and Living Cells Hongmin Jia 1, Ming Yang 1, Qingtao Meng 1,*, Guangjie He 2, Yue Wang 1, Zhizhi Hu 1, Run Zhang 3 and Zhiqiang Zhang 1,*
Figure S1. Fluorescence intensity of HNA (10 μM) at different time in DMF-HEPES buffer (20 mM, pH = 7.4, 3:7 v/v). The intensities were recorded at 513 nm, excitation at 411 nm.
Figure S2. Variations of fluorescence intensity of HNA (10 μM) at 513 nm in aqueous solution with (bottom) and without (up) Cu2+ (0–20 μM) as a function of pH. Excitation at 411 nm.
Sensors 2016, 16, 79; doi:10.3390/s16010079
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Figure S3. Job’s plots according to the method for continuous variations. The total concentration of HNA and Cu2+ is 10 μM. The intensities were recorded at 513 nm, excitation at 411 nm.
Figure S4. Benesi-Hildebrand plot of HNA (10 μM) based on 1:1 binding stoichiometry with Cu2+ ions. The intensities were recorded at 513 nm, excitation at 411 nm.
Figure S5. Linear relationship between fluorescence intensity of HNA (1 μM) at 513 nm versus the concentration of Cu2+ (0-3.5 μM) in DMF-HEPES buffer (20 mM, pH = 7.4, 3:7 v/v). Excitation was performed at 411 nm.
Sensors 2016, 16, 79; doi:10.3390/s16010079
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Figure S6. Absorption spectra of HNA (10 μM) in DMF-HEPES buffer (20 mM, pH = 7.4, 3:7 v/v) upon addition of various metal ions (30 μM).
Figure S7. The colour changes of HNA (10 μM) in DMF-HEPES buffer (20 mM, pH = 7.4, 3:7 v/v) upon addition of various metal ions (30 μM).
Figure S8. The linear fluorescence responses of HNA-Cu2+ (3 μM) versus low concentration Cys (0–3.3 μM) at 513 nm. Excitation was performed at 411 nm.
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Figure S9. The linear fluorescence responses of HNA-Cu2+ (3 μM) versus low concentration GSH (0– 4.6 μM) at 513 nm. Excitation was performed at 411 nm.
Figure S10. UV-Vis absorption spectra of HNA-Cu2+ (10 μM) in the presence of increasing amount of GSH (40 μM) in DMF-HEPES buffer (20 mM, pH = 7.4, 3:7 v/v).
Figure S11. UV-Vis absorption spectra of HNA-Cu2+ (10 μM) in the presence of increasing amount of Cys (40 μM) in DMF-HEPES buffer (20 mM, pH = 7.4, 3:7 v/v).
Sensors 2016, 16, 79; doi:10.3390/s16010079
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Figure S12. Fluorescence intensity of HNA-Cu2+ (10 μM) at 513 nm in DMF-HEPES buffer (20 mM, pH = 7.4, 3:7 v/v) upon the alternate addition of Cys/Cu2+ with several concentrations ratio (0:0, 20:0, 20:40, 80:40, 80:160, 160:160, 160:320, 320:320, 320:640, 640:640 μM, respectively). Excitation at 411 nm.
Figure S13. Fluorescence intensity of HNA-Cu2+ (10 μM) at 513 nm in DMF-HEPES buffer (20 mM, pH = 7.4, 3:7 v/v) upon the alternate addition of GSH/Cu2+ with several concentrations ratio (0:0, 20:0, 20:40, 80:40, 80:160, 160:160, 160:320, 320:320, 320:640, 640:640 μM, respectively). Excitation at 411 nm.
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3 39 .1
*MSD1 SPC, time=0.386:0.502 of C:\CHEM32\1\DATA\FXM\20141201010.D ES-API, Neg, Scan, Frag: 80
100
Max: 48847
80
60
34 0.1
40
1 71 .1
36 1.1
20
0 100
200
300
400
500
m/z
Figure S14. ESI-mass spectra of HNA. YM-9-M401 #32-41 RT: 0.11-0.13 AV: 10 SB: 9 0.74-0.77 NL: 1.32E5 T: ITMS + c ESI Full ms [180.00-1100.00] 401.08
100 95 90 85 80 75 70 65
Relative Abundance
60 55 403.15
50 45 40 35
402.16
30 25 20 404.13
15 10 5 0
393.33 388.17 389.25 390.15 392.19 394.57
387.22
388
390
392
394
396.16 396
406.14
400.46 398.15 399.25 398
400
408.01
402
404
406 m/z
408
409.19 410.46 410
411.93 412
414.33 415.22 414
417.11 418.31 419.29
416
Figure S15. ESI-mass spectra of HNA-Cu2+ ensemble.
418
420
422.33 422
424.15 424
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20151221-MQT-M401-NEG #72-79 RT: 0.22-0.24 AV: 8 SB: 4 0.04-0.05 NL: 2.96E3 T: ITMS - c ESI Full ms [120.00-1000.00] 100
339.05
95 90 85 80 75 70 65
Relative Abundance
60 55 50 45 40 35 30 25
340.09
136.79
401.05
20 180.84 15 10 5
169.94 138.78
439.02 466.03 188.84
234.76
265.72 282.04 313.54 328.85
0 150
463.94
403.06
182.84
156.87
200
250
300
361.08 377.19 350
424.13 400
460.95 450
600.97 500.98 518.90
467.00 500
549.03
588.24
550
m/z
Figure S16. ESI-mass spectra of HNA-Cu2+ ensemble in the presence of Hcy.
603.96
600
