a Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest Universityï¼,. Ministry of Education, College of Pharmaceutical Sciences, ...
Electronic Supplementary Material (ESI) for Analytical Methods. This journal is © The Royal Society of Chemistry 2014
SUPPORTING INFORMATION for A new spectrofluorometric method for pyrophosphate assay based on the fluorescence enhancement of trypsin-stabilized copper clusters
Wei Wang,a Lei Zhan,b Yu Qing Du,a Fei Leng,b and Cheng Zhi Huang*a,b a
Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University）, Ministry of Education, College of Pharmaceutical Sciences, Southwest University, Chongqing 400716, P. R. China. b College of Chemistry and Chemical Engineering, Southwest University, Chongqing 400715, P. R. China.
Fluorescence intensity
4400 4000 3600 3200 2800 2400
CuNCs CuNCs+PPi
2000 1600 3.5
4.0
4.5
5.0
5.5
6.0
pH Fig. S1 Effect of acidity on the fluorescence intensity of this system in CH3COOH-CH3COONa buffers (pH: 3.6, 4.0, 4.4, 4.8, 5.2, 5.6, 5.8).
2000
F- F0
1600 1200 800 400 0
30
4
40
o
50
60
Temperature ( C)
Fig. S2 Effect of temperature (4, 30, 40, 50, 60 oC) on the fluorescence intensity of this system in CH3COOH-CH3COONa buffer (pH: 5.2).
1800 1500
F- F0
1200 900 600 300 0
0
10
20
30
60
120
180
Time (min) Fig. S3 Effect of reaction times (0, 10, 20, 30, 60, 120, 180 min) on the fluorescence intensity of this system in CH3COOH-CH3COONa buffer (pH: 5.2).
Fig. S4 TEM images (scale bar: 20 nm) of the CuNCs before(A) and after(B) reacting with PPi (10000 M) in CH3COOH-CH3COONa buffer (pH: 5.2). Table S1 Photophysical data for the CuNCs before and after reacting with different concentrations of PPi. Fluorescence
Quantum yield
Radiative
lifetime（ns）
（%）
decay rate constant
CuNCs
4.1
1.0
0.2439
CuNCs+2500 M PPi
3.9
1.1
0.2821
CuNCs+20000 M PPi
3.6
1.3
0.3611
Sample
Table S2 The average hydrodynamic diameters of CuNCs before and after reacting with different concentrations of PPi in CH3COOH-CH3COONa buffer (pH 5.2). Samples
hydrodynamic diameter (nm)
CuNCs
1835
CuNCs+625 M PPi
1166
CuNCs+10000 M PPi
637.6
CuNCs+20000 M PPi
484.8
