REVIEW: Intracellular. Clinical Chemistry. D. N. Baron ... Fred Cantor, Sidney. Green. Leo L. Stolbach, .... Fred H. Katz and Judith A. Smith. 534. Characterization.
Volume 18
Numbers
1 to 12
January
(( \,
0
llllll
Journal of the American
1972 to December
ee
January 1
INFORMATION FOR AUTHORS
5
REVIEW: Diagnostic Value of Urinary Enzyme Determinations Wolfgang
26
P. Raab
GUEST EDITORIAL: General Problems for Clinical Laboratory Automation Robert
34
Determination
37
Manual and Automated Determination
S. Melville
Sephadex
48
Determination
D. Kinney
of Plasma a-Amino Nitrogen by Atomic Absorption Spectrophotometry Frank F. Hall, Barbara Schneider, Thomas W. CuIp, and Charles of Immunoglobulins J. Savory,
43
and Thomas
Adsorption
of Bilirubin
of SMA-Mark
from
in Unconcentrated
M. G. Heintges,
Cerebrospinal
L. M. Killingsworth,
Neonatal and Adult Serum J. Kapitulnik, S. H. Blondheim,
R. Ratliff
Fluid
and J. M. Potter
and N. A. Kaufmann
X Set Points for Bilirubin by Standard Addition Technique Ronald H. Laessig, Thomas H. Schwartz, and Thomas
A. Paskey
52
Albumin Quantitation by Dye Binding and Salt Fractionation Techniques
57
Estimation of Normal Ranges from a Controlled Sample Survey. I. Sex- and Age-Related Influence on the SMA 12/60 Screening Group of Tests A. H. Reed, D. C. Cannon, J. W. Winkelman, Y. P. Bhasin, R. J. Henry, and V. J. Pileggi
67
Measurement
Don S. Miyada, Vernon Baysinger. Solomon Notrica, and R. M. Nakamura
SCIENTIFIC 73
and Distribution of Zinc, Cadmium, and Mercury in Human Kidney Tissue Hugh D. Livingston
NOTES
Clinical Nephelometry: of Plasma Fibrinogen
1. New, Accurate, Heat-Precipitation
Nephelometric
Method for Rapid Determination
Eugene W. Rice and D. E. R. Muesse 75
Screening of 563 Students for Cholinesterase Variants Charles E. Becker
77 81
Plasma Calcium Assay, with Use of the Jellyfish Protein, Aequorin, as a Reagent K. T. Izutsu and S. P. Felton THE CLINICAL CHEMIST
91
BOOK REVIEWS
J. S. King, Jr. Clara Heise,
Joseph
F. Contrera,
Robert W. Prichard,
and John A.
LETTERS
93
Preparation of Sodium Thymolphthalein Monophosphate, an Economical Substrate for Measurement of Alkaline and Acid Phosphatase Activities
94
“Phosphorylatable
Gary J. Proksch
Buffers” for Serum Alkaline Phosphatase Elias Amador
February 95
GUEST EDITORIAL:
Coming Events CastTheir Shadows Roger J. Williams
97
Study of Optimum Buffer Conditions for Measuring Alkaline Phosphatase Activity in Human Serum Robert
1558
CLINICAL
CHEMISTRY, Vol. 18, No. 12, 1972
B. McComb
and George
N. Bowers,
Jr.
Loft.
1972
February (continued) 105
Identification of Serum Cholinesterase Phosphate Buffers
Fluoride Variants by Differential Phillip
Inhibition in Tris and
J. Garry, George M. Owen, and A. Harold Lubin
110
A Study of Various Electrophoretic Phosphatase lsoenzymes
and Inhibition Techniques for Separating Serum Alkaline
116
Diagnostic Effectiveness of Electrophoresis and Specific Protein Assays, Evaluated by Discriminate Mario Werner, Samuel H. Brooks, and Georg Cohnen
124
Evaluation for Four Reagents Kits and Two Flame Photometers Used to Determine Sodium and Potassium in Serum Edward K. Kim, Leslie D. Waddell, and James E. Logan
129
Gas-Chromatographic
131
Determination of Serum Acid Phosphatase Activity: A Performance Evaluation for Clinical Laboratories of Upstate New York William H. Copeland and Raymond E. Vanderlinde
137
Effect of Some Drugs on Clinical Laboratory Values as Determined by the Technicon SMA 12/60 H. P. Singh, M. A. Hebert, and M. H. Gault
145
Rapid Large-Scale Screening of Blood Proteins by Use of Cellulose Acetate Electrophoresis on a Solid Support Samuel T. Nerenberg, Linda DeMarco, and Carolyn Ganger
150
Instrumental
155
Determination of Ionized Calcium in Serum by Use of an Ion-Selective Electrode. I. Determination Physiologic Conditions, with Comments on the Effect of Food Ingestion and Hyperventilation Bette Seamonds, Javad Towfighi, and Dan A. Aryan SCIENTIFIC
NOTES
161
Tyrosinemia
Induced by a Pyridoxine Antagonist, Desoxypyridoxine E. Joanne Easton, Ian Simpson, J. Kenneth Martin, and Donald J. Campbell
164
Polyethylene Coils for Continuous-Flow Analyzers Elias Amador
167
THE CLINICAL CHEMIST
Roy B. Johnson, Jr.,Karen Ellingboe, and Phyllis Gibbs
Determination
Analysis
of Total Estriol in Amniotic Fluid Joseph C. Touchstone, Taras Murawec, and Ronald J. Bolognese
and Procedural Sources of Error in Determination Robert W. Burnett
of Bile Pigments in Amniotic Fluid of Normal Values under
J. S. King, Jr. LETTERS 171 171 172 172 174
Tableting of Enzyme Control David Bayse and Dorothy Sue Lewis A Simple, Inexpensive Steel Mortar and Pestle for Crushing Large Bones Hans U. Aeschbacher and R. Glenn Brown SRM’s and Verification of Commercial Standards J. Paul Cali Normal Ranges for Serum Creatine Phosphokinase Don S. Miyada, Robert M. Nakamura, and Linda Boyko BOOK REVIEWS Samuel Meites, Theodore Peters, Jr., George Bryson and Fritz Bisohoff, and Morton K. Schwartz
March 179
REVIEW:
Prenatal Diagnosis of Genetic Disorders: Trials and Tribulations Ronald G. Davidson and Mario C. Rattazzi
188
Blood Carboxyhemoglobin: Results with Conventional Standards Compared with Those with a Submlcroliter Reference of Gaseous CO Gunnar Sundstrm
193
Automated
199
Chemical Basis of the Sulfo-phospho-vanlllln Reaction for Estimating Total Serum Lipids Joseph A. Knight, Shauna Anderson. and James M. Rawle
203
SensitIve, Rapid Quantitation
206
Tris/Tris.HCI:
209
Interference
212
Measurement
217
Quantitatlon of Beta Lipoprotein (LDL) Cholesterol by Densitometric Evaluation of Disc Electropherograms Robert F. Moran, William P. Castelli, and Marianne V. Moran
Fluorometrlc
Procedure for Measurement of Creatine Phosphokinase J. A. S. Rokos, S. B. Rosalki, and D. Tarlow
Activity
of Serum and Urinary Protein by Electroimmunodiffusion M. C. B. de Pizzolato, G. B. Del Campo, M. A. Pizzolato, and C. Vergan
A Standard Buffer for Use in the Physiologic pH Range Richard A. Durst and Bert R. Staples in Pisano’s Urinary Metanephrine Assay after Use of X-Ray Contrast Media LaVelI R. Johnson, Max Reese, and Don H. Nelson of Unconjugated
17 s-Estradlol In Plasma by Competitive B. S. Knox and J. T. France
Protein Binding
CLINICAL
CHEMISTRY, Vol. 18, No. 12, 1972
1559
March (continued) 222
Proficiency Evaluation of Clinical Chemistry Laboratories G. F. Grannis,
H..D.
Grumer,
J. A. Loft, J. A. Edison,
and W. C. McCabe
237
SIngle Radial Immunodiffusion
of Undiluted Serum Proteins
239
Automated Direct Method for Measurement of Serum Cholesterol, with Use of Primary Standards and a Stable Reagent
M. C. B. Pizzolato, William
M. A. Pizzolato,
and A. Agostoni
R. Holub and Frank A. GaIIi
244
Effectsof Intra-and Inter-Individual Variationon Distributionsof Single Measurements
250
Computerized Preparation of Two-Way Analysis of Variance Control Charts for Clinical Chemistry J. H. Riddick, Jr., Roger Flora, and Quentin L. Van Meter
258
SeparatIon and Identification of Neutral and Acidic Metabolites In Cerebrospinal Fluid
263
Direct Method for Determining Inorganic Phosphate In Serum with the “CentrifiChem”
266
Test for Mucopolysaccharldoses: Simple Method for Quantitative Estimation of Urinary Glycosaminoglycans
270
SpecIficity of Accepted Procedures for Urine Creatlnine
275
“Glycerate Dehydrogenase” Activity in Acute Myocardial Infarction and Myocardial Ischemla
Eugene
K. Harris and David L. DeMets
L. D. Waterbury
and L. A. Pearce
John A. Daly and Gerhard Ertingshausen Alberto
Calatroni
Sheshadri
Narayanan
M. J. McQueen,
and Harold
D. Appleton
I.W. C. Garland,and H. G. Morgan
280
ImplIcation of Creatinine and Gut Flora in the Uremlc Syndrome: Induction of “Creatlnlnase” in Colon Contents of the Rat by DIetary Creatinine
285 290
An Automatic, Solid-State Direct Readout for the Buchler-Cotlove Chloridometer AlfredZettner and Walter C. Fontana Rapid ExtractIon of Triglycerides from Human Adipose Tissue with Petroleum Ether
293
CASE REPORT: Multiple Myeloma Presenting as Adult Fanconi Syndrome
James
D. Jones and Philip C. Burnett
H. A. I. Newman, Elizabeth Robert
A. Gordon,
Darrol W. Heggen,
N. Headley, J.Stanton King,Jr.,M.
and Martin
D. Keller
Robert Cooper, and John H. Felts
SCIENTIFIC NOTES of Total Urinary Protein by Combined Gel Filtration and Automated Biuret Reaction KarlDoetsch
296
DetermInation
299
DetermInation of Serum or Plasma Glucose on the “AutoAnalyzer Il” by Use of the Hexokinase Reaction
301
A “Wash-Out
305
THE CLINICAL CHEMIST
311
BOOK REVIEWS
G. M. Widdowson Pipet
System” for Sampling Red
Blood
and J. R. Penton
Cells
Richard G. Stoler J. S. KIng, Jr.
Allen H. Reed, C. F.
Strittmatter, Irving
Sunshine,
J. S. King,
Jr., and H.-D. Gruemer
LETTERS
315
Lecithin/Sphlngomyelin Ratio in Respiratory Distress Syndrome R. Gerald Simon
(Reply) Louis Gluck, Richard W. St.Clair
316
Blood Lead Determinations by Atomic Absorption Spectrophotometry, with Use of the Delves Sampling-Cup Technique: Effect of Various Anticoagulants
316
Interference
317
Artlfactual Contamination of Control Serum Extracts in Gas Chromatographic Analyses for Chlorpromazine
StanleyG. Elfbaum, Robert Juliano,Richard E. Macfarland, with Serum
Phosphorus
Determination
by Hycel
and David L. Pfeil
Procedure
Fram R. Dalaland Seymour Winsten Morris A. Spirtes
April 319
EDITORIAL: Abbreviations of Names of Enzymes J. S. King, Jr.
320
REVIEW:
330
A Study of the Problems of Inactivation of Creatine Kinase in Serum Fram R. Dalal, Jonathan Cilley, Jr., and Seymour Winsten
335
Manual Nephelometric Methods for Immunochemical and 1gM in Human Serum
Intracellular
Clinical
Chemistry D. N. Baron
L. M. Killingsworth 1560
CLINICAL
CHEMISTRY,
Vol. 18, No. 12, 1972
Determination and John
of Immunoglobulins
Savory
lgG, IgA,
April (continued) 340
Comparison of Results from the Du Pont “ACA” and Technicon “SMA 12/60” James 0. Westgard and Brenda L. Lahmeyer
349
Direct Colorimetric
Determination
of Hippuric Acid in Urine Katsumaro
352
Rapid, Automated,
Simultaneous
Determination Margaret
355
Semiautomated
Tomokuni
Method for Measurement
Serum Gamma-Glutamyl
Transpeptidase
Simple Fluorometric
and
Mary
of Guanidinosuccinic J. M. Pteau,
I”
H. Cheng
Acid in Serum and N. K. Man
Activity as an indicator of Disease of Liver, Pancreas, or Bone
Gifford
363
Ogata
of Serum CO and Chloride with the “AutoAnalyzer A. Kenny
P. P. Kamoun,
358
and Masana
Lum and S. Raymond
Gambino
Method for Estimating Practolol [1-(4-Aminophenoxy)-3-isopropylaminopropan-2-olJ
In Blood and Urine Gunter
366
Alkaline Phosphatase:
Bodem
Charles
G. Massion
374
An L-Aspartate : 2-Oxoglutarate Aminotransferase Preparation and Characterization
384
Use of Thin-Layer Chromatography Routine Clinical Laboratory
Robert
A. Chidsey
and Jean
Kingsbury
Frankenfeld
Reference Material from Human Erythrocytes:
Rej, Raymond
E. Vanderlinde,
and Charles
on Silica Gel for Serum Lipid Fractionation Antonio
SCIENTIFIC
and Charles
Lability in Fresh and Frozen Human Serum and in Lyophilized Control Material
Chedid,
Peter
Haux,
and Samuel
F. Fasce,
Jr.
and Measurement
in the
Natelson
NOTES
391
Quality Control of an Automated Differential L-Phenylalanine Inhibition
393
Elimination of Dextran Interference
396
THE CLINICAL CHEMIST
Isoenzyme Assay of Alkaline Phosphatase, with Use of
Fred Cantor,
Sidney
Green.
Leo L. Stolbach,
and William
H. Fishman
in Serum Protein Determinations John J. Moore and Sylvan N. Sax J. S. King, Jr.
401
BOOK REVIEWS J. S. King. Jr., Elliot S. Vesell, and R. L. Dryer LETTERS
403
Intravenous
Vitamins and Clinical Laboratory Tests Richard T. O’KeIl, Lois Mantzey, Donald F. Knepper, and Joseph R. Elliott
404
CPK Determination:
A Correction S. B. Rosalki, and D. Tarlow
May
462
of Psychopharmacologic Agents in Cases of Drug Abuse David Sohn and JuliusSimon Carbon Rod Atomizer Applied to Measurement of Lead in Whole Blood by Atomic Absorption Spectrophotometry N. P. Kubasik, M. T. Volosin, and M. H. Murray Semiquantitation of Leucine, Isoleucine, and Valine by Thin-Layer Chromatography in Management of Maple-Syrup Urine Disease Richard J. AlIen, H. Jeane Frey, LaDona M. Fleming, and Clyde L. Owings Cellulose Acetate Electrophoresis of Alkaline Phosphatase Isoenzymes in Human Serum and Tissue H. A. Fritsche, Jr. and H. R. Adams-Park Clinical Evaluation of a Modified “Oxford T4-by-Column” Method for Serum Thyroxine Marcia Lee, Norbert W. Tietz, and Charles J. Martinez Successive Determination of Na, C1, and H Activities on the Skin Surface with Ion-Selective Electrodes Marvin Green, Hans Behrendt, and Gloria Libien Automated Sample-Reagent Loader for Use with the GeMSAEC Fast Analyzer C. A. Burtis, W. F. Johnson, J.C. Mailen,and J.E.Attrill Microfluorometry of Glucose-6-Phosphate Dehydrogenase and 6-Phosphogluconate Dehydrogenase in Red Cells Mei Lee Lowe, Angelo F. Stella, Beatrice S. Mosher, Jerry B. Gin, and James A. Demetriou Laboratory for Preparation of High-Purity Clinical Chemicals Morris Zief and Alexander C. Nesher Spectrophotometry of Proline in Plasma and Urine Jesse F. Goodwin Spectrophotometric Micromethod for Measuring Cholinesterase Activity in Serum or Plasma Tetsuo Uete,Yoko Miyamato, Mieko Ohnishi,and NorikoShimano CASE REPORT: Symptomatic Porphyria in a Case of Felty’s Syndrome. I. Clinical and Routine Biochemical Studies L. Eales, W. G. Sears, K. B. King, M. J. Levey, and C. Rimington II. Biochemical Investigations
471
SCIENTIFIC NOTES Hydrochlorothiazide Interference
405
410 413 417 422 427 433 440 446 449 454
459
REVIEW:
Rapid Identification
C. Rimington, W. G. Sears, and L. Eales with Urinary Estriol Determination Arthur F. Rosenthal and Marie R.Tomson CLINICAL
CHEMISTRY,
Vol. 18, No. 12, 1972
1561
May (continued) 473
Activation of Serum Creatine Kinase by Dlthlothreitol
476
Direct Fluorometric Determination
475
CORRECTIONS
480
THECLINICALCHEMIST
487
LETTERS Cholinesterase
487
Robert I. EIlin A Multiple Sampling Device for the AutoAnalyzer
488
o-Toluidine Methods for Glucose:
488
On Normal Values
489
Sickle-Cell Anemia:
William
A. Warren
of Urea in Urine Joon H. Rho
J.S. King, Jr.
Determination
(cont.)
P. Z. Sobocinski and R. P. McDevitt
Effect of Lipemia
Christopher S. Frings and Cecelia Queen
490 492 492 492 494
Joseph S. Annino and Louis A. Williams A Simplified Screening Procedure A. P.Rosenberg, Norma Byrnes,and Leona Sexton Rapid TLC Separation and Detection of Lecithin and Sphlngomyelin in Amniotic Fluid Emily H. Coch and Gerald Kessler “Caveat Emptor”-.-.Uricase Nathan Gochman and Joan M. Schmitz Filter Paper Contamination: Sn and Ca Martin Marcus and Seymour Kleinberg Nucleotide Separation on Alumina GregoryS.Duboff BOOK REVIEWS Allen H. Reed, Richard W. St. Clair, H.-D. Gruemer, Thomas A. Rejent, and James 0. Westgard
Ellen Borenfreund,
George Bryson,
June 499
Estimation of the Concentration Ultracentrifuge
503
Coulometric
509
516 519 523 528 534
of Low-Density Lipoprotein Cholesterol in Plasma, Without Use of the Preparative
William T. Friedewald, Robert I. Levy, and Donald S. Fredrickson of Activity of Acid or Alkaline Phosphatases in Serum Marvin A. Brooks and William C. Purdy Simple Automated Determination of Serum or Plasma Glucose by a Hexokinase/Glucose-6-Phosphate Dehydrogenase Method William E. Neeley Ultraviolet Spectrophotometric Measurement of Chlordiazepoxide in Plasma Peter Jatlow Nucleic Acid Concentrations in Normal Human Plasma
Determination
Richard C. Kamm and Albert C. Smith
Optimal Conditions for the Kinetic Assay of Serum Glutamate Dehydrogenase Activity at 37#{176}C Graham Ellis and David M. Goldberg Radioimmunoassay of Angiotensin I: Comparison of Two Renin Activity Methods and Use for Other Measurements of the Renin System Fred H. Katz and Judith A. Smith Characterization of Electrophoretic Lipoprotein Fractions: lmmunochemical and Electron Microscopic Studies Mario
5
Werner
and Albert
L.
Jones
548
Factors Affecting the Radioimmunoassay of Digoxin Eugene Cerceo and Cipriano A. Elloso Automated Method for Micro-Scale Determination of Serum Carbon Dioxide R. E. Sterling and 0. R. Flores New Procedure for Purifying and Crystallizing Alkaline Phosphatase from Human Intestinal Mucosa
554
Direct-Contact
563
SCIENTIFIC NOTES Automated Determination
Sheshadri Narayanan and Harold D. Appleton
(Membraneless)
Hemoperfusion through Oils M. A. Evenson and D. de Vos
565
of Serum Oxytocinase Activity J. A. B. M. Peeters Detection of Patients with Low Serum Cholinesterase Activity:
567
Low AspartateTransaminase
568
A Linear Absorbance
Inadequacy of “Acholest”
Albert A. Dietz, Herbert Rubinstein, and Tina Lubrano
Method
Activity in Serum of Patients Undergoing Chronic Hemodialysis
Paul L. Wolf, Dorothy Williams, Norman Coplon, and Alan S. Coulson
Converter for Continuous-Flow Colorimeters Leonard Walker and Elias Amador PROGRAM
571
SCIENTIFIC
585
THE CLINICAL CHEMIST J. S. King, Jr.
591
LETTERS Improved Direct Automated
591
Effect of Phenazopyridine
592
Role of State Health Departments
Method for Serum Urea Nitrogen C. A. Robinson, Jr. and H. 0. Jordan Hydrochloride (“Pyridium”) on Determination of Ethchlorvynol (“Placidyl”)
Jerald W. Cantrell, Nancy E. Hopkins, Cecelia A. Queen, and Christopher S. Frings 593
in Testing Proficiency of Drug Abuse Toxicology for Intrastate H. E. Sine and M. H. Murray Methyl Orange as a Screening Reagent for Organic Bases
1562
CLINICAL
Joseph R. Monforte and Irving Sunshine
CHEMISTRY, Vol. 18, No. 12, 1972
Clinical Laboratories
June (continued) 593
Mercury Pollution from the Laboratory:
A Correctable
Alex
Kaplan
Problem
and William
593
Use of p-Nitrophenyl Phosphate Dicyclohexylammonium Determination on the SMA 12/60
594
Gas Chromatographic
E. Philip
Halpern,
L. Gyure
Salt as Substrate for Alkaline Phosphatase S. J. Rosoff, and S.
Weiner
Assay of Estriol
Alan Barbour
(Reply)
Joseph C. Touchstone
594
Waste Disposal in Clinical Laboratories
595 596
Erroneous Results for Total Serum Lactate Dehydrogenase (LD) Activity when Assayed with Substrate Buffered at pH 10.0 Ted W. Fendley, Katharine Jacobs, Ralph T. Dunn, and Christopher S. Frings The Heath Recorder as a Replacement in the AutoAnalyzer System
597
BOOK REVIEWS
Desider A. Pragay
George
M. Katz and Arthur
L. Levy
Lena A. Lewis, J. S. King, Jr., Nathan Gochman, Irving Sunshine, Russel 0. Bowman, Samuel John H. Felts, and Jesse F. Goodwin
Noel R. Rose, Merle A. Evenson, Meites, Theodore Peters, Jr.,
July 601
Simplified Serum Phosphorus Analyses by Continuous-Flow Ultraviolet Spectrophotometry
605
Estimation of Normal Ranges and Cumulative Proportions by Transforming
Elias Amador Eugene
613
Urinary Cystathionine,
Catecholamine,
and Jackie
K. Harris
and David
M. Fleisher,
616
Automated Method for Screening Urine for Amphetamine
621
Accelerated
Procedure
Observed Distributions
to Gaussian Form
L. DeMets
and Metabolites in Patients with Neuroblastoma L. Helson,
Single-Column
Urban
E. R. Rutter for Automated
V. Bethune,
M. L. Murphy,
and M. K. Schwartz
and Some Related Primary Amines
Measurement
of Amino
Acids in Physiological
Fluids
Klara Efron and Paul L. Wolf 625
Some Technical Aspects of Quantitative
Immunoelectrophoresis
Janet
630
Some Methodological
Modifications of the Technicon “SMA 12/60 AutoAnalyzer” Tevtik
643
of Human Serum and Cerebrospinal
Rapid Gas Chromatographic
Fluid
M. Fuller and J. W. Keyser K. Bigat
Measurement
and Abraham
System
Saifer
of Plasma Procainamide
Concentration
J. Atkinson,
Parker,
Arthur
Jr., Michele
647
Determination
654
Assay of Human Plasminogen in Plasma by Affinity Chromatography
658
A Computer Program for Calculating Blood Acid-Base Parameters
and John
Strong
of Serum Albumin with the “SMA 12/60” by a Bromcresol Green Dye-Binding Method James
0. Westgard
and Michael
Raymond P. Zolton, Edwin T.
A. Poquette
Mertz,
and Henry
T. Russell
on an Olivetti “Programma
101” Desk Computer
J#{216}rgen Hardt
662
SCIENTIFIC NOTES Separation of lsoenzymes of Alkaline Phosphatase by Substrate-Gel Douglas
666
P. Rhone
Rapid Estimation of Fibrinogen in Fibrinolytic and Nonfibrinolytic Arthur
Imprint after Electrophoresis
and Florence
A. Rosenberg
States
and Richard
A. Peabody
668
Continuous-Recording
671
Exploratory Experiments in Direct Evaluation of Renal Metabolic Capabilities Sushil S. Lacy and J. Stanton King, Jr. Improved Determination of Total Serum Lipids by the Sulfo-Phospho-Vanillin
Flame Photometry of Sodium and Potassium in Serum Elias Amador,
673
Christopher
675
Fluorometric
Assay of Diphenylhydantoin
677
Detection of Interrupted
Ronald
L. Cechner,
S. Frings,
and James
Ted W. Fendley,
J. Barklow
Reaction Ralph
T. Dunn,
A. Dill and Anthony
A. Queen
J. Glazko
Bubble Flow in the Technicon Automated Immunoprecipitin Robert
MEETING,
and Cecelia
in Plasma or Whole Blood
Wesley
24th NATIONAL
on Cellulose Acetate
M. Mizuno
J. Hansen,
Anthony
R. Tschida,
System
and Harold
Markowitz
AACC
679
Symposia Lecturers and Abstracts
687
Abstracts of Papers
725
THE CLINICAL CHEMIST (including Report of the Papers Committee)
736
BOOK REVIEWS
J. S. King,
Jr.
W. E. Cornatzer, J. I. Routh
Irving
739
LETTERS Improved Screening Tests for Porphyrin
739
At What Temperature
740
Abbreviations for Enzyme Names
740
Comparison of Two Radioassay Methods for Vitamin B12
741
Urinary Urocanic Acid Determination:
Janet
Nutter
Sunshine,
and Robert
Elias Amador,
Charles
L. Woronick,
W. M. Wise,
and
F. Labb#{233}
Should Enzymes of Clinical Interest be Assayed? M. Roth Waldo Norman
E. Cohn P. Kubasik
and Martin
H. Murray
A Criticism
Ralph Chayen, Arieh Harell, and Edna Neufeld
(Reply) D. Barbieri,
741
I. A. da Silva,
and J. Nicolau
Evaluation of a New Non-Extraction Technique for Serum Thyroxine Measurement Lowell
B. Foster
and Deborah
L. MacFadden CLINICAL
CHEMISTRY,
Vol. 18, No. 12, 1972
1563
August Fourth Annual Symposium on Advanced Analytical Concepts for the Clinical Laboratory, March 9 and 10, 1972. Sponsored by the Oak Ridge National Laboratory and the National Institute of General Medical Sciences 743
Introduction
744
I. ADVANCED CONCEPTS Computerized AutomatIon In AcquisitIon and Processing of Patterns from High-Pressure Ion-Exchange Chromatography
Charles D. Scott and Robert S. Melville
B. J. Cerimele,
749 753 762
D.C. Clapp,
G. C. Cokinos,
B. D. Obermeyer,
and R. L. Wolen
DynamIc Introduction of Whole-Blood Samples Into Fast Analyzers Charles D. Scott and James C. Mallen Development of a Miniature Fast Analyzer C.A. Burtis,J.C. Mallen,W. F. Johnson, C. D. Scott, T. 0. Tiffany, and N. G. Anderson Development of a PortableData Processor wIth MechanicalData Output for Use wIth a Miniature Fast Analyzer W. F. Johnson, J. C. Maileri, C. A. Burtis, T. 0. Tiffany, and C. D. Scott II. ANALYTICAL SYSTEMS
767
Simultaneous
Multicolumn
Operation
771
MechanIzed,
775
A
778
Chromatographlc
783
Development of Automation of Differential Leukocyte Counts by Use of Cytochemlstry Alex M. Saunders AutomaticSystemfor Urine Analysis. I. System Design and Development Anton H. (Tom) Clemens and Richard L. Hurtle
Direct Digital-Control
of the UV-Analyzer for Body Fluids W. Wilson Pitt, Jr., Charles D. Scott, and Guy Jones, Jr. Pipette for Automation of Ultramlcro Chemical Analyses
H. Harold Nishi
Systemfor Computer Analysis of Kinetic Enzyme Date from a Modified Beckman DSA-560 J. Richard Pearson, Edward R. Pinfield, and Daniel Cooper Analysis of Naturally Fluorescing Compounds: I. Rapid Analysis of Nanogram
In Physiologic Fluids
Amounts
of lndol.s
D. D. Chilcote and J. E. Mrochek
789 794
II. III.
APPLICATIONS
EvaluatIon of the System
C. Bradley Hager, John R. Brown, and J. M. Botero AND EXPERIMENTAL RESULTS
800
Combined Use of Gas Chromatography, Mass Spectrometry, and Computer in Diagnosis and Studies of Metabolic Disorders E. Jellum, 0. Stokke, and L. Eldjarn
810
Gas-Liquid Chromatographic
850
Analysis for Purine and Pyrimidine Bases In Hydrolysates of NucleIc Acid Duane B. Lakings and Charles W. Gehrke Countercurrent Chromatographic Separation of Catecholamine Metabolltes from Urine Robert E. Hurst and Yoichiro Ito Identification and Biochemical Significance of Substituted Furans in Human Urine J. E. Mrochek and W. T. Rainey, Jr. Enzymatic Kinetic Rate and End-Point Analysis of Substrate, by Use of a GeMSAEC Fast Analyzer T. 0. Tiffany, J. M. Jansen, C. A. Burtis, J. B. Overton, and C. D. Scott KInetic Method for Determining Acid Phosphatase Activity In Serum with Use of the “CentrifiChem” Diane L. Fabiny-Byrd and Gerhard Ertingshausen Nonlinear Regression Analysis Approach for Determining “True” Lactate Dehydrogenase Activity In Serum with the Centrifugal Analyzer (“Rotochem”) Bernard E. Statland and Allan L. Louderback The Commitment of the Clinical Laboratory to Improve Health Care
854
SCIENTIFIC NOTE Precautions in Use of Soft-Glass Disposable Pipets in Clinical Analyses
858
THE CLINICAL CHEMIST
866
BOOK
814 821 829 841 845
D. S. Young Patrick
G. McCormick,
R. W. Burke,
and Basil T. Doumas
J. S. King, Jr. REVIEW
Morton K. Schwartz
LETTERS
867 867 867
Non prostatic Formalin-Resistant
Acid Phosphatase in the Tasmanian Devil R. S. Parsons and E. R. Guiler Practical Aspects of Automating an Ammonia-Specific Electrode Robert L.Coleman The Problem of Chlorine in Distilled Water H. Bruce Collier and Robert A. Stinson
September 869
REViEW: Organic Polymer Biocompatibility
and Toxicology
Fritz Bischoff
895
Negative Interference by Residual Proteins in the Supernatant Fluid Used in Radiolsotopic Assay of Serum Vitamin B12
Esper Mortensen
1564
CLINICAL
CHEMISTRY, Vol. 18, No. 12, 1972
September (continued) 901
Factors Affecting
the Results of Serum Cholesterol Determinations: An Interlaboratory Charles F. Fasce, Jr. and Raymond E. Vanderlinde
Evaluation
909
Comparison of Manometric andInfrared Methodsfor DeterminingCarbonMonoxidein Blood Loren G. Myhre
911
Rapid, Simplified Method for Measuring Total Hepatic Cholesterol Herbert K. Naito and Lena A. Lewis
915
Reporting Terminal for Sequential Multiple Analysis and a Positive Identification System for Serum Specimens James H. Kaufman
918
Evaluation of a New Radiolmmunoassay Method for Carcinoembryonic in Plasma,with Use of Zirconyl Phosphate Gel
923
Determination
Antigen
Tsann Ming Chu and Gustavo Reynoso
of Copper In Fingernails by Atomic Absorption with the Graphite Furnace William
928
Multichannel
Analyzer
Featuring
Ultrahigh
B. Barnett Stability
and HerbertL.
Photometry:
Kahn Application
to Enzyme
Determination
Harry L. Pardue and Michael M. Miller 934
SpotTestfor Detectionof SicklingHemoglobin Sally Kelly and Lucille Desjardins
937
New Automated
Method for Measuring Thiamine (Vitamin B1) in Urine OmerPelletierand Ren#{233} Mad#{233}re
943
Application of a New Peroxide Indicator Reaction to the Specific, Automated Determination of Glucose with Glucose Oxidase Nathan Gochman and Joan M. Schmitz
951
Isolation of 2-Acetamido-1--(L--Aspartamido)-1,2-Dideoxy-D-Glucose Barbara
956
O’Neill
Modified Method for Estimating the Phosphatidyl Choline:Sphingomyelin Ratio in Amniotic Fluid, and Its Use in the Assessment of Fetal Lung Maturity Laszlo Sarkozi,
961
from Normal Human Urine
Rowley and Paul B. Hamilton
Hanna
N. Kovacs,
Howard
A. Fox, and Thomas
Kerenyi
Urea, Urease, Cyanate, and the Sickling of Hemoglobin S
Robert M. Nalbandian, Bruce M. Nichols. Edward J. Stehouwer, and Frank R.Camp,Jr. 965
Automated Vitamin C Analysis
968
Comparison of Ascorbic Acid Concentrations in Whole Blood Obtained by Venipuncture or by Finger Prick Daniel W. Bradley, James E. Maynard, and Gladys Emery
971
Glycosidases in Normal Human Leukocytes and Abnormalities in GM1-Gangliosidosis Jeffrey Hindman and Edward Cotlier
976
Determination of Glucose in Serum and Whole Blood: Statistical Relationships between Values Obtained by Different Methods
980
Photometric Assay of Dopamine-9-HydroxyIase
Hans U. Aeschbacher and R. Glenn Brown
David G. Geeting, Charles A. Suther, and Philip Sylbert Toshiharu
984
Determination
of Phenylbutazone
987
Rapid Spectrofluorometric
996
1001
Measurements
Udenfriend
Assay for Total Bile Salts in Bile J. Mroszczak
and Sidney
Riegelman
of Nickel in Serum and Urine as Indices of Environmental Exposure to Nickel Michael D. McNeely, Maria W. Nechay, and F. William Sunderman,
Jr.
Simplified Colorimetric Determination of Thiocyanate in Biological Fluids, and Its Application to Investigation of the Toxic Amblyopias A. R. Pettigrew and G. S. Fell Modified
Automated
Determination
of 2,3-Diphosphoglycerate Kenneth
1005
and Sidney
in Plasma Eberhard J#{228}hnchen and Gerhard Levy Edward
992
Activity in Human Blood Nagatsu
in Whole
Blood
F. Atkinson
CritIcal Evaluation of a New Flame Emission Analysis System, the ‘Klina Flame” Richard
J. Schlesinger,
Raymond
1009
An Error-Producing
1013
Development and Evaluation of a High-Speed Continuous-Flow
A. Lesonsky,
and
Robert
Lottritz
Interaction in an Automated Method for Measuring Cholinesterase Activity in Blood Robert I. Ellin, William A. Groff, and Andris Kaminskis M. A. Evenson
Analyzer
and M. A. Olson
SCIENTIFIC NOTES 1019
Simplified
Rapid Electroimmunodiffusion Ernest
1021
Multi-Antisera
Technique for Measuring a1-Antitrypsin In Serum R. Manildi
Applicator for Use in Solid-Support Samuel
Immunoelectrophoresis
1. Nereriberg,
Carolyn
Ganger,
on Cellulose Acetate and Linda
CLINICAL
DeMarco
CHEMISTRY,
Vol. 18, No. 12, 1972
1565
September (continued) 1024
Panel Discussion:
International
1023
CORRECTIONS
1031
THE CLINICAL CHEMIST
1034
BOOK REVIEWS
Seminar and Workshop on Enzymology Norbert W. Tietz and Albert Weinstock
J.S.
King,Jr.
Jack M. Becktel, C. M. Heise, Russel 0. Bowman, and P. John Pegg LETTERS 1037
Inaccurate Calcium Standards in Hospital Laboratories Desider A. Pragay, Carson L. Cross, and Max E. Chilcote
1039
Effect of Drugs on Results of Laboratory Tests Richard 1. O’KeU, Donald F. Knepper,
1039
Vitamin Interference
1039
Distribution of Diazepam in pH-Controlled
Lois Mantzey, and Joseph R. Elliott
with BSP-Retention Test Ruth D. McNair
Liquid Extraction
K. Andrew,
R. N. Gupta,
and P. M. Keane
October
Bibliography: Drug Interferences with Clinical Laboratory Tests 1041
Effects of Drugs on Clinical Laboratory Tests D. S. Young, D. W. Thomas, R. B. Friedman, and L. C. Pestaner
1043
Acknowledgments
1044
Generic/Proprietary
1060
Abbreviation and Short-Form
1063
Alphabetic Listing by Drug Name
1166
Alphabetic Listing by Laboratory Test
1269
References
1304
Special
Drug Name Directory
Directory
Announcements
November 1305
GUEST EDITORIAL:
StandardIzatIon
of Enzyme Assays Hans
Ulrich
Bergmeyer
1312
An Improved Delves Cup Atomic Absorption Procedure for Determination Eugene D. Olsen and Peter I. Jatlow
1318
Automated Procedure for Assay of 2,3-Diphosphoglycerate Inorganic Phosphorus
of Lead in Blood and Urine
in Red Cells by Measuring Enzymaticaily
Released
James B. Powell, Clarence E. Emery, and Gaylon A. Peyton
1323
Kinetic Nephelometric
Procedure for Measurement
of Amylase Activity in Serum
1326
Rapid Analysis for Total Mercury in Urine and Plasma by Fiameless Atomic Absorption Analysis Norman P. Kubasik, H. E. Sine, and Michael T. Volosin
1329
Fluorometric Determination Chromatography
1334
Use of the Du Pont “Automatic Clinical Analyzer” Stabilized with Sodium Fluoride
James R. Shipe and John Savory
of Primary and Secondary Amines in Blood and Urine after Thin-Layer Joseph Monforte, Raymond J. Bath,
and Irving Sunshine
in Direct Determination
of Lactic Acid in Plasma
James 0. Westgard, Brenda L. Lahmeyer, and Marvin L. Birnbaum
1566
CLINICAL CHEMISTRY, Vol. 18, No. 12, 1972
November (continued) 1339
Radiolmmunoassay of Serum Renin Activity and Digoxin Concentrations with Use of Polyethylene Glycol to Separate Free and Antibody-Bound Ligand M. James Barrett and Patricia S. Cohen
1343
Gas-Liquid Chromatographic Isolation, identification, and Quantitation of Some Barbiturates, and Diphenylhydantoin in Whole Blood Rodney G. Cooper, May15 S. Greaves, and Goronwy Owen
1350
Use of 3-Methyl-2-Benzothiazolone Hydrazone in an Automated Calorimetric Triglycerides in Plasma or Serum
1355
Interlaboratory
Glutethimide,
Method for Determination
of
W. E. Neeley, G. E. Goldman, and C. A. Cupas and Intralaboratory
Comparison of T3-Uptake Methodology F. W. Spierto,
Ivey Lois Hubert,
and Robert
F. Witter
1360
Comparison of Rapid Clottable Fibrinogen Assays
1364
Automated Quantitative
1368
Haptoglobin Phenotype Frequencies in Mentally Retarded Persons A. T. Rundle, B. Clothier, and Barbara Sudell
1373
Serum Propylthiouracil:
1376
Column-Chromatographic Analysis of Naturally Fluorescing Compounds: 5-Hydroxyindoleacetic Acid in Biological Samples
Frederick
R. Davey,
Constance
E. Carrington,
and Douglas
A. Nelson
Immunochemical Analysis of Human Immunoglobulins Harold Markowitz and A. R. Tschida
Determination by a Direct Colorimetric Procedure Charles R. Ratliff, Paul F. Gilliland, and Frank F. Hall II.
Rapid Analysis of Indoleacetic Acid and
D. D. Chilcote
1379
Biological Applications of the Carbon Rod Atomizer in Atomic Absorption Spectroscopy. Copper in Small Samples of Tissue Brian J. Stevens
1385
Differentiation
1395
Quantitation
1403
Increased Activity of Some Enzymes in Serum in Cases of Severely Decompensated without Ketoacidosis
of Trypsin-Like
2.
Determination
of
Enzymes in Human Plasma Herbert D. Guilick
of Plasma Aldosterone by Radloimmunoassay M. J. St. Cyr, J. M. Sancho, and J. C. Melby
Francesco
Belfiore,
1407
Partial Correlation of Some Blood Constituents
1411
InvestigatIon
Elena
Napoli,
and Luigi
Diabetes, with and
Lo Vecchio
L. B. Roberts
of the Use of Calcein in the Ultramicro James
Fluorometric
F. Bandrowski
Determination
of Calcium in Serum
and Carl L. Benson
SCIENTIFIC NOTES 1415
Concentrations
of Lead in Capillary Blood of Newborns Norman P. Kubasik and Michael T. Volosin
1416
Automated Hexokinase Procedure for Assaying Glucose in Urine, Serum, or Plasma Hugh Y. Yee
1419
Simple, Rapid, Kinetic Method for Serum Creatinine
Measurement
Jack A. Lustgarten and Robert E. Wenk 1423
Effect of Hemolysis on the Apparent Concentration of Insulin in Plasma JeraId W. Cantrell, Jane M. Hochholzer,
1425
Spot Applicator for Quantitative
1427
Significance of Increased Alkaline Phosphatase and Lactate Dehydrogenase Activities Coincident with Normal Serum Bilirubin
1430
Modified “Calcium Base Reagent” for Simultaneous Phosphate with the “AutoAnalyzer”
and Christopher
S. Frings
Thin-Layer and Paper Chromatography, Suitable for Clinical Analysis Arthur F. Rosenthal and Marta G. Vargas
M. G. Betro
J. FischI
1433
and
Determination
of Serum Calcium and Inorganic
F. Schwartz
THE CLINICAL CHEMIST J. S. King, Jr.
1438
BOOK REVIEWS N. Radin,
Robert
F. Labb#{233}, Ian H. Carlson,
and
Lena A. Lewis
LETTERS
1440
Preparation
and Use of a Urine Control for Certain Drugs of Abuse Christopher
1440
Speeded Simultaneous
Turbidimetry
Sickle Cell Anemia:
and Cecelia
A. Queen
of Cholesterol in 9- and Pre-p-Lipoproteins L. Gremillion,
1441
S. Frings
L. Bell, and A. Lopez.S.
A Suggested Screening Procedure William F. Vincent, William H. Harris, and Bernard Rosenberg CLINICAL
CHEMISTRY, Vol. 18, No. 12, 1972
1567
November (continued) 1442
Transaminase
Activities in Serum of Long-Term Hemodialysis Patients D. W. Bradley, J. E. Maynard, G. Emery, and H. Webster
1442
Stability of Certain Drugs of Abuse in Urine Specimens Christopher S. Frings and Cecelia A. Queen
1442
Oxytocinase
1443
Effect of Hydrogen Ion Concentration on Lactate Dehydrogenase (LD) Assays John King
1443
Prevention of Hemolysis-Induced
Activity
Determination:
A Modification J. Peeters
Interference G. Wien
1443
Names and Symbols for Quantities
1444
Improved BUN Measurement
with Digoxin Assays
and R. Kumar
Ole Siggaard.Andersen
Method Orville R. Sapp
December 1445
REVIEW: Pathophysiological
Aspects of Catecholamine
Production
U. S. von Euler
1449
GUEST EDITORIAL: The Relative Merits and Applicability of Kinetic and Fixed-Incubation Methods of Enzyme Assay in Clinical Ezymology D. W. Moss
1455
Direct Potentiometric
Determination
of Total Ionic Fluoride in Biological Fluids Larry L. Hall, Frank A. Smith,
1459
Measurement
Ofelia
H. De Lopez,
M. M. Popovtzer,
S. D. Parks,
1463
Simplified
1468
Serum Alkaline Phosphatase: Total Activity and lsoenzyme Determinations Made by Use of the Centrifugal Fast Analyzer Manual Spectrophotometric
E. Statland,
and Fluorometric
Nishi,
and D. S. Young
of Tocopherol in Cerebrospinal
Effect of Aging of Serum on Quantitation
1488
Rapid Method for Determining
1493
Isolation and Measurement
Jr.
of Complement Component C3 C. D. West,
and M. Ho
Ultrafiltrable Calcium in Serum Bent Halver
of Pancreatic Amylase in Human Serum and Urine L. Fridhandler,
J. Edward
Berk,
and M. Ueda
Studies of the Stability of 18 Chemical Constituents of Human Serum Sherman
S. Wilson,
Ramon
A. Guillan,
and E. Virginia
Hocker
1504
Statistics of Patient Test Values: Application to Indirect Normal Range and to Quality Control
1514
Simple Automated Spectrophotometric
John
H. Glick,
Jr.
Method for Assay of Trypsin and Chymotrypsin in Duodenal Juice A. Vandermeers,
1518
Automated Method for Determination
J. Rath#{233}, and J. Christophe
Immunochemical
F. Smith G. Bethune,
A. Fogg
Martin
Fleisher,
and Morton K. Schwartz
Quantitation of Prostatic Phosphatase Vita Milisauskas
SCIENTIFIC
and Barbara
of Serum 5’-Nucleotidase Activity Victor
1529
M..C. Vandermeers-Piret,
Possible Mechanisms for the Increase in Alkaline Phosphatase Activity of Lyophilized Control Material Alistair
1524
Fluid
and G. A. Mortenson
‘3Ni Complexes in Rabbit Serum and Urine after Injection of ‘3NiCI2 Maria Van Soestbergen and F. William Sunderman, N. C. Davis,
1498
H. Harold
Determination
G. T. Vatassery
1485
and E. B. Reeve
Primary Screening Procedure for Detection of Hyperlipidemias in Healthy individuals Gerald S. Berenson, S. R. Srinivasan, P. S. Pargaonkar, B. Radhakrishnamurthy, and Edward R. Dalferes, Jr.
Bernard
1478
E. Gardner
of Serum Ionized Calcium with the Ion-Exchange Electrode V. L. Subryan,
1475
and Dwight
and
Noel R. Rose
NOTES
1532
Inhibition of Leukocyte Acid Phosphatase by Heparin
1534
Simple Method for Determination
1537
Effects of Various Medications on Values from the HABA and BCG Methods for Determining Albumin Solomon Notrica, Don S. Miyada, Vern Baysinger, and Robert M. Nakamura Aspartate Aminotransferase (“SGOT”) Assay in Serum, with Use of the AutoAnalyzer II 340 Colorimeter in a Conventional AutoAnalyzer I System, Including Dialysis
Lawrence
R. DeChatelet,
Katsumaro
1539
Arthur
1541
Estimation of Serum Proteins by Electrophoresis
CLINICAL
CHEMISTRY,
Vol. 18, No. 12, 1972
and Masana
L. Levy and Susanna
J. W. Keyser 1568
Tomokuni
E. McCall, M. Robert Cooper, and Pamela Acid as an Index of Lead Exposure
Charles
of Urinary -Aminolevulinic
Ogata
Konig.Levy
on Cellulose Acetate and G. L. Watkins
S. Shirley
December(continued) 1544
THE CLINICAL CHEMIST
J. S. King, Jr. LETTERS 1547
Extraction of Unconjugated
Billrubin from Serum Anna Bell Ham
1547
On Iodine Calibration
1549
A Calculated Adjustment of the Baseline in PBI AutoAnalyzer Runs J. C. MacLaurin, B. Rundstedler,
Curves Henry Fine-Man Chung, J C. MacLaurin,
and B. Rundstedler
and Henry Fine-Man Chung
1549
Urea Therapy of Sickle Cell Anemia
1550
Methods for Vitamin B12Assay: Some Comments Eugene P. Frenkel and Jerry D. White
1550
Furfural:
1551
Validity of the Lecithin/Sphingomyelln
1552
Modification
Robert M. Nalbandian
Exogenous Precursor of Certain Urinary Furans and Possible Toxicologic Agent in Humans Eugene
W. Rice
(L/S) Ratio for Amniotic Fluid Containing Blood Rita Bryan
in a Screening Test for Triosephosphate
Isomerase Deficiency
Mei Lee Lowe and Jerry B. Gin
1552
Blood Acid-Base Values Calculated with Desk-Top Computers Judith
1552
TLC of Porphyrins
1553
INVITED REVIEWERS, 1972
1554
BOOK
Ann Thomson
and William
S. Medart,
Jr.
Joel Lamon
REVIEWS H.-D. Gruemer, Paul B. Hamilton, Charles L. Woronick, Reimut Wette and Mario Werner, and D. S. Young
ANNUAL INDEX, Volume 18, 1972 1558
Contents
1570
Author
1571
Subject
CLINICAL
CHEMISTRY,
Vol. 18, No. 12, 1972
1569
