Supporting Information Table 1 The serine protease inhibitors ... - PLOS

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TIL. Yes. BmSPI28 BGIBMGA004726. BmSPI29 BGIBMGA007721. BmSPI30 BGIBMGA004736. BmSPI31 BGIBMGA013903. BmSPI32 BGIBMGA013848.
Supporting Information Table 1 The serine protease inhibitors in the silkworm Name

Name in silkDB

SPI domain

BmSPI1 BmSPI2 BmSPI3 BmSPI4 BmSPI5 BmSPI6 BmSPI7 BmSPI8 BmSPI9 BmSPI10 BmSPI11 BmSPI12 BmSPI13 BmSPI14 BmSPI15 BmSPI16 BmSPI17 BmSPI18 BmSPI19 BmSPI20 BmSPI21 BmSPI22 BmSPI23 BmSPI24 BmSPI25 BmSPI26 BmSPI27

BGIBMGA009953 BGIBMGA007720 BGIBMGA010212 BGIBMGA013852 BGIBMGA013849 BGIBMGA007729 BGIBMGA013903 BGIBMGA013904 BGIBMGA001983 BGIBMGA010214 BGIBMGA010213 BGIBMGA010216 BGIBMGA002368 BGIBMGA013958 BGIBMGA008831 BGIBMGA003292 BGIBMGA008830

Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin Serpin

BGIBMGA004735 BGIBMGA008827

BGIBMGA008828 BGIBMGA008826 BGIBMGA011587

Numbers of SPI domain 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1

Chr.#

Scaffold #

Starting point

/ 15 7 28 28 15 28 28 19 7 7 7 / 28 3 / 3 / 22 3 15 / 22 3 3 / 23

nscaf2979 nscaf2888 nscaf2986 nscaf3098 nscaf3098 nscaf2888 nscaf3099 nscaf3099 nscaf2204 nscaf2986 nscaf2986 nscaf2986 nscaf2439 nscaf3099 nscaf2925 nscaf2641 nscaf2925 nscaf2641 nscaf2814 nscaf2925 nscaf2888 scaffold2278 nscaf2814 nscaf2925 nscaf2925 scaffold706 nscaf3027

113003 1695601 2025913 198532 156075 1281387 534661 2672749 4280050 2083047 2059013 2098064 2860 567124 475942 21922 461290 1197 364955 436242 3730190 254 377650 445169 383697 32832 4608138

End point 118777 1703011 2049705 199764 157103 1287794 535860 2675709 4285863 2086003 2064447 2104805 4292 568323 483150 28230 468785 7586 383519 442612 3742067 2426 383519 447682 388498 37928 4613908

Chain

EST

Annotation

+ + + + + + + + + + + + + + + + + + +

Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes Yes No Yes

Serpin1 Serpin2 Serpin3 Serpin4 Serpin5 Serpin6 Serpin7 Serpin8 Serpin9 Serpin10 Serpin11 Serpin12 Serpin13 Serpin14 Serpin15 Serpin16 Serpin17 Serpin18 Serpin19 Serpin20 Serpin21 Serpin22 Serpin23 Serpin24 Serpin25 Serpin26 Serpin27

BmSPI28 BmSPI29 BmSPI30 BmSPI31 BmSPI32 BmSPI33 BmSPI34 BmSPI35 BmSPI36 BmSPI37 BmSPI38 BmSPI39 BmSPI40 BmSPI41 BmSPI42 BmSPI43 BmSPI44 BmSPI45 BmSPI46 BmSPI47 BmSPI48 BmSPI49 BmSPI50 BmSPI51 BmSPI52 BmSPI53 BmSPI54 BmSPI55

BGIBMGA004726 BGIBMGA007721 BGIBMGA004736 BGIBMGA013903 BGIBMGA013848 BGIBMGA001066 BGIBMGA003349 BGIBMGA009072 BGIBMGA009095 BGIBMGA009073 BGIBMGA009094 BGIBMGA009092 BGIBMGA009091 BGIBMGA009093 BGIBMGA009075 BGIBMGA009096 BGIBMGA006251 BGIBMGA006235 BGIBMGA004727 BGIBMGA004728 BGIBMGA010892 BGIBMGA010889

BGIBMGA003074 BGIBMGA006340 BGIBMGA001246 BGIBMGA001091

BmSPI56 BGIBMGA005119 BmSPI57 BGIBMGA005130

Serpin Serpin Serpin Serpin Serpin Serpin Serpin TIL TIL TIL TIL TIL TIL TIL TIL TIL TIL TIL TIL TIL TIL TIL Kunitz_BPTI Kunitz_BPTI Kunitz_BPTI Kunitz_BPTI Kunitz_BPTI Kunitz_BPTI Kunitz_BPTI/ Antistasin/WAP Kunitz_BPTI/ WAP

1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 3 1 3 4 6 4 5 1 1 1 1 1 1

22 15 22 28 28 13 15 3 3 3 3 3 3 3 3 3 6 6 22 22 22 22 2 / 4 6 13 13

nscaf2814 nscaf2888 nscaf2814 nscaf3099 nscaf3098 nscaf1898 nscaf2655 nscaf2931 nscaf2931 nscaf2931 nscaf2931 nscaf2931 nscaf2931 nscaf2931 nscaf2931 nscaf2931 nscaf2851 nscaf2851 nscaf2814 nscaf2814 nscaf3005 nscaf3005 nscaf2964 nscaf2818 nscaf2589 nscaf2852 nscaf1898 nscaf1898

504860 1649315 387723 2677905 134013 703219 1012273 936942 942071 919347 912977 894015 881660 899792 551264 958549 1060348 1089671 486479 421025 1142843 1095501 2742907 112890 735178 2595227 11070169 278132

511103 1655134 398842 2680242 135200 705634 1016336 937907 944291 920110 913700 896376 883488 899959 552975 972519 1063762 1100598 490449 435042 1143684 1100551 2744750 115513 735988 2596475 11076436 291974

+ + + + + + + + + + + + + + + +

Yes Yes No Yes Yes Yes Yes Yes Yes No Yes No Yes No Yes Yes No Yes No Yes Yes Yes Yes Yes No No Yes Yes

1/3/2

25

nscaf2823

1469785

1490431

+

Yes

4 /1

25

nscaf2823

1859028

1878038

+

Yes

Serpin28 Serpin29 Serpin30 Serpin31 Serpin32 Serpin33 Serpin34

AAO17293 AAL83944

BmSPI58 BGIBMGA005129

Kunitz_BPTI

8

25

nscaf2823

1825365

1858352

+

Yes

BmSPI59

Kazal

1

20

scaffold826

5493

7786

-

Yes

NP_001037047

BmSPI60 BGIBMGA011573 BmSPI61 BGIBMGA014012 BmSPI62 BGIBMGA003667 BmSPI63 BGIBMGA002723 BmSPI64 BGIBMGA013485 BmSPI65 BGIBMGA009047

Kazal

1

23

nscaf3027

3578987

3579235

+

Yes

NP_001040250

Kazal Kazal Kazal Kazal Kazal

1 1 1 1 3

28 5 5 5 3

nscaf3099 nscaf2674 nscaf2529 nscaf3075 nscaf2931

4075266 3633185 5168618 827184 1782179

4077084 3648429 5181244 849062 1783723

+ + -

No Yes Yes No Yes

BmSPI66 BGIBMGA011574 BmSPI67 BGIBMGA003498 BmSPI68 BGIBMGA013655 BmSPI69 BGIBMGA008205 BmSPI70 BGIBMGA007558 BmSPI71 BGIBMGA004225 BmSPI72 BGIBMGA004226

Kazal

1

23

nscaf3027

3614163

3615180

+

Yes

Kazal Kazal amfpi Kunitz (ITI) Pacifastin Pacifastin

7 9 1 / 2 4

5 / / 15 20 20

nscaf2674 nscaf3087 nscaf2893 nscaf2887 nscaf2780 nscaf2780

+ + 0 +

No Yes Yes Yes No No

Pacifastin

13/11

20

+

Yes

Bowman-Birk

11

10

nscaf2859

732807

824065

+

Yes

BmSPI75 BGIBMGA012631 α-macroglobulin

1

/

nscaf3053

468531

493450

-

No

BmSPI76 BGIBMGA004193 α-macroglobulin

1

19

nscaf2770

1074754

1098181

+

No

BmSPI77 BGIBMGA004536 α-macroglobulin

1

27

nscaf2797

1139344

1159911

+

No

BmSPI78 BGIBMGA006306 α-macroglobulin

1

6

nscaf2852

943084

952132

+

Yes

BmSPI79 BGIBMGA006297 BmSPI80 BGIBMGA008016

1 2

6 9

nscaf2852 nscaf2889

357420 1221751

376290 1224798

+ -

Yes Yes

BmSPI73 BmSPI74 BGIBMGA006856

WAP WAP

5095535 5109274 33689 46217 64178 65058 351002 376921 11962 16806 34504 35673 (nscaf2779) (nscaf2780) nscaf2779-2800 204740 8369

NP_001040294

AY655143

Breugelmans et al., 2009