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European Journal of Immunology Supporting Information for DOI 10.1002/eji.201545920 Silvia Preite, Dirk Baumjohann, Mathilde Foglierini, Camilla Basso, Francesca Ronchi, Blanca M. Fernandez Rodriguez, Davide Corti, Antonio Lanzavecchia and Federica Sallusto Somatic mutations and affinity maturation are impaired by excessive numbers of T follicular helper cells and restored by Treg cells or memory T cells

C 2015 The Authors. European Journal of Immunology published by WILEY-VCH Verlag

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Supporting Information

Somatic mutations and affinity maturation are impaired by excessive numbers of T follicular helper cells and restored by Treg cells or memory T cells

Silvia Preite, Dirk Baumjohann, Mathilde Foglierini, Camilla Basso, Francesca Ronchi, Blanca Maria Fernandez Rodriguez, Davide Corti, Antonio Lanzavecchia, and Federica Sallusto

1

A WT spleen

IgM MAdCAM-1 CD3e

B

Bone marrow

Blood

Spleen

Lymph node

8.17 8.51 T1 T2

4.03 7.02

3.02 6.41

0.22 4.27

86.1

87.2

1.54 7.87

2.88 8.68

Spleen 8.18 MZ

10.3 9.73

WT FO

93 7.51

0.17 5.51

Cd3eā€“/ā€“

IgM

85.1

92

CD21

87.8

73.8

CD23

E

WT

101 0

10 1