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Figure S1. Mixed acid-2,3-butanediol pathway (dashed lines represent the pathways that are active only under the aerobic conditions). TCA: tricarboxylic acids ...

Additional file 1-Metabolic pathways of acetoin and 2,3-butanediol in the bacteria Monosaccharide 1

CO2 12

Phosphoenolpyruvate ADP

CO2

2

ATP 16

H2 GTP

TCA

CoA-SH

CO2

18

Acetyl-CoA

NADH+ H+

3 NAD+ FAD

2CO2

11

NAD+

4

CoA-SH NAD+ CO2 NADH+ H+

14

H2O TPP

Acetylaldehyde

CO2

CoA-SH

9

NAD+

5

Ethanol

7

ATP

Acetate

NADH+ H+ NAD+

Acetyl-phosphate ADP

Fumarate

α-Acetolactate

CoA-SH NADH+ H+

Malate

Pyruvate

8

Pi 6

13

NAD+

Lactate

TPP

17

3

GDP 3 NADH+ H+ FADH2

NADH+ H+ NAD+

PYRUVATE

Formate

Oxaloacetate NADH+ H+

Acetoin

NADH+ H+ NAD+

15

Succinate

10

2,3-Butanediol

Figure S1. Mixed acid-2,3-butanediol pathway (dashed lines represent the pathways that are active only under the aerobic conditions). TCA: tricarboxylic acids cycle, Enzymes: 1, enzymes of glycolysis (and pentose phosphate pathway); 2, pyruvate kinase; 3, pyruvate-formate lyase; 4, acetaldehyde dehydrogenase; 5, ethanol dehydrogenase; 6, phospho-transacetylase; 7, acetate kinase; 8, acetolactate synthase; 9, acetolactate decarboxylase; 10, acetoin reductase (2,3-butanediol dehydrogenase); 11, lactate dehydrogenase; 12, phosphoenolpyruvate decarboxylase; 13, malate dehydrogenase; 14, fumarase; 15, succinate dehydrogenase; 16, formate-hydrogen lyase; 17, pyruvate dehydrogenase multienzyme complex; and 18, citroyl synthetase.

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