Materials and methods. Chronic hepatic steatosis and hepatic insulin resistance were induced by high-fat feeding of male C57BL/6Jico mice for 6 weeks.
Diabetologia (2006) 49:3049–3057 DOI 10.1007/s00125-006-0439-5
ARTICLE
Sustained activation of the mammalian target of rapamycin nutrient sensing pathway is associated with hepatic insulin resistance, but not with steatosis, in mice E. Korsheninnikova & G. C. M. van der Zon & P. J. Voshol & G. M. Janssen & L. M. Havekes & A. Grefhorst & F. Kuipers & D.-J. Reijngoud & J. A. Romijn & D. M. Ouwens & J. A. Maassen Received: 10 May 2006 / Accepted: 24 July 2006 / Published online: 28 September 2006 # Springer-Verlag 2006
L. M. Havekes Department of Cardiology and Metabolic Diseases, Leiden University Medical Centre, Leiden, the Netherlands
of male C57BL/6Jico mice for 6 weeks. In addition, acute hepatic steatosis in the absence of insulin resistance was induced by pharmacological blockade of β-oxidation using tetradecylglycidic acid (TDGA). mTOR signalling was examined in liver homogenates. Results High-fat feeding caused obesity (p
