Cameron Alexander d. ,. Kevin M. Shakesheff d. , and Brian R. Saunders a,. * a. Biomaterials Research Group, The School of Materials, The University of ...
Supporting Information for: Thermally-triggered
assembly
of
cationic
graft
copolymers
containing 2-(2-methoxyethoxy)ethyl methacrylate side chains Nabilah Shahidana, Ruixue Liua,b, Francesco Cellesic, Cameron Alexanderd, Kevin M. Shakesheffd, and Brian R. Saundersa,* a
Biomaterials Research Group, The School of Materials, The University of Manchester, b
Grosvenor Street, M13 9PL, UK.
School of Material and Chemical Engineering, Zhengzhou
University of Light Industry, Zhengzhou, 450002, P.R. China.
c
School of Pharmacy, The
University of Manchester, Oxford Road, M13 9PT, U.K. d School of Pharmacy, The University of
Hydrodynamic diameter / nm
Nottingham, University Park, Nottingham, NG7 2 RD, UK.
200
Precipitation
150 100 0.05M 50
0.001M 0
0 0
10
20
30
40
50
Temperature / oC Fig. 1S. Variation of hydrodynamic diameter with temperature for M1-PMA101 in the presence of different concentrations of NaCl (shown). The dispersion precipitated in the presence of 0.05 M NaCl when the temperature exceeded 20 oC.
Hydrodynamic diameter / nm
180 160 140 120 100 80 60 40 20 0
MI1-PMA101 M1-PMA52
0
20
45
10
20
20
20
45
30 40 50 Time / min
60
45
70
Fig. 2S. Variation of hydrodynamic diameter for M1-PMA52 and M1-PMA101 with temperature cycling. The measurements commenced at 20 oC and increased to 45 oC and then returned to 20 oC.
(b)
(a) 50 nm
50 nm
(c)
200 nm
Fig. 3S. TEM images of stained micellar aggregates of (a) M1-PMA29, (b) M1-PMA101 and (c) a lower magnification image of M1-PMA52. The samples were deposited and stained using phosphotungstic acid. The small white particles in (a) and (b) originate from the phosphotungstic acid which acts as a positive stain for the micellar aggregates. Aggregates are highlighted with arrows.
Table 1S. Measured and calculated sizes for unimolecular micelles at approximately 20 oC.
Code
d20 / nma
(dm(uni) + Lm(uni))b / nm
d20/(dm(uni) + Lm(uni))
M1-PMA18
26
20
1.3
M1-PMA29
54
25
2.1
M1-PMA52
49
36
1.4
M1-PMA101
110
60
1.8
M3-PMA69
77
45
1.7
Hydrodynamic diameter measured at 20 oC, b dm(uni) and Lm(uni) are the calculated diameters and lengths, respectively, for cylinders of the M(x/y)-PMAn copolymers considered. The calculations for these values were based on the compositions (Table 1) and also a repeat unit length of 0.24 nm. a
