Angelo Burlina. 321 DigitalisGlycosldes in .... Angelo A.Lamola. 414 Taurlne Transport In ..... Hemoglobins. Andrea Mosca,Angelo Carenini, Michele Sama-.
January
Volume 26 Numbers 1 to 13
1980 to December 1980
llfifill
pSSOc,4
j#{149} 5c.IIc,
Journal of the American
JANUARY
for Clinical
Chemistry
69 Spectrophotometrlc Screening Methodfor Acetamlnophen In Serum and Plasma TsanZ. Liu and Kris H. Oka 72 SimplIfyingLymphocyte Cufture and the Deoxy-
1 InformatIonfor Authors 5 REVIEW: Measurement of TricyclicAntidepressants. Part L A Review of Methodology Bruce A. Scoggins, KayP. Maguire. TrevorR. Norman, and aham 0. Burrows
18 Advantages of Using Thin Sodium Iodide Detectors for ThyroidMonitoringof Personnel Working wIth ‘I Susan Landon, P. G.Smith,
Association
urldine Suppression Test by Using Whole Blood (0.1ml) instead of Separated Lymphocytes Kshitish C. Das, Cathy
Manusselis, and Victor
Heshert 78 Metaslable Creatine lClnaseMMand Complexes of Creatine Khsase B8 and lmmunoglobullnC, “Atypical” Isoenzymes with SimIlarElectrophoretic Mobility
S.P. Staniek, and C.
E. Webber 22 MechanIsmof Interference by HemoglobinIn the Determination of TotalBilinibin.L Methodof Malloy-Evelyn Bruce C. Shull, Helen Lees,andPhilip K. Li 26 MechanIsmof Interference by HemoglobinIn the Determination of Total BIlirubin. IL Method of Jen*assik-Gmi Bruce C. Shull, Helen Lees, and Philip K. Li 30 Biochemical Individualityand the Recognition of Personal Profileswith a Computer E. Arthur Robertson, An&e C. Van Steirteghem, James E. Byrkit,and DonaldS. Young 37 SolId-Phase Radlolmmunoassay with ProteinA-Bearing SIa,ylococcus aweus Cells Used to Assay a Protein (Ferritln) and a Hapten (Dl-
goxin)
Judith N. Rash, lvanka Jericunica, and Demetrios S. Sgoutas 89 ImmobIlIzed-EnzymeAate-Determlnatlon Method br Glucose Analysis LuciaSolcol, Carl Garter, Mark Shults, and Stuart Updike 93 A Fluorometrlc Determination of Sphingomyellnase by Use of Fluorescent DerIvatives of Sphlngomyelln, and Its Applicationto Diagnosis of Nlemann-PIck Disease $. Gab, T. Dinur, and V. Barenholz 97 A Fully Automated, Continuous-Flow Radlolmmunoassay br Methotrexate Reyad Kamel, John Landon, and GordoC. Forrest
J. Gauldie,H. K. Tang, A. Corsini, and W. H. C. Walker 41 ScreenIng Radlommunoassay for Aldosterone in Preheated Plasma wIthout Extraction and Ctwo-
matopaphy
ThomasN. Connolly,LaszloTibor, Kurl-Holger Gless,andPaul Vecsei 46 MaxImIzingthe Efficiencyof an AdmissionsTest ProfileIn a 680-BedHospital C. WilliamCole 51 “HIgh-Pressure” LiquidChromatography of Sulfisoxazole and N’.Acetylsulflsoxazole In Body Fluids Donald JungandSveinGie 55 CIrculating CK.MBand CK-BB Iso.nzymes
HenryA.Hombctger, LesterWold,GregL. Jacob, and John O’Brien $4 LipidInterferencehi Steroid Radlolmmunoassay
alter
Prostate Resection
Stephen C. Kimlerand Randoir S. Sanchi 60 VarIation of Carbonic Acid pl(’ip In Blood and Urine during NaHCO3 Infusion and NH4CI Loading: A Study of Two Renal Acidotlc Patients GerardJ. van Stekelenbttg, Coos Valk, and RaymondA.N.G.Donckerwolcke 86 ExtractIon and Determination of Chloroform in Rat Blood and Tissues by Gas ChromatographyElectron-Capture Detection: Distritiutlon of Chloroform hi the AnimalBody CorazonR. Vogt, John C. Liao, and Albert V.
101 Evaluation and Clinical Application of a Dexfran-Charcoal Method for Measuring Bound Testosterone in Human Plasma Robert K. Tcholalcian,GeraldN. Jones,Barbara N. Sanborn,Luis J. Ro&iguez-Rigau, and Gerald G. Reimondo
107 BlIlnibin Binding
by Primary Sites of Human Serum Albumin,with Suppression of SecondarySite Interference by Use of a HighSalt Concen-
tration
RomanB. Rutkowski 111 Simultaneous Llquid-ChromatographlcQuantitalion of Sallcycllc Acid, Sallcylurlc Acid, and GentisicAcid In Urine B. Elliot Cham,Felix Boctiner,DebraN. Imboff, Denise Johns,andMalcolm Rowland 115 ImmunoglobullnsIn Cerebrosplnal Fluid In VarIous Neurologic Disorders A.Bouloiicos, J.Lelcaicis,J. Michael,andA. Kalofoutis
117 Llquid-ChromatographlcDetermination of 5-Fluorocytosine John 0. Niners, Thimy Foenander, and DonaldJ. Birkett 120 EvaluatIonof a Radloenzymlc Kit forDeterminatimofPlasma Catecholamines Sara J. A. Tasseron,Jan W. T. Fiolet, and Albertus F. Willebrands
123 Contlnuous-FiowAnalysis for Glucose in Serum, with Use of Hexokinase and Glucose-6-Phosphate Dehydrogenase Co-ImmobIlIzedIn Tubular Form Luis P. Leon, Douglas K. Chu, Lloyd R. Snyder, and CsabaHorv#{226}th
SCIENTIFIC NOTES 130 DetermInatIon of Urinary Placental Estriol by Reversed-Phase Liquid Chromatography with
Fluorescence Detection JamesT. Taylor, J. G. Knobs, and C.J. Schmidt 133 The Momlayered Film Analyzer: Glucose in Serum, Plasma, Cerebrosplnal Fluid and Urine; and Urea Nitrogen in Serum and Plasma Karen Warren, NormanP. Kubasik,BernardB. Brody. HarrisonE. Sine, andJohnP.D’Souza 138 Response of Ion-SelectIve Sodium and Potassium Electrodes in theBeckman Astra 4 and Astra 8 Analyzers P. Fievet, A. Truchaud,J. Hersant, and G. Glikmanas 139 Parallel Evaluation of Astra 8 andAstra 4 MultIchannel Analyzers in Two Hospital LaboratorIes A. Truchaud,J. Hersant,G. Glikmanas,P.Fievet,
and0. Dubois 142 MonitorIng Clorazepate
Dlpotaulum as Desmethyldlazepam In Plasma byElectron-Capture Gas-Liquid Chromatography Dan HaidiJiewych,ErnstA. Rodin,and Ronald Davenport
145 Immunoradlometrlc Serum Ferrftln Concentration Compared with StainableBone-MarrowIron
as Indices to IronStores DonaldT. Forman andMalcolmV. Vye 147 Measurementof Sodium Vaiproate in Serum by DIrect-insertIon Chemlcal-Ionlzatlon/Mau Spectrometry Gary MartinSchier, Ignatius Eng Tho Can, Berthold Halpern, and JohnKorth 150 Clinical Evaluation ofan ImmunolnhlbltlonProcedure for Creatlne Klnase-MB DavidObzanslcyand John A. Lob 153 Improved Radlochemlcal Method for Measuring Ferrochelatase Activity H.L. Williams,D.J. Johnson, N. J. Haut. S. Slater, R. G. Brennan, and L. B. Altstatt 156 Use of An Alumina Column in Estimating Total
Iron-BindingCapacity RossT. Starr 159 An Improved UltrafIltratIon MethodforFree Thyroxine and Trllodothyronlne in Serum Judy Sophianopoulos,lvanka Jerkunica, Chieri Ning Lee, and DemetriosSgoutas
CASE REPORT 163 Drug-Induced Dysllpoprotelnemla: A Report of Two Cases
CLINICAL CHEMISTRY, Vol. 26, No. 13. 1980
1935
Herbert K. Naito, Martin C. McHenry, and LenaA.
214 Radlolmmunoassay for Detection of Latent
Lewis
ChronIcAlcoholicPancreatls, an Unrecognized
LETTERS
Clinical Syndrome
169 HDI. Cholesterol: Results of Interlaboratory Proficlencey Tests C. Russell Warnick, JohnJ. Albers, and Elizabeth Teng Leary
170 Assessment of New UrinalysisDipstick CraigR. Hearne,Nichelle G. Donnell, and Callum G. Fraser 171 Cause of Alkalosis in “Diabetic Ketoalkalosls” Dennis St. J. O’Reilly and JohnP. Delamere 172 SImultaneous Testing for Glucose and TOtalRe-
ducing Substances
in
Routine Urinalysis
Joseph L. Potter
173 Evaluation of a Thronibln-ContainlngBlood-Collection Tube Steven J. Steindel 174 Glycosylated Hemoglobins: Hematologlc Considerations DetermIne Which Assay for Glycohemoglobin Is Advisable RalphE. Bernstein 175 CollectIon and Storage of Serum LactIc Acid Samples at Room Temperature without Deprotelnizatlon Lawrence A. Kaplan,Nancy Gau. and Evan A.
Samuel T. Nerenberg, Rameshwar Prasad, Linda
DeMarcoPedersen, and NancyS.Biskup 219 Personal andFamIlial Factors InCholesterolemIa: Criteria for Selection of a Reference Population Josiane
Steinmetz,
Edwige Panek, and Gerard
Siest 227 Use of 3,5-Dichloro-2.hydroxybenzenesulfonic Acid/4-Amlnophenazone Chromogenic System In Direct Enzymlc Assay of UricAcid in Serum and Urine Piero Fossati, Lorenzo Prencipe, and Giovanni
297 BInding of Enzyme-lgG Complexesin Human Serum to Protein-A Sepharose CL-4B Kurt Bauer, Peter N. Bayer, Erwin Deutsch, and Franz Gabi 301 Effect of Gestational Variationof Phosphatldylglycerol andPhosphatldyllnosltol onthe Microviscosity of AmnioticFluidPhosphollplds Howard S. Cheslcin,ThomasA. Blumenfeld, Philip C. Beers,and Venkat Buddega 305 A Lyophilized Hemoglobin Control Prepared from Stroma-Free Hemolysates D.P.Bonderman,G.J.Proksch.and S.Haskins
SCIENTIFIC NOTES
Berti 232 Effects of Centrlfugatlon, Storage, andContaminationof AmnioticFluid on its Total Phospholipid
Content Eveline J.C. N. van VoorstlotVoorst 235 ImmunologIcal, Biochemical, and Enzymlc Validationof RadloimmunoassaysSpecific to the
Amino and CarboxyTerminalof HumanCalcItonin William C. Dermody, Regina Ananthaswamy,
Mark A. Rosen, Fulvio Perini, and Amalia C. Levy Stein 243 HepatlcFunctionIn Mononucleosis Inducedby 176 Electrolmmunoassay of Apollpoproteln A-I on Epstein-Barr Virus and Cytomegalovirus Mylar-SupportedCellulosic Membranes CharlesA. Horwitz, M. Desmond Burke, Patricia C. F. Hosler, Jr. Gomes, and JosephTombers 177 Quality Control in Assay of HIph.Denslty Upo247 SpecIfic Lymphocyte Phosphollpid Changesin protein Cholesterol ChronicRenal Failure Wolfgang Hohenwaliner, Rudolf Sommer, Ernst Anastasios Kalofoutis Wimmer. andGertraudV. Tschurtschenthaler 178 Aldehyde Dehydrogenase interference in the 250 Amylolysls of a Chromogenic Substrate, CibaHaeckel Enzymlc TechniqueforUric Acid Dechron Blue F3GA.Amylose: Kinetics and Mechatermination nism Francesco Zoppi, Domenico Fenili, Santica MarBernard Klein and James A. Foreman covina,and An&ea Peracino 254 Rate of Decrease of Glutamyitransferase and ACIdPhosphatase ActIvitiesIn the Human VagiBOOK REVIEWS na after Coitus 179 Michael N. Lubran, Elizabeth B. Solow,and W. WadieF.Gohara E. Cornatzer 258 Improved Electrothermal Determinationof Alu181 THE CLINICAL CHEMIST minum In Serum by Atomic AbsorptionSpecJ. S.King troscopy Frances R. Alderman and Hillel J. Gitelman 261 3-HydroxydecanedlolcAcid and Related HomolFEBRUARY ogues: UrinaryMetabolitesin Ketoacidosis Joachim eter, Sven Lindstedt, Helene Seeman, and Goran Steen 185 REVIEW: Nutritional Importance of Copper and 266 Evaluationof an Engineering Model of the “EKZinc In Neonates and Infants TACHEM” Analyzer for Glucose and Urea Philip A. Walravens Assay 190 SemI-AutomatedFluorometryof Total Estrogens JohnC. Cab, IV,Regina Hedrick, Malcolm Taylor, In Plasma during Late Pregnancy and C. David McGlothlin Ctwistiane Goutte-Coussieu, Daniele Sanchez, 271 Whole-Bloodand Plasma Amino Acid Analysis: GerardAdessi,and Max-PernandJayle Gas-Liquidand Catlon.ExchangeChromatogra194 Liquld-Chromatographic Determination ofNorphy Compared eplnephrine and Eplnephrine in Human Plasma Anne M. Lewis, Christine Waterhouse, and LauYoshiki Yui, Tomio Fujita, Tamotsu Yamamoto, rence S. Jacobs YoshinoriItokawa, and Chuichi Kawai 277 AmniotIc Fluid Phosphatidylglycerol and Phos197 SImultaneous Liquld-Chromatographlc Determiphatldyllnosltol Separated by Stepwise-Develnation of Three Antiarrhythmlc Drugs: Dlsopyopment Thin-Layer Chromatography ramide, Lidocalne, and Qulnidine MaryAnn Mitnick, Bryan DeMarco, and John M. JamesG. Flood, GeorgeN. Bowers,andRobert B. Gibbons NcComb 282 Continuous-Flow Fluorometry of Low Galactose 201 PatternofUrinaryProteins and PeptldesIn PaConcentrationsIn Blood or Plasma tients with Rheumatoid Arthritis InvestIgated J. MichaeLHendersonand Frank W. Fales with the lso.DaItTechnique 286 Development and Validation ofa Liquld-ChroPenelope N. S. Clark, Larry J. Kricka,andThommatographic Procedure for Serum Creatinine F. W. Spierto, N. L. MacNeil,P. Culbreth, I. Dunas P. Whitehead 205 SImultaneousLIquld-Chromatographlc Determican, and Carl A. Burtis nation of Zinc Protoporphyrln IX, Protoporphyrin 291 SImultaneous LIquld-Chiomatographlc Analysis IX, and CoproporphyrinIn Whole Blood for 4.Hydroxy-4-methoxymandellc Acid and 4George R. Gotelli, Jeffrey H. Wall, Pokar M. Hydroxy-3-methoxyphenylacetlc Acid in Urine Kabra, and LaurenceJ. Marton Steven J. Soldin and J. Gilbert Hill 209 IsolationandCharacterization ofa Human Pan295 Gas-Chromatographlc Determination ofAmantacreas-SpecIfIc Protein dine In HumanUrine S. T. Nerenberg, R. Prasad,L. Pedersen,N. S. MaryJane Stumph, MatthewW.Noall,and Vernon Biskup, and I. Faiferman Knight
1936 CLINICALCHEMISTRY,Vol. 26, No. 13, 1980
309 Two-Hour Sephadex ColumnMethod forAssay of Unconjugated Estriol InSerum W. Geoffrey Naigh, Lindsay F. Hofman, and Edward J. Barron 312 Improved ProteIn-BIndIng Radloassay for Plas-
ma Cortlsol Flana Novacenko, Frieda Halpern, and Edward J.
Sachar
314 Comparison
of Radial Immunodlffuslon and
Laser Nephelometry for Quantitating Some Serum Proteins Roy L. Alexander, Jr. 317 ImprovedElectrophoretic Separation of Creatine Kinase lsoenzymes Angelo Burlina 321 DigitalisGlycosldes in Serum,Urine,and CarebrospinalFluid,Determined with a Commercial Radioimmunoassay Anne K. Nore,Liv Storstein, Anne Larsen,andJan P. Amlie 323 Room Temperature Radioassay for B12 with Oyster Toadflsh (Opsanus tau) Serum as
Binder Dionyssis S. lthakissios, David0. Kubiatowicz, and James H. Wicks 327 Improved Direct SpecifIc Determination of
SerumIron andTotalIron-BindingCapacity Ferruccio Ceriotti and Giovanni Ceriotti
331 AutomatedMultipleFlow-Injection AnalysisIn Clinical Chemistry: Determination of AlbumIn with Bromcresol Green B. W. Renoe, K. K. Stewart, C.R. Beecher, N.R. Wills, and J.Savory 335 Enzymlc Inhibition Assay forMethotrexate with a Discrete Analyzer, the ABA-100 Linda F. Brown, George F. Johnson, David L. Wine, and Ronald D. Fold 339 EvaluatIon ofan ImmobilIzed-EnzymeAnalyzer for Measuring Galactose In Serum Gregory J. Buffone, Jay N. Johnson, Susan A. Lewis,and JohnW. Sparks 341 AdaptatIonof the Enzyme-Multiplied Immunoassay for Methotrexateto the Centrifugal Ana-
lyzer Paul R. Finley, A. Jane Williams, Frank Griffith, and D. A. Lichti 343 Two Agarose Electrophoretlc Systems for Demonstrationof Oligoclonal Bands In Cerebrosplnal FluidCompared Benjamin Gerson, F. John Krolikowski, and Irvin M. Gerson 345 False-NegatIve Results for Urinary Phenothiazines and Imipramine in Forrest’s Qualitative
Assays Gordon P. James, Mary Helen DJang, and Horace H. Hamilton
LETTERS 348 Increased Circulating Creatine Klnase Isoenzyme BB in a Patientwith Metastic Prostatic CarcinomaGave Spuriously HIgh IsoenzymeMB Values with the Harleco UltraZyme Kit JohnEckfeldtand Marcia Kershaw
349
MARCH
CritIcismof theProposed Selected Method: Direct Measurement of ZincIn Plasmaby Atomic
463 Thyroxlns-Ritdng Globulin Capacity and Con-
centratIon Evaluated from BloodSpots on FIlterPaper In a Screening Programfor NeonatalNy-
Absorption Spectroscopy James R. Kelson
(Reply) J. Cecil Smith, Jr., Gerald P. Butrimovitz, and William C. Purdy 351 Estimationof the Specific Activity of RadiolabeledCortlcotropln andInsulinby Precipitation ExequielA. Virasoro,LauraE. Quihillat,and Oscar0. Bruno 352 Test DataMatrix andResultsfor Linear Regression Analysis by Methodof W.E. Deming Roy F. Schall, Jr., Carl W. Kern, and Hal J. Tanoso
352 Ferrltin, Transferrln, and
Iron: Relations
In
Serum Horst D. Koehn, G#{252}nter Wider, PeterM. Bayer, and Adolf Mostbeck 353 lgD Myeloma or 5 Heavy-Chain Disease? Adrian0. Vladutiu 354 Is Serum or HeparinizedPlasma Betterfor
Assay of Aminotransferases?
369 OPINION: IFCC Documents and Interpretation of SI Units-An AdaptableSolution A.Dybkaer, J. C. Rigg,and A. Zender
371 REVIEW: Acid Phosphatase In Gaucher’s
Dis-
ease
473 ConditIonsAffecting the Colorlm.try of Orotic
DouglasB. RobinsonandRobertH. Clew
383 OntheUs. of StatIstical Models of Within-Person VarIationInLong-Term Studies of Healthy IndivIduals Eugene K. Harris,BruceK. Cooil,GeorgeShaker-
ji, and George Z. Williams 392 IncIdence of a Split a2-Glycoprot.in Band In the Electrophoretic Pattern for Serum of AdenocarclnomaPatients Gerald B. Dermer, Lawrence N. Silverman, San&a J. Gendler. and Zoltan A. TokOs 396 QualitatIve Testing for CirculatingImmuneComplexes by Use of Zone ElectrOphoresIs on
J. C. N. Hafkenstheid and C. C. M. Dijt 355 CorrectIons to the Amlnoglycoside Review Agerose Rejean Daigneault, Alain Larouche, and Ga#{233}tan R. H. Kelly, Margot A. Scholl, V. Susan Harvey. Thibault and A. G. Devenyi 355 DetectIon of AbnormalHemoglobinVariants 403 AmniotIcFluidPhospholipldsMeasuredby Conduring GlycohemogloblnAnalysis tlnuous-Development Thin-Layer ChromatogCarol A. Lapp. Thomas A. Heft, and Edwin D. raphy Bransome,Jr. Mark D. Kolins. Emanuel Epstein, W. HaroldCivin. 356 False-PosItive Solubillty-Turbidity Testfor Sickland Sheldon Weiner ing Hemoglobinin a Case of Congenital Syph- 406 Radlolmmunoauay of Thyroxine in 10 MicrofIilis tars of Serum, with Us. of Aggregated AntlthyJulie P. Mann and Antranik A. Bedros roxlne Antibodies 357 A Cheerful Reporton SI UnitAcceptance Sonia Collins, Marion Brooks, andEdwardW. J. 1. Hindmarsh, C. L. Cousins,and W. J. LonBermes gley 409 HemoglobinDeterminedIn15sL ofWholeBlood 357 SafeUse of Phenazine Methosulf ate by “Front-Face”Fluorometry HenryJ. Gartield WilliamE. Blumberg, Franklin H. Doleiden, and 358 Preparation ofReagents forUsewith the BeckAngelo A.Lamola man Astra-8 414 Taurlne Transport In Epilepsy John H. Click, Jr., Doris M. Brown, Carolyn L. Harold0. Goodman,BrianN. Connolly, William Crocker, Sheila M. Halsten, and Cynthia A. Peine McLean, and MartinResnick 359 Potential Bilirubin Interferencewith the Techni420 ImmunolOglcal and Biochemical Evidence for con SMAC Alkaline PhosphataseActivity MeaIdentity of Tartrate-Resistant Isoenzymes of surement Acid Phosphatases from Human Serum and Karen J. Whitlow, NathanGochman,and Alfred Zettner Kwok-Wai Lam, Paul Lee,Chin-Yang Li, and Lung 360 Problems with ‘25l-LabeledTyrosyl Ligands In T. Yam Digoxin RIA 423 Effectsof MetalIonsonthe Measurement of AlBruce H. Kroening and Michael Weintraub kaline Phosphatase Activity 360 Glycosylated HemoglobinsIn Patients with a RobertRejand Jean-PierreBretaudiere Hemoglobinopathy 429 SimplifIedRadlolmmunoassay of Bradykinin In N. D. Fitzgerald and N. N. Cauchi Human Plasma 361 RenalDiseaseMayIncreaseApparent Phenytoln Pritam S. Verma,Patrick E. Lorenz, and Gary E. in SerumasMeasuredbyEnzyme-Multiplied ImSander munoassay 433 Prostate-SpecIfic AcidPhosphatase: Re-EvaluaHelgaFlachs and JorgenMelchiorRasmussen; tion of Radioimmunoassay in Diagnosing ProsDonnaN. McDonaldandPokarN. Kabra tatic Disease (Reply) John C. Griffiths Kenneth Rubenstein and Richard Bastiani 437 Gas-Chromatographlc Analysis for Phencycli362 SensItivity of the IL282Cooximeter to Low Hedine In Plasma, with Us of a Nitrogen Detector moglobin Concentrationand High Proportions of David N. Bailey and John J. Guba Methemoglobln 441 MannltolEstimation In Biological Fluids byGasH. L. Sehgal, L. A. Sehgal, A. L. Rosen, R. LiquidChromatography of Trlmethylsilyl DerIvaDeWoskin,S.Gould,andC. S.MOSS tIves 363 IsolatIonof6-Demethylgriseofulvin from Urine N. F. LakerandJ. N. Mount Randall S. Wagner, Bruce G.#{149}Charles, and John L. 444 A SImplIfiedAssayforDlhy*oxylatedVitaminD Lach Metaboiltes In Human Serum:ApplIcatIonto
BOOK REVIEWS 364 Alex Kaplan, Larry I. Galblum, Mario Werner, H.-D. Gruemer, RobertJ. Mohrbacher, and Elvin A. Kabat
THE CLINICAL CHEMIST 367 J. S. King
Hyper- and Hypovftamlnosl. D Rebecca S. Mason,Dianne Lissner. Harry S. Grunstein, and Solomon Posen 451 AlkalIne Phoiphatase of High and Low Molecular Mass In Human Serum and Bile A Comparative Study of Kinetic Properties PatriciaN. CroftonandA. F. Smith 457 Further HeterogeneIty Demonstrated for Serum Creatlne Kinase lsosnzyme MM Jean-Paul Chapelle and Camille Heusghem
pothyroidism Jean H. Dussault,JeanMorissette,Jacques Latarte,Harvey Guyda, and Claude Laberge 466 Accuracy, Precision,and StabilityIn Measurement of Hemoglobin A1 by “High-Performance” Cation-Exchange Chromatography Richard S.Schifreen, J.MarkHickingbotham, and George N. Bowers. Jr.
AcidandOrotIdin.
In Urine
Margaret1. HarrisandV. C. Oberholzer 480 An InterlaberatoryStudy ofthe Determination of Digoxinby Immunoassay DouglasN. Fast,W.HarryHannon,CarlA. Burtis, and DavidD. Bayse
SCIENTIFIC NOTES 487 NonspecIfic Binding as a Sourc, ofErrorin Thyrotrepin Radiolnununoassay with Polyethylene Glycol as SeparatIng Agent I-Won Chen, L. Heminger,H. R.Maxon,andJ.Y. Tsay 491 EnzyinIcColor Developmentof Urinary 3a-Hy-
oxysteroIds on Thin-LayerClwomatograms YoshthisaYamaguchi 493 ContInuous-Flow(AutoAnalyzer I) Analysis for Plasma Thlocyanate as an Index to Tobacco Smoking EdwardR. Dalferes, Jr., Larry S.Webber, Bhandaru Radhakrishnamuthy, andGeraldS. Berenson 496 BindIng of IgGandOtherProteinsto Microfilters R.L. WalshandM.E.Cobs 499 Measurement of Antlconvulsants In Serum by Reversed-PhaseIon-PaIr Liquid Chromatography John A. Clwistofidesand Denys E. Fry 501 S.mlautomated Homogeneous Enzyme Immunousay of TotalSerumThyroxinewith a KinetIc
Analyzer ilelle Bhatia 503 Pre-Preclpltated and SolId-PhaseSecondAntIbodyCompared In Radlolmmunoassays ThomasA. Brown.NandalalBagchi,ThompsonT. S.Ho,andRobert E. Mack 508 PotentIal Sourcesof Errors In Cation-Exchange Chromatographlc Measurementof PlasmaTaurine
BrianN. Connolly and Harold0. Goodman
CASE REPORT 511 TransIent
Increase In Serum Creatine Klnase lsoenzym. B8 ActIvIty after ProstatectomyIn a Patient with MassiveBenign Prostatic Hyperpiasia ZacharyD. GoodmanandStanleyR. Hamilton
SPECIAL REPORT 513 An Interlaboratory Study of Creatine Klnase and
Cr.atlne Kinase Isoenzymes Joe Boone,E. J. Sampson,S. Lewis,V. Whitner, S. McKneally. and B. Houston
LETTERS 520 Drift in Sasac-DeterminedSerum Creatinine Can Be Reduced byAddingEDTAto the Creatinine Recipient Solution Ctwister Tagessonand Karl Rebel 520 Lack of RelationshIp between Mg24 and K
Concentrations in Serum Kenneth A. Watson and Richard T. O’Kell
521 ExtractIon Procedure for MeasurIng Anticonvulsant DrugsbyUquldChromatography
CLINICALCHEMISTRY,Vol. 26, No. 13, 1980
1937
SCIENTIFIC NOTES
APRIL
Samuel V. Chu,L. Oliveras,andS. Deyasl 522 Erythrocyte, Plasma, and Semen FofMe Speci-
menSlablity beforeMk.oblo’OgIcalAssay J. D. 0’Broin, I. J. Temperley.andJ. N.Scott 524 SeparatIon andQuaniltatlen of Theopltylln. and Paraxanthine by Rsv.rsed-Phas. Uquld Quomatography Herbert14.Farrish andWilliamA. Wargin 525 Alcohol Dehydrogenase inted.rence In Lactate Dshy*ogenass Assay Renze BailsandJohnB. Edwards 526 Glycosylatlonof HemoglobinS and Hemoglobin
C H. Aleyassine 527 Slmplifled Colorimetry ofUrinary Homovanillic
Acid J. L. Barronand B. A. Peak 527 ElImInatIon of Pyruvate Interference In the En-
zymic DeterminatIonof Amylase Viliam Lustig
629 Performance of a Radiometer
Transcutaneous Oxygen Monitor In a Neonatal-Intensive-Care
Unit GaryGraham andMargaret A. Kenny 633 MicroencapsulatedAntibodies in Radloimmunoassay.III. Determination of Cortisol Ronald W. Bordensand E. Philip Halpern
543 REVIEW: Methodsfor Ligand-Receptor in ClinicalChemistry Roy G. Smith and Mary Ann Sestili
551 Kinetic SerumCreatlnineAssaysI. TheRole of
Evaluation of a Kit forMeasuring Tricyclic AntIdepressants Kay P. Maguire,ahan D. Burrows,TrevorR. Norman,andBruceA. Scoggins Reply George H. Wien 530 MIsstatementsin a Paper PaulA. Finley 530 CompressIon of Sample-LineAir Segments-A Source of Error hi the Tecfmicon SMAC 529
System 1. A. Walmsley,
N.
H.
Abernethy,andR. 1.
Fowler
531 DetermInation of Manganesein WholeBloodand Serum Jacques Versieck, Rita Cornelis,eta Lemey, and Julien De Rudder 532 BedsIdeEstimationof PlasmaLactate Fran#{231}ois Piquard, A&ien Schaefer,PascalHaberey, and Pierre Dellenbach 533 ScreenIng for Polymeric1gM:Some Overlooked
History G. Virella 534 Mere NearlySpecificFluorometryof TheophyllIne in Serum John M. Meola, Thomas Swift, and HaroldH. Brown 534 Comments on a CaseReportonMyocardlal Infarction Charles J. Przyjemski; also Sunny S. Fike and Eric Frow
Reply WilliamBorer,JohnGottdiener,andNickPapadopoulos 535 Total Creatinine Content of the First Morning Urine Is Independent of DietaryChange P. W. Banda, N. S.Tuttle, A. E. Sherry, and N. S.
Blois
537 BOOK REVIEW J. S. King
539
THE CLINICAL CHEMIST J. S. King
Evaluationand Review LarryD. Bowers and Edward 1. Wong
562 A Radiolmmunoassay for 1,25-Dihydroxycholecaiciferol RogerBouillon. PieterDeMoor,Enrico C. Bagglolini, and Milan R. Uskokovic
568 AutomatedCreatine Klnase-MBEstimation by immuno-inhlbltlon: A ClinicalEvaluation ThomasJ. Delahurity and Craig C. Foreback 573 125I-Labeled Gonadoliberinof HighSpecificActivity and Inwaunoreactlvity: Method of lodination and Rapid Separation A. K. Sarda, N. A. Barnes,andR. N. G. Hair
579 DetermInation of Urinary AminoAcids by Liquid Chromatography with “Dabsyl Chloride” Jen-KunLinand Chiu-Hwa Wang
584 Serum High-Density Lipoprotein Cholesterol
638 Gas-Chromatographlc Determination of Disopyranilde and Its Mono-N-DealkylatedMetabolite in Serum with Use of a Nitrogen-SelectiveDetector
JanErik Bredesen 640 Ion Effects in Measurement of IonizedCalcium with a Calcium-Selective Electrode Richard A. Kaufman and Norbert W. Tietz 644 Quantitative Evaluation of Linearity
Robert W.Burnett 646 Lot-to-Lot Variation in Tlwee Thyroid-Related
Radiolmmunoassay Kits 649
652
MarkH.Zweigand E.Arthur Robertson Serum of Patients with ChronicRenal Failure Harvey A. SchwertnerandStevenB. Hawthorne StabIlityof Standard CurvesPrepared for EMIT
AlbumIn-Bound Fluorescence in
Homogeneous Enzyme Inimunoassay Kits Stored at RoomTemperature after Reconstitution Phillip A.Bach and JamesW.Larsen 654 Evaluationof StandardCurves In the Graded Hemagglutinatlon-InhlbltionMethod Applied to Quantitatlonof Human Immunoglobulins
ConcentrationsIn Hypo- and Hyperthyroldlsm Alfred G. Scottolini, NarhipuramV. Bhagavan, ThelmaH. Oshiro, and SuzanneV. Abe MosheWolk 588 AmnIoticFluid AlkalIne Phosphatase, Gamma658 Methodfor Determination of HydrogenPeroxide, Glutantyltransferase, and5’-NucleotldaseActivwith Its ApplicationIllustratedby Glucose ity from 13to40Weeks’ Gestation, andAlkaline Assay Phosphatase as an Index of Fetal LungMaturity ErnstGrafandJohnT.Penniston D. Brockletust andC. E.Wilde 592 Effects of Sample Aging on Total Cholesterol Values Determined by the Automated Ferrlc CASE REPORTS ChlorIde-SulfurIcAcidandLlebermann-Burchard Procedures 661 Case Report: Changes in Creatine Kinase IsoenPeter D. Wood,Paul S. Bachorik,JohnJ. Albers, zymes after Myocardlal Infarction In a PatIent CharlesC. Stewart, Carole Winn, and Kenneth with ProstaticCarcinoma Lippel Roy L Alexander,Jr. 598 Commerclally-Supplled Binders for PlasmaCobalamin(Vitamin B12),Analysls-”Pudf led”In- 664 ThyroxIneThyrotoxlcoels Mary1. Meistas,Leslie R. Sheeler, andNanjula S. trInsic Factor,“Ceblnamlde”-BlockedR-Protein Kumar Binder,andNon-purIfiedIntrinsicFactor-R-Proteln Binder-Compared with MicrobIologIcal Assay LETTERS NormanP. Kubasik, Margaret Ricotta,andHarrison E.Sine
601 Reference Values forCerebrosplnal FluidGlutamine Concentration inInfants Roger L. Boeckx,MarietIosefsohn,and Jocelyn N. Hicks 604 PurIficatIonof an Alkaline Phospliatase-Lipo-
profeln-XComplex A.N.N. Weijers,H.Kruijswijk, andJ. N.
Baak
609 PropertIes and Clinical Significance of an AlkalinePhospliatase-Upoprotein-X Complex R.N. N. Weijers 613 Fluorenietrlc and Coforimetric Enzymic Determinationof Triglycerides (Trlacylglycerols) In Serum HandaniWinartasaputra, Victorin N. Mallet, Shia
SPECIAL ISSUE
635 Interference of Imferon in Colorlmetrlc Assays for Iron W.Huisman
Various Factorsin DeterminingSpecificity LarryD. Bowers 555 KinetIc SerumCreatinlne Assays.II. A Critical
Reply Naomi0. Hansen,Charissa L. Oliphant, andWal’,d
C.Yasmineh
Assays
S. kuan, andGeorgeC.Guilbault 618 Improved Separati#{224}n of CreatineKinase Isoenzymes by Use of DEAE-SepftaroseCL-6B Richard J. L. Bonder, DouglasC. Shevchik,Met-
666 Polyethylene Glycol and SecondAntibody In Radloimmunoassay of Human Choriogonadotropin Iladassah Horn, Shlomith Berger,Amiram Bar-
Ilan,andZeevJonas of Liquid Control Materials for pH, Pco2, P02measurement
667 ComparIson
Linus B. F. Chang and
CallumG.Fraser
668 Interferenceof EDTAin Determinatonof c-AMP andc-GMPIn Plasmaby Radlolmmunoassay Pieter L. B. Bruynzeel,MaartjeL. Hamelink, and WillemvandenBogaard 669 Enzymlc AssayofCholine-ContaInIng Phospliolipids in Bile G. Annoni,N. L. Dioguardi,A. Tripodi, and N. Zuin
670 CrossReactivItyof DigitoxlnInRadiolmmunoassay and Enzyme-LinkedImmunoassayfor Dlgoxln in Plasma
J.N. ScherrmannandA. Bourdon Lead-Inhibited 5-Amlnolevullnate 671 ManualFerrozlne Kit Methodfor Iron and Total Iron-BindingCapacity Adapted and Evaluated Dehydratase Activityin Whole Blood by Heat,
YungHsu,andMargaretM. Kohler
of Diseases on ClinicalLaboratory Tests RichardB. Friedman,RenaeE. Effects
Anderson,StevenM. Entine, and ShellyB. Hirshberg
1938
CLiNICAL
625 RestoratIon of
Zinc Ion,and(or) Dlthlothreitol TadashiSakai, SusumuYanagihara, and Koichi Ushio
CIEMISTRY, Vol. 26, No. 13, 1980
for Usewith DiscreteMechanized Analyzers Stanley S.Levinson 672 Interconlparlson of Four Precipitation Methods
and Electrophoresis for High-DensityLipoprotein CholesterolDetermination Herbert H. Kohl, Wyvonne Johnson,and L. LeGroveAogers
673 MIsleadingResultsIn Casesof CoexistingAcetaminophenandSalicylateOverdose Jerald M. Aosenbaum, Henry H. Broer, and James Shields 674 Extraction of Underivatized Valproic Acid from Serum beforeGasChromatography David J. Freeman and Naresh Rawal 675 Serum Zinc as an Index to Myocardlal Infarc-
Non J. Cecil Smith,Jr. and James A. Halstead
675 Negligible Halothane Carryover
in the IL
813
BloodGasAnalyzer Lydia Chen Hood, WillaE.Noble,andElizabethK. Smith 676 Comparison of Radloimmunoassay Results in Serumand Plasma Joan E. Bauman Protoporphyrln/Herne Ratio by
677 Erythrocyte
Hematoftuorometry
724 High-Purity 4-Nitrophenot: Purification, Characterization,and Specifications for Useas a SpectrophotometricReference Material George N. Bowers, Jr., Robert B. McComb,RichardG. Christensen, and Robert Schaffer 730 CortisolDirectlyDetermined In Serumby Fiuorolmmunoaseay.wlth Magnetizable SolidPhase M. Pourfarzaneh, G. W. White, J. Landon, and D.
S. Smith
734 Plasma Methotrexate as
Determined by Liquid Chromatography, Enzyme-Inhibition Assay, and Radioimrnunoassay after High-Dose Infusion Scoff K. Howell, Yeu-Ming Wang, Ayohta Hosoya, and Wataru W. Sutow 738 Bilirubln Binding Capacity of Albumin Isolated from Cord-BloodSerum Is LessThan That from Serum of Adults Aviva Alayoff, Jaime Kapitulnik, Avraham Konijn, N. A. Kaufmann, and S. H. Blondheim 741 Variabilityin the AdsorptionProperties of Microtitre PlatesUsedas Solid SupportsIn Enzyme Immunoassay Larry J. Kricka, Timothy J. N. Carter, SusannahM. Burt, John H. Kennedy, Roger L. Holder, M. Isla Halliday, Margaret E. Telford, and G. Brian Wisdom
AngeloA. Lamola,JosefElsinger, and William E. Blumberg 678 SubstrateSpecificityof CholesterolOxidase 745 Enzyme-LinkedImmunoassayfor PlacentalLacLelandC. Clark,Jr. and ThomasA. Grooms togen in HumanSerum 679 Cholesterolin High-DensityLipoproteinsOuantiD. J. MacDonald, Kay M. Nicol, Alan Belfield, M. Iled by Agarose Gel Electrophoresls,Cellulose M.Shah, and D. S. Mack AcetateElectrophoresis, andMagnesIum-Sodi750 Measuring Thyroxine and Thyrotopin Simultaum Phosphotungstate Precipitation neouslyIn a Dried BloodSample on Filter Paper, E. Eugene Baillie, Peggy Welbom, Judy Hill, and to Screenfor Neonatal Hypothyroidism Brenda Sexton Shigenobu Nagataki, Kaichiro lshibashi, Ryuza680 Heel-StickBlood Samples Are Suitable for buro Ohsawa, Seizo Suwa. Nobuyuki Tsukamoto, Quantitation of Serum 1gMin Neonates Takeo Takahashi, Yashushi Obara, and Ying Yi Lily C. Yang, David Newman, Karen Greenfeld, Liao and Richard A. Polin 754 The Contribution of Assays for Lymphocyte Capping and Creatine Kinaseto Detection of the
681 BOOK REVIEWS
Peter Jatlow,J. Stanton King, and W. E. Cornatzer
683 THE
CLINICAL CHEMIST
J. S. King
MAY
Becker-Type Dystrophy Trait Barbara M. Goldsmith, Hanns-Dieter Gruemer, Rollin J. Hawley, Nathan A. Pickard, Harland L. Verrill, Walter E. Nance, Gregory Miller, and Ralph G. Crawford 760 lsoenzymes of Creatine Kinase in Extracts of Various Parts and Regions of the Human Central NervousSystem Royce R. L. Petronia, Anton H. J. Maas, Cees W. M. van Veelen, and Gerard E. J. Staal 763 SImplifiedApproachto Confidence LimitsIn Ra-
dioimmunoassay
691 REVIEW: The Expanding Roie of Microsomal En-
700
702
707
712
zyme Induction, and its Implicationsfor Clinical Chemistry David M. Goldberg FluorometricOuantitationof Glutamine in CerebrospinalFluid Isan Z. Liuand Hassan Khayam-Bashi LIquld-ChromotographicDetection of Thlazide Diureticsin Urine Philip A. Tisdall,ThomasP. Moyer, and John P. Anhalt Interferences Appearing in Fluorometrlcally Measured LIquid-ChromatographlcProfiles of Creatine Kinase Isoenzymesin Serum Timothy D. Schlabach, Joe A. Fulton, Peter B. Mockridge,and E. CliffordToren, Jr. Liquid-ChromatographicSeparationand On-Line
BIoluminescenceDetectionof CreatineKinase Isoenzymes William D. Bostick, Mark S. Denton, and Stanley
R. Dinsmore 718 Graphical Determination of Specific Activity, Binding Constants, and Antibody-Site Concentrations for Radioimmunoassays,with Application to Thymopoietin F. H. Verhoft, P. J. Lisi, C. 0. Fischer,J. W. Telpel, G. Goldstein, and M. B. Schiffman
Robert C. Baxter 766 Lamellar Body Phospholipid Content of Amniotic Fluid and US Ratio Compared in Assessing Fetal LungMaturity C. G. Duck-Chong. J. M. Gupta,G. N. B. Storey,
andC. A. S. Houghton
SCIENTIFIC NOTES
LETTERS 782 Overcoming Bilirubin transferase
Inhibition of
‘y-Glutamyl-
E. de Ia Morena
782 Interferenceby
Trlamterene with Assay
for
Metanephrine GanzaloMadiedoand Bernadine Smurawa
782 EvaluatIon
of Immunonephelometry of C-Reactive Protein In Serum GeorgesF#{233}rard, Cathy Goester, Th#{233}o Klumpp, and Pierre M#{233}tais 783 CatmagiteMethod for DetermInation of Serum Magnesium Modified Sidney A. Cohen and Irene E. Daza 783 More on a1-Globulln Zone-Splitting Phenomenon Francesco Aguzzi 784 Performance of the Oxygen-HemoglobinDissociation Analyzer (“Hem-O-Scan”), Compared with the IL 282 Co-O*lmeter H. L. Sehgal, L. R. Seghal, A. L. Rosen, A. DeWoskin, S. A. Gould, and C. S. Moss 784 Week-DayVariation In Clinical Chemical Analyses 6. Fex,H. Kristenson, and E. Trell 785 Diphenhydramlne Interferes with Determination of Phencyclidine by Gas-Liquid Chromatography Francis Avery Ragan, Jr., MonroeS. Samuels, Stephen A. Hite, and Ruby Ory 786 SerumAlkalinePhosphataseof Intestinal Origin: Detection by Acrylamide Gel Electrophoresis and L-p-BromotetramisoleInhibitionCompared Andrew W. SkillenandJuneHarrison 786 RapidLiquld-Chromatographic Measurementof 3-Methoxy-4-hydroxyphenylglycol and Other MonoamineMetabolitesIn Human Cerebrosplnal
Fluid Philip J. Langlais, William J. McEntee, and Edward 0. Bird 788 Interference of Cephalosporin Antibiotics with Analysis for Theophylline by LiquidChromatog-
raphy Dalmacio A. Agdeppa and Stewart 0. Lipton
789 StabIlityof Glucosein Urine Richard D. Worth, June Harrison, and Andrew W. Skillen 789 Probable Deficiencies in the DuPontacaMethod for CK-MB Jerome I. Marcus andLaurence M. Demers
789 AvoidingXanthlne InterferenceIn Urate Determination L. Stepatschuk 789 PersistentIncreaseof SerumLactateDehydrogenase Activity Related to Enzyme-IgG 790 Quarter-CenturyBibliographicReviewof Clinical
Chromatography on Polyacrytamide Gel Earl B. Weissman
772 ProblemsIn Radiolmmunoassay of HumanLutropinwith CommerciallyAvailable Reagents John E. Hammond, Julius C. Phillips, Carol B. Straight, and Mary G. Hammond 774 The Coefficient of Analysis, a New Figure of Meritfor Laboratory Performance Allan L. Louderback and Paul R. Szatkowski 776 Interferenceby the4-HydroxylatedMetabolite of Propranolol with Determination of Metaneptirines by the Pisano Method David Chou,MasanobuTsuru, JordanL. Holtzrnan, andJohn H. Eckfeldt 778 DecreasIng Sample Volume Used In the “Sequential Muitichannel Analyzer Computerized” (SMAC)
CORRECTIONS
(Lambda) Immunoglobulin Complex D.Acheampong-Mensah
770 Carclnoembryonic AntigenAssayedby Column
A. Stamper and D. M. Robertshaw
781
Chemistry Eugene W. Rice 792 Increased Creatine Kinase MB Values in Acute
AlcoholicIntoxication Paul Pottgen andB. R. Davis 792 Anomalous Resultswiththe CIS-SorinCorticotropin Kit A. Sharp, D. Handelsman, A. Ristuccia,R.Grigg, A. Conway, R. Baxter. and J. Turtle 793 Some Improvements in Operationof theTechnicon SMAC System Niall O’Leary and P. Finbarr Duggan 793 ThlazidesDo Not Affect Xanthlne Excretion in
HereditaryXanthinuria Ibrahim S. Salti and Kamal Hemady 794 Caveat in the Use of SerumThyroglobulinDeterminations for Monitoring Differentiated Thyroid Carcinoma Martin A. Schwartz
CLINICALCHEMISTRY,Vol. 26, No. 13, 1980
1939
794 Immunochemical and ElectrophoreticMethods for Determination of Lactate Dehydrogenase
lsoenzyme1 Compared Sunny S. Fike
795 A PrecautionInthe Useof TrichloroacetlcAcid (100g/L) asa ProteinPrecipitantfor BloodLactate Assay
J. W. Dolan, Sj. van der Wal, S. J. Bannister, and L. A. Snyder 881 A SemI-Automatic EnzymlcMethod for EstImating Urinary Oxalate Joan Yriberri and Solomon Posen 885 MechanIzed AmidolytlcTechnique for Determination of FactorX and Factor-XAntigen, and
DavidJ.Greenand RobertL.Perlman
798 BOOK
REVIEWS
Eugene K. Harris and RobertB.McComb
801
Edward S. Gill 1108 Responseof Creatine Kinase Isoenzymes to a New Creatine Kinase Immunoinhibitlon Test
Kit MichaelW. Bwgett,KennethErvin, Shing Kwan, and BarbaraPeasley
its ApplIcationto PatientsBeing Treatedwith
D.FraserDavidson 796 Protein-FreePlasmaPrepared by Ultraflftration beforeAssayof AscorbicAcid andUric Acid by LiquidChromatography
Desider A. Pragay, Susan F. Howard, and
Reply
OralAnticoagulants Eduard M. van Wijk, Laurens H. Kahl#{233}, and Jan W. ten Cate 891 DNA Nucleotldylexotransf erase of NormalPersons andLeukemic Patients Walid G. Yasmineh, Barbara M. Smith, and Clara
D.Bloomfield 896 Continuous-Flow Enzymatic Determination of TotalSerumCholesterol andMethodStandardizationwith CDC-Callbrated PooledSera M. A. MacAulay, C. L. Jacklyn, J. M. Mathers, and V. A. Storm
THE CLINICAL CHEMIST
Leo. 6. Morin
1109 Transient Hyperphosphatasemlaof Infancy: Four New Cases, anda Suggested Etiology SidneyB. Rosalki and Ying Foo Kinase lsoenzymeSB Concentration
1110 Creatlne
In Serum as a Marker of Myocardlal Infarction Andr#{233} C. Van Steirteghem, MarkH. Zweig, Roland M. Bernard,Gustaaf A. Putzeys, and Claude J. Bieva 1110 Effects of Heat and pH on Radlolmmunoassay and Activity of Prostatic Acid Phosphatase Jack Geller and Jerry Albert
J.StantonKing
SCIENTIFIC NOTES
JUNE
903 Applicability
805 REVIEW: Measurement of Tricyclic Antidepressants.Part Il. Applications of Methodology BruceA. Scoggins, Kay P. Maguire,Trevor A. Norman,andGrahamD. Burrows 816 A Coupled-EnzymeEquilibrium Method for MeasuringUreaIn Serum: Optimization and Evaluation of the AACC Study Groupon Urea Candidate ReterenceMethod Eric J. Sampson, Marie A. Baird, Carl A. Burtis, Emmalen M. Smith, David L. Witte, and David D.
Bayse
827 Spectrophotometrlc Determination of Urinary Oxalatewith Oxalate Oxidase Prepared from Moss
of Various Quality-Control Sera to Assay of High-DensityLipoprotein Cholesterol David C. Bullock, Timothy J. N. Caner, and Susan V. Hughes 907 Liquid-Chromatographic Assay of Urinary 5Hydroxy-3-lndoleacetlc Acid, with Electro-
Serum-Initiated
Viliam Lustig and Lyle W. Redman
835 LIquld-Chromatographlc Method for SimultaneousDetermination of Phenytoin and 5-(4Hy&oxyphenyl)-5-phenylhydantoln In Plasma and Urine Ronald J. Sawchuk and Linda L. Cartier
840 Hypophosphatasla (AdultForm):Ouantltatlon of SerumAlkalinePhosphatase IsoenzymeActivity in a LargeKindred Jos#{233} LuisMillhn, MichaelP. Whyte,LouisV. Avioli, and William H. Fishman 846 Recent Advances in Measurement of Amylase Activity-A Comparative Study Richard A. Kaufman andNorbert W. Tietz 854 Total Serum Cholesterolby Isotope Dilution! Mass Spectrometry: A CandidateDefinitive
Mod Alex Cohen, Harry S. Hertz,John Mandel,Robert C. Paule,RobertSchaffer, Lorna 1. Sniegoski, Tung Sun, Michael J. Welch, and Edward White
912 CORRECTIONS
JOINT MEETING, AACC and
CSCC 913 ScIentific Program 939 SymposIa Lecturers and Abstracts 960 Abstractsof Meeting Papers
1075 Workshops, Roundtables, and ExhibitorList 1087 New ProductsReview
LETTERS 1103 SeparatingLlpoproteinsIn 50 ILL of Serum: Heparln-MnCI2 andDextran Sulfate-CaCl2 Procedures Compared Arthur W. Kruski and DebraN. Weaver 1103 TechnIque for Decreasing theVolume of Liquid Radioactive Organic Waste JanetFletcher andPatrickConray 1104 PitfallsIn the Use of Serum IronConcentration in Diagnosisof Anemia Dennis Shapcott, Christian Fisch, and Yves
Poulin 1105 Urinary Oxalate Indirectly Determined by Continuous-Flow Analysisfor Calcium
V
A. H. Barry Sample, Marilyn B. Farber, and
861 CreatlneKlnase Radlolmmunoassay and IsoenzymeElectrophoresls Comparedin the Diagno-
Melvin A. Glick 1105 LInear Regression Analysis by Deming’s
sis of Acute Myocardlal Infarction HenryA. Homburger and Greg L. Jacob 867 SerumGastrins In Zollinger-EIlIson Syndrome: identificationof Localized Disease Jerome A. Johnson,Peter J. Fabri, and JohnA. Lott 871 On-LineLiquid-Chromatographlc Analysis for Drugs in Serum with the Technicon “FASTLC” System: PerformanceDatafor Theephylline andfor Four Commonly Used Antlconvulsants and Their Metabolites
Method D. S. Smith,N. Pourfarzaneh, andA. S.Kamel 1106 Factitious HyperuricemlaCausedby AboveNormal GlobulIns EricKaplan and Howard I. Finkle
1940
J. Stanton King
JULY 1119
AspartateAmino-
hydrogenase with Oxamate
THE CLINICAL CHEMIST
Zakariya K. Shihabi and JudyScaro 910 Liquld-ChromatographlcSeparation of Urinary 5-Hydroxy-3-IndoleacetlcAcid, with Measurement at 254nm Peter S. Draganac,Steven J. Steindel, and William C. Trawick
Meeuse transferaseAssay by inhibition of Lactate De-
1115
chemicalDetection
M. F. Laker, A. F. Ilofmann, and B. J. D.
831 Improved
1112 BOOK REVIEWS TheodorePeters, Jr., and Merle A. Evenson
1105 Temperature Control in Assay of Glycosylated Hemoglobins Andrea Mosca,Angelo Carenini, Michele Samaja,and VincenzoSaibene 1107ClinicalLaboratory Accidents,andSomeRecommended Remedies
CLINICALCHEMISTRY,Vol. 26, No. 13, 1980
Creatinine: A Review
SheshadriNarayananand HaroldD. Appleton 1127 IdentIfIcatIon of Fluorescent Pseudomonas Species Dennis C. Shelly, J. N. Quarles,and lsiah N. Warner 1133 Use of Soluble and SlaphylococcusaureusImmobilized Second Antibody Compared in a Radlolmmunoauay for HumanAlpha-Fetoprotein FrankE.Ruth, Jr., andGeorgeJ. Knight 1137 Effect of Cations on the Human CreatineKInase Isoenzymes Daniel A. Nealon and Arttinj- A. Henderson 1140 Radlolmmunoassayof Thyrotropin andThyroxho In Dried Blood by Use of Staphylococcus aweus Containing“Protein A” MarvinL. Mitchell, John J. McKenna, andJames
Mcfver 1143 EstrogenRadlolnvnunoassaySuitablefor the MonitorIngof OvulationInduction C. Dennis Ashby, HalC. Danzer,andRonaldS. Swerdloff 1147 SImultaneousMeasurementof Lecithin,Sphln-
gomyelk Phospfiafldylglycerol,P$iospliafldyhnositol, Phospiuatldylethanolamin., and Phosphatidylserlnein AmnioticFluid Pennell C. Painter
1152 Muscle Protein Analysis. N. Two-Dimensional Electropiioresls of Normal and Diseased Human Skeletal Muscle
C.S.Ciometti,N. Barany, N.J. Danon,andN.C. Anderson
1156An lnterlaboratory Evaluation of the IFCC Method for Aspartat. Atntnotransfsrasewith Useof PurifiedEnzymeMaterials Eric J. Sampson,Virginia S. Whither,Carl A. Bwlis, Sarah S. NcKnealIy,DouglasN. Fast, and DavidD. Bayse 1165 Effect of SerumpIt on StorageStability and Reaction LagPhaseof Human CreathoKin... lsoenzym.s
DanielA. Nealon,SabinaN.Pettit,andArthur R. Henderson 1170 Gas-Chromatographlc Determinationof Urinary Oxalate Ki V. Park and John egory
1173 Useof
the Conlugated Poly.n.
Fatty Acid,
Parinaric Acid, hi AssayingFatty Acids in Serum or Plasma CharlesB. Berde,JohnA. Kerner,andJohnD.
1178 Turbldimetryof inorganicSulfate, Ester Sulfate, andTotal Sulfurin UrIne Per Lundquist, Johannes M#{233}ilensson, 80 Sdrbo, and Stan Ohman 1182 Assay for Erytivocyte Uroporpliyrlnog.n I Synthase Activity, wIth Porplioblllnogen as Sub-
strate Roddey E. Ford, Ching-NanOu,andRalphD. El-
letson
1186 Radlolmmunoassay of
Free Thyroxine in Serum: Comparison withClinicalFindingsand Results
of
Conventional
Thyroid-FunctIon
Tests Monika F. Bayer andI. RossMcDou9all
1228 ResponsetoLetter Kit
on CIS-SormnCorticotropln
SamGonzales
1228SerumCollected with Uthium Unsuitable for Electrolyte and
lodoacetate Is Lactate Dehy-
drogenaseMeasurements E. ArthurRobinson, Ruth A. Chesier, and Ronald J. Elm 1229 Drug interferences hi Reactionsfor Detecting Hydrogen Peroxide by Meansby Peroxidase
Francesco ZoppiandDomenico Fenili 1230ClinicalApplication of Agarose-GelElectrophoresisIn IdentIfyingAbnormal H.mogloblns Stephen E. Vernon 1231Radlolmmunoassay of Free Cortlsol with Antiserum-coated Tubesand 125t4.th Cortlsol MariaCi’aziaDel Chicca, Aldo Clerico,Marco Ferdeghini, GuilianoMariani, Antonio Bol&mno, andCarlo Cipolloni 1232 improvedlon-ChromatographIc Methodfor DeterminingMg2’ and Ca2 InSerum Selwyn J. Rehfeld,Hans F. Loken,FrancisR. Nordmeyer,andJohnD. Lamb 1233 An Improved Automated Method forSerum
1193 2-MIcroglobuIhi Clearance as Measured by Radlolmmunoassay JannieWoo, MichaelFloyd,MaryAnn Langley, andDonaldC. Cannon 1198 Fluorometrlc Micromethod for Determinationof ArginaseActivity InDried Blood Spotson Filter Paper Adam P.Orfanos, EdwinW. Naylor,andRobert Cuthrie 1201QuantItative Analysis for Tricycllc Antidepressant Drugs in Plasmaor Serumby Gas Chromatography-Chemicai.lonlzatlon Mass Spectrometry Dennis N. Chinn, ThomasA. Jennison, Dennis J. Crouch, Michael A. Peat, and C. William
E. B. N. do Jong,H. M. J. Coldschmklt,A. C. C. N. van Alphen and J. A. Loog 1235 Sucrose Gradient Centrlfugatlon SedimentatIon Standards: A SinlpIifledTechnique Charles J. G-ossman, Patricia L. Vebel, LeonJ. Sholiton, Nathan
Evans,S. B. Malenkos,andJ. A. Coiweli 1209 improvements In Hemoglobin A, Determination
byRapidCation-Exchange Chromatography
J. StantonKing
of He-
moglobin, Percent Carboxyhemogloblnand Methemoglobln, and Concentrations of Total HemoglobinandOxygenIn Bloodof Man,Dog, aid Baboon R. C. Dennis and C. A. Valeri 1309 CommercIally AvailableBlood-Gas Quality Controls Compared withTonometered Blood Elizabeth Tong Leafy,y Graham,and Margaret A. Kenny 1317 Two-DImensionalGel Electrophoreslsof Carebrospinal FluidProteIns
DavidGoldman.Carl A. Merril, and MichaelH. Ebert 1323 Human ConjugatedBlIIrubIn-Isolatlon, Biosynthesis,and Direct MolecularCharacterlza-
than
Tal-Wing Wu, Nicholas Zurnbulyadis,Stanley Gross,andRolandS. Cohike 1336 Nitrogen Estimation in BiologicalSamplesby Use of Chemilumlnescence MichaelW. N. Ward. Christopher W. I. Owens, andMichaelJ. Rennie
AUGUST SCIENTIFIC NOTES 1241 REVIEW:SerumEnzymesand lsoenzymesin the Diagnosis and Differential Diagnosis of MyocardlaiIschsmlaandNecrosIs JohnA. LoftandJohnN. Slang 1251 An Optimized Assay of Alanine Aminopeptldase
CASE CONFERENCE
Activityin Urine KlausJungandDietmarScholz 1255 KinetIc Studies of a Quantitative Single-Tube Enzyms-LJi*ed Immunosorbent Assay VictorC. W. Tsang,Britt C. Wilson,andShirley
Landt,andMortonK. Schwartz
1304 Measuring Percent Oxygen Saturation
1340 Ollgoclonalinnmunogiobulins in Cerebrosplnal Fluid in Multiple Sclerosis Albert A. Keshgegian, JeanCoblentz,andRobert P. Lisak
Philip C. Randand Christine Nelson
1213 Colorectal Carcinoma and Carcinoembryonlc Antigen (CEA) Jack H. Ladenson,Jay N. McDonald, Michael
nlsolone-21-Hemlsuccinate by Extraction and TheIr Concurrent Determination by Radloimmunoassay KazuytAi Yanagibashi,Akira Mizuchi, Hideki Yotsumoto,and YninitakaMiyachi
CASE CONFERENCE 1237 THE CLINICAL CHEMIST
Thatcher 1205 lmmunonephelometrlc Assay of Human Apollpoprotein Al Maria F. L. Lopes-Virella,Gabriel Virella, C.
1298 Enzymlc Determinationof 3a-,7a-, and 12aHydroxyl Groups of FecalBileSalts Nichael J.CrowellandianA. Macdonald 1301 Separation of Serum Prednlsolonsand Pred-
of the Uroporplnyrmnogen I Synthasi Assay,anda ProposedModifiedMethod BernardA. ChamberlainandJosephE. Buttery 134$ Uquld-ChromatograplilcMeasurementof Uri-
1346 ReappraIsal
nary Monosaccharides MariusD’Amboise, Toshihiko Kanai,and Denis
Noel
1351ImprovedLlquid-ClwomatogaphlcDetermination of Caffein, in Plasma
James Blanchard,Judy D. Mohammadi,and KennethA. Conrad 1354 Effectolin VitroH.molyslson Assay of Plasma 1261 Catechoiamlnes andDOPA HI#{216}i-Density Loprotelns in Diabetes RichardT. Smith J. Dabor,N. Spain, and N. Kalant 1357 SImplified Method for Detecting Anti-insulIn 1266 Further Experience wIth Computer-Assisted AntIbodies and Insulin-Anti-insulIn Immune Diagnosisof Diseasesof the UverandBillary Complexes
E.Maddison Composition of Serum Very-Low-Densityand
Tree
SCIENTIFIC NOTES 1221TwoNew Non-Barbiturate Buffers forElectroplioresis of Serum Proteinson CelluloseAcetateMembranes JeffreyAmblerand Martin Rodgers 1224Two-SItehamunofluorometric Assay for Pregnancy-SpecIfic$1-Glycoproteln(SP1) Anne Sykes and T. Chard
LETTERS 1227 Commenton Linear Models Article N.J. A. Healy (Reply) Stanley N. Deming and Stephen L. Morgan
Gabriel Virella, DeboraRussell,MactaLaimins, GrahamEllis, Eric Worthy, andDavidN. GoldandJohnColwell berg 1272Fluorometry of Seleniumin Urine AzizGeahchanandPaulChambon LETTERS 1275 Determination of High-DensIty Lipoproteln Phoephoilpldsin Serum 1360Evaluationof BathoplienanthroiineMethodfor YoshihisaYamaguchi Serum Iron Assay Nada Majkic-Singh,Milka Koprivica. Slavika 1278 Kinetic Ethylene Glycol Assay with Use of Spasic, MarinaStoianov,andIvanBerke Yeast AlcoholDeIiy*ogsnase JohnH.Eckfeldtand A. ToddLight 1360 Creatlne KlnaseIsoenzymesIn TestIcular Tis1281MagnetIzable Solid-Phase Fluorolmmunoassay sue of NormalSubjectsandhi a Case of Lymof Plienytolnin Disposable Test Tubes phObiastIcLymphosarcoma R. S. Kamel, J. t.andon,andD. S. Smith GordonN. Hoag, RajpalSingh, Christopher A. 1285 -Upoproteln Ouantitatlon in CordBloodSpotFranks,andW. EarleDeCoteau 1361 TyphoId Fever Toxemia with Associated Deted on Filter Paper A Screening Test G. D. Vladutiu, C. J. Glueck.N. T. Schultz,S. structIonof SkeletalMuscle Mchleely,andR.Gutivie JohnA. Loft, Carl E. Speicher,and LeonaW. 1291 A New Kinetic Determinationof Serum5’-NuAyers cleotldase Activity, with Modificationsfor a 1362Determinationof a2-Macroglobutinin Amniotic CentrifugalAnalyzer Fluidin Diagnosisof NeuralTubeDefect JosephF. Dooleyand Linda Racich C.J. vanKampandJ. Calliauw
CLINICALCHEMISTRY,Vol. 26, No. 13, 1980
1941
1363InterferingImpurities In CommercialHigh-Pu-
I.
rity MethyleneChloridebeforeandafterEvaporation
AsaadN. Masoud,GeorgeA. Dubes,and Diane
1380 New Perspectives
(Reply) Kenneth R. Watson and Richard T. O’Kell 1365 Performance of Blood-Gas Confrols Quantra Arthur L. Schneider 1366 Some Comments on Antlconvulsant-DrugMon-
1392 Computer-Assisted
Commercially AvailableControl MaterIals forp Pco2and p
1366 Properties of
GeraldL. Vedaaand CarlChristianHolbek 1367Gas-ClvomatographlcMeasurement of Haioperidolin Plasma Michael Franklin 1368 indoxyl Sulfate interferes with Dip-and-Read UrinaryBilkubinEstimate A.C. Skjold,J. F. Freitag, L. R. Stover, and H. K. Berry
1369 Determination of High-DensityLipoprotein Cholesterolby Heparin-MnCI2Precipitation
sional Polyacrylamide Gel Eiectrophoresls Analyses Lewis E. Lipkinand Peter F. Lemkin 1413 Use of Magnetic CircularDichrolsm Spectroscopy for BiologicMonitoringof Occupational
Exposuresto Toxlcants E. R. Zygowicz,B. R. Hollebone,andH. M.Per-
Quarles 1425 Use of Electrochemlcal
Sensorsfor On-Line
Monitoringof ionizedCalcium, Potassium,and Glucose
InWholeBloodof LivingDogs
1942
CLINICAL CHEMISTRY,
1509 More on Quantitative EvaluatIonof Linearity Robert F. Martin says Jay L. BockandRichard Pierce
1511SomeComments on Linear Regression Analysisby the Methodof W. E. Doming N. A. Hathaway
II.
1512 ActIvation
of Aminotransferases by Pyridoxal 5-Phosphate:Importanceof Experimental De-
AnalytIcal Systems
tails
ViliamLustig (Reply)
J. M.C. HatkenscheidandC. C. M. Djit
1514 Glutamyitransferase Activity during Pregnancy Concepci#{243}n do Ia Piedraand EmiliaJerez Mass-Spectrometrlc Analysis 1514 Evaluation of Serum Monoamine Oxidase in R. Abbott,M. Anbar,H. Fadem,J. McReynolds, Patients with Connective TissueDiseases W.Rieth, N. Scanlon, L. Verkh, and B. Wolff Pasquale Oriente,AntonioRiccio,RaffalleScar1450 Continuous-Flow ZoneElectrophoresis:Experipa, LoredanaPostiglione, Angelo Span#{244}, and mental Demonstration and Discussion of PoAngelo Pucino
tential Clinical and ResearchApplications Egil Fosslien, DonnaN. Anderson, and Alan Nastir 1454 Automated MultipleFlow-Injection Analysis In Clinical Chemistry:Determination ofTotalProtein with Bluret Reagent
W. Aenoe 1459 Comparisonof TurbidimetricandLight-Scattering Measurementsof Immunoglobullns by Use of a Centrifugal Analyzerwith Absorbanceand Fluorescence/Light-Scatterlng Optics L. P.Hills and 1.0. Tiffany
Ill, High-Performance Liquid Chromatography (HPLC) 1467 CombInedLiquidChromatograph/Mass
Charles D. Scott,Carl A. Burtis,GerhardErtingshausen,andRobertS. Melville
LETTERS
Assay Error MarieKenny,A. F. Lambe, D. A. O’Kelly,andA.
1435 New Optical Techniquefor MeasuringErythrocyteDeformabillty with the Ektacytometer Warrenoner, Narla Mohandas,and Marcel Bessis 1443 DiagnosIsofViralInfections by Mufticomponent
J. Stanton King
1379 INTRODUCTION
Moser,DavidA. Wenger,andEdwinH. Kolodny by “HIgh-Performance” LiquidChromatography with Detectionat Multiple Wavelengths TimothyD. Schlabachand Seth A. Abbott
1504 Serum Protein Profiles
Darragh
1377 THE CLINICAL CHEMIST
Twelfth AnnualSymposiumon AdvancedAnalyticalConcepts for the Clinical Laboratory
M. DavidUllman, Reed E. Pyeritz, Hugh W.
1511 PhotosensItivity of Tryptophanasa Source of
Crump, Michael R. Wills, JohnSavory,andBrian
SEPTEMBER
doses
Cells Clinton D. Lothrop, Jr., and Mayo Uziel
CharlotteE. Shideler,Kent K. Stewart,John VernaL. Rausch, Warren N. Wise, Merle A. Evenson,Carry A. Rechnitz,Andr#{233} P. Do Leonhoer,Josef A. Jonckheere,Mario Werner,and RobertJ. Moflrbacher
Kabra,BrianStafford, andLaurenceMacton 1499 ApplIcation of “HIgh-Performance” Liquid ChromatographytotheStudy of Splningolipl-
1510 Trlmethoprim Interference In Methotrexate As-
Eric J. Fogt,ArthurR. Eddy,fr., Anton H. Clemens, John Fox,andHunterHeath,Ill 1430 Rapid Preparationof Nucleotld.s from AcidSolublePoolsby Chromatographyon Silicaas ExemplIfied with Acid Extracts of Cultured
Clinical Processor Can Be Updated Phillip R. Bach and JamesW. Larsen
1374 BOOK REVIEWS
William P. King and Peter T. Kissinger
1492 DeterminatIon of Organic Acids in Biological FluidsbyIonChromatography: Plasma Lactate and Pyruvate and Urinary VanllIylmandellc Acid WilliamRich,Edward Johnson, LouisLois,Pokar
kins
1419 Multiparameter Approach to the “Fingerprinting” of Fluorescent Pseudomonads Dennis C. Shelly,lsiah M. Warner, and JohnM.
SimonJ. 1’. Mao and BruceA. Kottke 1369 Multiple VenousSamples:A CautionaryTale J. D. PryceandJ. Durnford 1370A Standard Curve Stored in the SyvaCP-5000 1370 Excessive “Performance Ranges” Specified for ControlMaterialby the Supplier DennisP.Bogdan (Reply) SandyKrishnamurthy 1372MIstakenNotionabout “Correcting for Drift” RobertG. Hoffman 1372Adaptationsof Enzyme humunoassay for Thyroxine W. VogtandS. Braun 1373 Simple Adaptation of the DuPont acato Allow High-DensityLipoprotein CholesterolDetermination Arthur Smith, Paul Brown, Harold Crockett,and K. OwenAsh
Analysisof Two-Dimen-
sional Electrophoreses of HumanLymphold Cells Eric P. Lester,PeterLeiin, LewisLipkin,‘and HerbertL. Cooper 1403 Data-Base Techniques for MultipleTwo-Dimen-
itoringbyLiquidChromatography BrianE. Stafford, PokarN. Kabra,andLaurence J. Macton
in Coagulation Testing
JawedFareed,HarryL. Messmore,andEdward W. Bermes
N. Krupski 1364 Techniquefor Separating$-Lipoproteln as a Distinct Band intheSerum-Protein Electroplnoretlc Pattern on a Cellulose Acetate Strip N. Tarantino, E. Segat-Besta,and E. Longhi 1365 More on the RelationbetweenMg2 and P in Serum RobertWhangandTjien0. Oei
Reagents: Applications with In Situ Generated Bromine
AnalytIcal Concepts
1515 SerumNickelin Myocardlal Infarction J. N. H.Howard
1515 Reference Intervals forFerritln:
1516 jBls(2-hydoxyethyl)amlnojtrls(hydroxymethyl)methane Is an Effective BufferforCreatine KInase Assays Daniel A. Nealon, Sabina M. Pettit, andArthur R.
Henderson
1517 Effectof Sample Protein Corcentration on Results of Analyses for Sodium and Potassiumin Serum ChinShyrandC. C. Young 1517RelativeMerits of Creatine Kinase-MB Assay by lmmunolnhlbltion andby Electrophoresls MarioWernerand RobertJ. Mohrbacher (Reply) John A. Loft
Spec-
trometerfor InvolatlleBiologicalSamples C. A. Blakley,J. C. Carmody,andM. L. Vestal 1474 MlcrocolumnLiquidChromatography: A Toolof PotentialSignificance in Biomedical Research
1519 BOOK REVIEWS Carl do Shante,TheodorePeters,Jr., andJ. W.
Milos Novotny Goldzieher 1480 Specific Detectionof PrimaryAminesin the Effluentof a Gas-Chromatographic Column by On-LineMeasurementof Fluorescence FrancisChow andArthurKarmen 1521THE CLINICAL 1484 LIquid Chromatography with Amperometric ReactionDetection InvolvingElectrogenerated J. S. King
Vol. 26, No. 13, 1980
Age Depen-
dence B. Seamonds,K. Anderson,andB. Whitaker
CHEMIST
mogloblnA1 by AgarGelElectrophoresls 1628 Evaluation of a Kit for EarlyDetectionof PregLionel Menard,N. E. Dempsey,L. A. Blankstein, nancy H. Aleyassine,N. Wacks, andJ. S. Soeldner Manik Ganguly, Mortimer Levitz, Morton K. Schwartz,andJeromeS. Nisselbauni 1629 Detectionof NeonatalRespIratoryDistresson the BasIsof FluorescencePolarization(Micro1523 ElectrOphoretic, Kinetic, and Immunoinhibition SCIENTIFIC NOTES viscosity) Measurementsof Amniotic Fluid: A Propertiesof ‘y-Glutamyttransferase from Vail1603 “Ultra-Clean” IsotopeDilutIon/Mass SpectroWordof Caution ens Tissues Compared metricAnalysesfor Leadin HumanBloodPtasLeslie N. Shaw, LorettePetersen-Archer,Jack D.O.E. Gebhardt ma indicate That Most ReportedValues Are ArW.London,andElizabeth Marsh 1629 Chemotherapy as a Causeof LowSerumCr.tlficiaity High 1528 MonitorIng Serum ValprolcAcid by GasChroatine KinaseActivity JoelEversonand ClairC.Patterson Albert D. Fraser matographywIthElectron-CaptureDetection 1607 Radlolmmunoassay of Progesterone InUnex1630 A Non-DestructiveVisualizationReagent for S. C. Chan tracted Serum Use in Thin-LayerChromatographyof Sugars 1531 KineticInimunoturbIdlmetrlc Measurementof StephenP. Naynes,Jo N. Corcoran,CresweIlJ. ThyroxineBindingGlobulin and Rlbonucleosldes Eastman,andFredaA. Day A. B. BenkoandL. D. Szabo KevinSpencerand ChristopherP.Price 1610 Quantitatlonof SerumGentamlcin Concentra1631 StabIlityof Proslatlc Acid Phosphatase in Nor1537 MonItoring 2-Ethyl-2-phenylmslonamide in tion by a Solid-Phase Immunottuorescence Serum by Gas-LIquidChromatography:ApplimalHumanSera Method L. JosephsonandP. Houio cation toRetrospective Study in EpilepsyPaYuh-GengTsay, Lynda Wilson, andErin Keefe 1631 Before-AssayLlquificatlon of PulmonaryMutients Dosed with Primldone 1613 Advantage of Florisli over Charcoal Separation DanHaidukewychandErnstA. Rodin cous Secretions with N-Acetyl-L-cystelne in a MechanizedTestosteroneRadloimmunoPhilip K. U, JosephineT. Lee, and Linda N. 1540 EnzymlcDeterminationof Free Fatty Acids In assay Baker Serum S. Mitchell Harman, Panayiotis D. Tsitouras, H. Okabe,Y. Uji, K. Nagashima,andA. Noma 1632Useof TumorMarkersas a Supplementto CyMary AnnKowatch,and A. Avinoam Kowarski tology in Diagnosisof MalignantEffuslons 1544 RapId Radloimmunoassay for Prostate-Specific Acid Ptioephatase in HumanSerum G.Nilano.B. P.Krebs.H.Duplay,C. N. Lalanne, P’kko Wiko, Anja Kostama,Olli Janne, Esko andJ. P.Cassuto 1632 Prostate-SpecIfIc Acid Phosphatase Sajanti,andReijoVihko CASE REPORTS 1548 ComparabIlityof Resultsby Slmpilfled Methods RichardJ. Ablin 1617 TrlmethoprlmInterferes with SerumMethotrex(Reply) for Measuring SerumLipoproteinCholesterolin ate Assay by the Competitive Protein Binding a Pediatric Population JohnC. Goffiths Teciv SathanurR. Srinivasan,ThedaA. Foster,and 1633 PreparatIon of Protein-FreeSerumFiitratesby KennethHande,JonGober,andRonaldFletcher GeraldS. Berenson Centrlfugatlon through Supported Dialysis 1620 Detection of Alkaline Phosphatase/immuno1554 Gas Chromatographlc-Mass-Spectrometric Tubing globulinComplexes Identification of lsosaccharlno-l,4-IactoneIn DonaldA. GerberandStevenWeiss Joseph E. Buttery, Clyde R. Milner, Peter NenaHuman Serumand Urine 1634 VarIablein VivoBindingof Vaiproic Acid in Epdovic, and Peter A. Pannall Toshimitsu Niwa, Kenji Maeda, ToyokazuOhki, ilepsy Patients Being Medicated with Other Akira Saito, Jinsaku Sakakibara,andKaizo KoAnticonvulsants bayashi DanHaidukewychandErnstA. Rodin LETTERS 1562 Spectrophotometric Micro Method forMea1635 Creatine KlnaseBB lsoenzymeActivity in Pasurementof Dlalyzable Calcium byUseof Cretients with HematologicDisorders 1622SuItamethoxazoleInterfereswith Liquid-Chrosolpirthaleln ComplexoneandContinuous-Flow P. Joanne Cornbleet andN. Delell Evans matographic Analysis for Theophylline in Analysis Serum JohnTotfalettiandKathrynKirvan 1637 BOOK REVIEWS David B. Bowman, N. K. Aravind,RalphE.Kauff1566 Radiolmmunoassayof Human Thyroglobulin man,andJosephN. Miceli MortonK. Schwartz,A. L. Dryer,TheodoreA. W. with Use of “Thyroglobulln-Free”PlasmaPre1622Carein Useof Phenazine Methosulfate inthe Koerner, DavidSel’igson,JohnA. Loft,J. Stanton paredby Ultracentrlfugationas Diluent Laboratory King,J. W. GOIdZiehOr, andRussellP. Tracy Katsuji flekubo,Jerrilyn Jutton,and Arthur B. M. Worsfold Schneider 1622 Calibration of the Dextrometer/Dextrostlx Sys1643THE CLINICAL CHEMIST 1569 Antigen-AntIbody Reaction investigatedwith temfor theAssayof Whole BloodGlucose Useof a Chemically ModifiedElectrode ThomasC. Stewart J. StantonKing NaotoYamamoto,YoshikatsuNagasawa,Sada(Reply) nobu Shuto, Hiroshi Tsubomura,Masanobu DouglasA. Kavelman Sawai, and FiajimeOkumura 1623 Improved Llquid-ChromatographlcAssay of NOVEMBER 1573Concentration of Immunoreactive Trypsin and UrinaryCatecholamlnes Activity of Pancreatic Isoamylasein Serum aham P. Jackman Compared inPancreatic Diseases 1624 Blood/Plasma DistributionRatiosof PsychotroJanFatwenkrug and Erik Magid pic Drugs 1647 Enzyme Immunoassayof Hepatitis B Surface 1577 ImprovedAnalysisfor Urinary Polyamines by Kay P. Maguire, OrahamD. Burrows,TrevorR. AntigenwIthUseof the“Hepanostika” Test Kit Useof High-VoltageElectrophoreslson Paper Norman,andBruceA. Scoggins and a NewPhotometer, “Phofo-ElIsa 1” KeisukeFujita.ToshiharuNagatsu,KanShinpo, 1625 Immunoglobulln Fumuiro Tanaka, Keiko Nishi,KazutakaArisue, GIn CerebrospinalFluid KazufuroMaruta,RyogiTeradaira, andMasamiH.J. vander Helm CyozoHayashi,and KiyoshiMiyai chi Nakam4%a 1625 ManualFerrozineMethodfor Serum Iron and 1852 Single- and Coupled-Enzyme Nylon-Tube Re1533 RapId,Fully Automated Radiolnimunoassay of Total Iron-Binding CapacityAdapted toa Comactors for Plasma GlucoseDeterminationwith ProetaticAcid Phoephatasein Serum puter-DirectedAnalyzer,the GIllord 3500 Glucose Oxidase and Aldehyde DehydrogeS.Doss,N.L. Bowen,andK. D. Bagshawe NancyLaycock, MargaretHartlein,Zia Uddin, nase 1588 RapIdSodiumDodecylSulfate/Polyacrylamlde andJill Murray W.Hinsch,A. Antonijevit,andP.V. Sundaram GelElectropltoreslsof UrinaryProteins 1626 Acetoacetate and“Ketone” Interferencein Ki1656 ReactIvity of Amniotic Fluid Alpha-Fetoproteln ClaudeCachera,CharlotteMizon,Jean-Charles with ConcanavallnA in Relation to Gestational netic andContinuous-Flow Methodsfor CreatiFruchart,JacquesNizon,andAlbertTacquet nine Age: ClinIcal Implications 1591 Cofactor in Serum forHigh-Affinity Folate MelvinA. Glick, Wells R. Moorehead,Tjien 0. K. Toftager-I.arsen, E. Kjaersgaard,J. Chr.JaBindingin MBr Oei,andGaryA. Moore cobsen,andB. NOrgaard-Pedersen Jan Helm, Steen ingemannHansen,andJorgen (Reply) 1860 ZInc Concentrations in Serumas Relatedto Lynghye James W. North MyocardialinfarctIon J. LekakisandA. Kalofoutis 1593Modifiedaca MethodforDeterminationof Iron 1627A Rapid,SimpleAcetaminophen Determination Is Available 1862 ConcentratIonsof Magnesium,Caicluni, PotasChelated by Deferoxamineand Other ChelaJoseph N. Miceli and M. K. Aravind slum,andSodiuminHumanHeartMuscleafter tors (Reply) Acute MyocardialInfarctIon WilliamL. Steinmetz.MelvinR. Glick, and Tjien Jerald N. Rosenbaum,Henry H. Broer, and Michelle Speich,BernardBousquet,and GuyNi0. Oei JamesShields colas 1598 QuantItativeDeterminationof GlycosylatedHe-
OCTOBER
CLINICALCHEMISTRY,
Vol. 26, No. 13, 1980
1943
1866 C-TerminalParathyrin(ParathyroldHormone) Radlolmmunoassay in Serumwith Commerdaily AvailableReagents MarciaSimonandJoseCuan 1672DIagnosticUtlilty of C-Terminal Parathyrin Measurement as Compared with Measurementsof N-TerminalParathyrin andCalciumin Serum MarciaSimonandJoseCuan 1677 ResultswithSix “Kit” Radlolmmunoassays for PrimaryBile AcIds In HumanSerumIntercompared
Aldo Rode,Enrico Rode,Rita Aldini, Naixiz’,o Capefli,DavideFesti, ClaudiaSama, Giuseppe Mazzella,Antonio Maria Norselli, and Luigi Barbara 1883 Photometric Determinationof Glycosylatlon of Hemoglobinin Diabetes Mellitus Chan&ashekarV. Subramaniani, BhandaruRadhakrishnamurthy, andGeraldS. Berenson 1688 KinetIc Properties of ‘y-Glutamyitransferase from Human Liver Claude PetitClerc,Fran#{231}oise Schiele, Denyse
IvankaJerkunica,Judy Sophianopoulos,and DemetriosSgoutas 1738 Automated “Ganina-Flo” Radlolmmunoassay of Urinary Cyclic AMP
GaryBrookerandFeridMurad
CASE REPORTS 1741BenceJonesMyelomawith a Tetramer of
1765 Atypical Cathode-Migrating Creatlne Kinase lsoenzymeand HumanBreast Carcinoma:A SpecificMarker? TsanZ. Liu, Jen T. Shen, Yeu-TsuN. Lee,and StephenB. Shohet 1785 Separation of TheophyllinefromSulfamethoxazoleand Ampiciflln byLiquidChromatography Irving W. Frutkoff, GideonKidroni,and Jacob Menczel 1766 “Coefficient
of Analysis”: Justification Needed
RonPlait
Kappa-TypeGlobulin InSerum
N. 1. Gallango,R. Suinaga,andN. Ramirez 1745 Effect of Dlmethyl Sulfoxlde on SerumOsmolality DouglasN. RunckelandJ. RobertSwanson
BOOK REVIEWS 1767 ThomasA. Koch,HarveyKincaid,ChrisWalker,
SPECIAL REPORT 1748
Bagel, AhmedNahassen,and GerardS’,est
1694An hnmunochemlcalProcedureforDeterminationof Mltochondrlal AspartateAnilnotransferaseIn HumanSerum
Mary V. Tsourmas,Mario Werner,Irving Sunshine,andNormanP. Kubasik
IFCCProvisional RecommendatIon (1979)ona Scheme for a Two-Year Postgraduate Course
in CHnlcalChemistry C. J. Porterand D. H. Cumow
THE CLINICAL CHEMIST 1770J.StantonKing
LETTERS
RobertRej DECEMBER 1701 MagnetIzableSolid-PhaseFluorolmmunoassay 1754 Acetic Acid interferencewith Determinationof 5-Hydroxyindoleacetic Acid of Thyroxine by a Sequential Addition TechD. Slaunwhlte,A. Tuggey,andJ. Clements 1754 Some Aiternatives to the Proposed Selected 1775 A StudyoftheUse ofPolyethylene Glycol in A. D. Nargessi,J. Ackland,M. Hassan,G. C. Methodfor “AgaroseGel Electrophoresls” Forrest, D. S. Smith,andJ. Landon Estimating Cholesterol in High-DensityLipoKaarinaOjala andTheodorH. Weber 1704 SImultaneousLiquidChromatographyof 5-Fluprotein (Reply) orouracll, Urldlne, Hypoxanthlne, Xanthlne, P.N. N. Demacker,A. G. M.Higmans, H.E.VosJan-OlofJeppssonandCarl-BerthLaurell Uric Acid, Allopurinol, and Oxipurinol in Janssen,A. van’t Laar, and A. P. Jensen 1755 EnzymlcHigh-DensityUpoproteinCholesterol 1780 Measurementof High-DensityLlpoprotelnChoPlasma WallyE. Wungand Stephen B. Howell AnalyslsEffectsof Manganese lesterol in Serum:Comparisonof Six Isolation RaymondJ. Liedtke, Gory Kroon,and JohnD. MethodsCombinedwith EnzymlcCholesterol Batjer Analysis 1756 Creatine KinaselsoenzymeSB InHuman AmP. N. M.Demacker,H. E. Vos-.Janssen, A. G.N. SCIENTIFIC NOTES niotic Fluid Hijmans,A. van’t Lear, and A. P. Jansen ThomasJ. Delahuntyand Craig C. Foreback 1787 Evaluation of BaboonAntiserumto Carcinoem1756Spectropholometry of Anglofensln-Convertlng 1709 Fhiorometryof EthyleneGlycol inSerum bryonicAntigen JohnN. Meola,ThomasG.Rosano,andThomas Enzyme in Sarcoldosis DarrowE. Haagensen,Jr., CharlesE. Cox,WilA. Swift James1. TaylorandSharonFreeman liam G. Dilley,NicheleHansley,JanetMta’doch, 1710 Decrease in PlasmaGlucoseConcentration 1756 DetermInationof Serum Creatinine in the PresEdwardS. Newman,and SamuelA. Wells, Jr. ence ofHigh Concentrationsof Free Hemoduring Storage at -20 “C 1791 Assay for Trlmethoprimin Serum and Urine by OttavioGiampietro,RenzoNavalesi,Giuseppe Means of Ion-PairChromatography Buzzigoli,ClaudioBoni,andLucaBenzi H. L. Sehgal,L. A. Sehgal,A. L. Rosen,S. A. IanD. Watson,AlanShenkin.StuartJ.McIntosh, Gould,L. Dalton,andG. S. Moss 1713 Direct, Fixed-Time KineticAssaysfor 9-Hyand HowardN. Cohen 1757 UrInaryPhosphoethanolamlne: NormalValues droxybutyrateandAcetoacetate with a Centrif1796 ElectrothermalAtomic Absorption SpectrophougalAnalyzer ora Computer-BackedSpectroby Age tometry of CadnHumin Semen JohnRussellEastmanandDavidBlxler photometer Lawrence1. Wetzeland JohnU. Bell 1758 Xanthinurla: A New Case PhilipK. Li,JosephineT. Lee, Margaret H. 1800 VarIabIlItyin Unconlugated and TotalEstrlol in JohnH. KennedyandAdrianN. Semmence MacGillivray,Peter A. Schaefer,and John H. Serum during Normal ThIrd Trimester Preg1759SImplifiedSimultaneous Determinationof ValSiegel nancy 1718 Studyof an EnzymeImmunoassayKitfor CarprolcAcid andEthosuximideInSerumby GasL. L. PenneyandW.J. Kienke clnoembryonic Antigen LiquidChromatography 1804 Free AminoAcid Analysisof Untkned and 24-h A. KumpsandY. Mardens AichardMaiolini, Alain Bagel, CatherineChaUrineSamples Compared vance, Bernard Krebs, BernardHerbeth,and 1759 Dlmenhydrinate interferes with RadiolnununoMichaelY. Tsai, Jo G. Marshall,and MarkW. ReneMasseyeff assay of Theophylline 1723 Evaluation of a NewHomogeneous Enzyme InEdwardHahn 1809 ConcurrentMeasurements of Carcinoembryon1760 Modifled Acetonitrile Proteln-Precipltatlon hibitor Immunoassayof Serum Thyroxinewith ic AntIgen, Glucosephosphate isomerase, Method of SamplePreparation for Drug Assay Useof a BlchromaticAnalyzer “y-Giutamyitransferase, and Lactate DehydroPaulA. Finley,R. Jane Williams,and Don A. by LiquidChromatography genas. in Malignant, Normal Adult, and Fetal JamesC. MathiesandMargaretA. Austin Lichti Co Tissues 1726DIlutionas a Source of Error: Implicationsfor 1760 lgD-X Myeloma with Separate Heavy- and D. D. Munjal Preparation and CalibratIon of Laboratory Light-ChainM-Coniponents 1813A DIgitally LinearizedThermistorThermometer Jiiiani A. Vilpo and Kerttuirjala Standardsandfor Quality Control of RadlolmReferenced toIPTS-68 munoassays 1761 Nova 2 IonIzedCalcium Analyzer Compared R. L. Berger, T. Clem, C. Gibson, W. Siwek, and RoseE.GainesDas with the OrionSS-20 M.Sapoff LasseLarssonandStenOtiman 1729 Effect of Detergenton Kinetic JaNe-Method 1816 Macro Creatine Kinase:A Studyon CK-Llnked Assay of Creatine (Reply) lmmunoglobulin AndersBergmanandGOstaOtrman RobertL. Coleman Hojyo Yuu, Shuji Ishizawa, YasushiTakagi, KimihideGomi,OsamuSenu.andToruIshil 1732SpecificSerumPancreaticLipase Determina1763 Reversed-Polarity Immunoelectrophoresls 1821 Radlolmmunoassay of Creatlne Kinase B-Isotion, with Use of PurifiedCollpase TsietiSunandYork Y. Lien GeorgE. Ho$fmann and LudwigWeiss 1764 UltrafiltratIonas a MethodforSamplePreparaenzymes in Serum of Patientswith Azotemia, 1734 ImprovedUitraflitratlonMethodfor Determining tion, with SpecialReferenceto Urate Obstructive Ufopathy, or Carcinomaof the Prostate orBladder Unbound Corlisol in Plasma J. J. Pratt
1944 CLINICALCHEMISTRY.Vol. 26, No. 13, 1980
Henry A. Homburger, Sheldon A. Miller, and
OregL. Jacob 1825 DeterminatIonof 8-Methoxypsoralen InSuction-BlisterFluidandSerumbyUquidChromatography MarjaJ. Herfst,PeterN. Edelbroek,andFrederik A. de Wolff 1829 EnzymeImniunoassayof EstriolinPregnancy
Urine Narita K, Korhonen,Kari 0. Juntunen, and UlfH#{224}kan Stenman 1832 New Method for Llquid-Chromatographlc Measurement of Erythrocyte Protoporphyrinand Coproporphyrin MiriamSalmi and RaimoTenhunen 1836 Heparin-MagneslumChlorIde-AlbuminMethod for Enzymic Measurementof Cholesterol In -High-DensityLipoprotein Frank Tallet, Denis Raichvarg,and Jacques Canal
1839 Gas-ChromatographicMethod ofAnalysis for UrinaryOrganic Acids. I. Retention Indicesof 155MetabolIcally ImportantCompounds
Kay Tanaka,David G. Hine, AgnesWest-Dull, andTheodoreB. Lynn 1847 Gas-Chromatographic Method of Analysis for Urinary Organic Acids.II. Description ofthe Procedure, and Its Applicationto Diagnosisof Patientswith OrganicAcidurias
Kay Tanaka,AgnesWest-Dull,David G. Hine, TheodoreB. Lynn, andThomasLowe 1854 Double-AntibodyImmunoenzymeAssay for Human Prostatic AcidPhosphatase B. K. Choe,E. J. Pontes,M. K. Dong,andN. R. Rose 1860 Changes in TranscutaneousOxygen Tension duringCapillaryBlood-Gas Sampling Gary aham andMargaretA. Kenny 1864 Age-, Sex-,andRace-Related Differencesin Myoglobln Concentrationsin the Serum of Healthy Persons 1-WenChen, RuppertDavid, Harry A. Maxon, MatthewSperling,and Evan A. Stein
SCIENTIFIC NOTES 1869Analysisof FluorescenceQuenchingDataby the Methodof Levine DanielL. Parsons
1871 Measurement of Ultraflltrable
Calcium
in
Serum with Use of the “WorthingtonUftrafree AnticonvulsantDrugFilter” JohnH. EckfeldtandDonaldF. Koehler 1874 NovelReagentand Method for Direct Determination of Chloride In Serum with a Centrifugal Analyzer Wai Tak Law and GerhardErtingshausen
1877ClinicalChemicalValuesfor SomeCommon LaboratoryAnimals J. DonaldCaiseyand DuncanJ.King 1879 A 5TCo-Soap Assay forPlasmaTotal Non-Esterifled FattyAcidsCompared witha Gas-LiquidChr#{244}matographlc Method David C. Turnell, Christopher P. Price,and Michael M.France 1883 Measurement of UrinaryConstituents with the Astra-8 MultichannelAnalyzer ThomasA. Blumenfeld and BeverlyOriffith 1886 CountIng Efficiency of Some Commercially
CerebrosplnalFluid by Useof CelluloseAcetateElectrophoresls
Available Liquid Scintillators Compared
P.Oray,0. vanReenen,andG.N. Potgieter 1888 CreatineKinase lsoenzymePatterns in Neoplasmsof Peripheral Nerve J. Balaton,G.N.Hoag,C.R. Franks, C. A. Smith. andW. EarleDeCoteau 1890 Determinationof -Chorlogonadotropln in Human Plasma: Evaluation andComparisonof Five“Kit” Methods B. S. Knox,J.W. A. McKee,P.I. Hair,andJ. T. France 1895 Conductometric Thration of HydrochloricAcid in GastricJuice Anton P. van Zanten and Abrahamvan den Ende 1897 MuitlvarlateAnalysisof anEnzymicProfilefor the DifferentialDiagnosisof Viral Hepatitis GuyPlomteux 1899 lnterlaboratory Evaluationof Four RIAKitsfor Determinallonof PlasmaCortisol,with Special Reference to Accuracy: Influenceof Matrix In CalibrationStandards 0. Lantto, I. Bjorkhem,A. Blomstrand,and A. Kallner 1902 Evaluation of EnzymeImmunoassay,Radloas-
H. J. van der Helm,E. A. H. Hische,andH. K. van
Walbeek
1918 More on SubstrateSpecifIcity of Cholesterol Oxldase Charles J.W. Brooks and AndrewG. Smith
(Reply) Leland C. Clark, Jr., and Thomas Orooms 1919 Sodium 1,4’-Plperazln.dlethanes.ulfonate Monohydrate (“Pipes”) for Measurement ofpH of Blood and Other PhysIologIcal MedIa
RabindraM. Roy, James J. Gibbons,Jorge L. Padrort,KennanBuectiter,andStevenFaszholz 1920 AntibodyFractionationfor ImprovedSensitivity and SpecIfIcity of Antisera to Chorlogonadotropin 1. J. O’Brien.D. B. Seymour, and C. P. Morrow
1921 More on Direct Potentlometry-the Ion-SelectIve Electrodevs Flame Photometry
John0. CzabanandAlanD. Cormier (Reply) Robert L.Coleman,C.C. Young. andL. Sidoni 1924 AbnormalUrinary OllgosaccharldePattern in PatIentswIth GlycogenStorage Disease, Type
say, and Radloimmunoassay of SerumMetho-
III
trexate, as Compared with LiquidChromatography RobertG. Buice, William E. Evans, JamesKaras,
JohnMcLaren.
Won G. Ng,andThomas Roe 1925 Thyroxine-BIndIng Globulin Concentrations In thePlasmaof Severelylii PatIents
P. A. Pannall,W. A. Fuller,A. R. Peisach,Janet Marshall,SandraStuart,C. P. Reilly,andM. L.
CharlesA. Nicholas,ParamjeetSidhu,ArthurB. Straughn,Marvin C. Meyer, and WilliamA. Crom
1905 EvaluatIonof an Enzyme lmmunoassayfor0.termining Dlazepam and Nordlazepam in Serumand Urine Jack E. Wallace,StevenC. Harris,andEugene L.Shimek,Jr.
1907 CORRECTIONS
Weliby 1926 ModificatIon to the aca-A Manufacturer’s Comment ThatCan Be Generalized Robert L.Risacher 1926 Lyophllizatlonof Hemoglobin and the Stability of the Lyophllysates Pierre Labrude and ClaudeVigneron (Reply) DeanP. Bonderman 1927 The Unidentified Fluorescent Substance In
Serumof Patientswith ChronicRenalDisease Is Also Found in Hemollttrates, DialysisFluids, andUrine Harvey A. Schwertner, Susan T. Weintraub, and
CASE REPORTS 1908 Apparent MitochondrialCreatine KinaseInthe Serum of a Patient with Metastatic Cancerto the Liver JohnW. Heinz, Nickolas J. ODonnell,andJohn A. Lott 1912TotalCreatine Kinaseand lsoenzym. MB Activity in Serumand Skeletal Muscle of a Patient
with Dermatomyosltls SweiH. Tsung,TsauY. Huang.DanielHan,and Wei-PingLoh 1914 ApparentNormalLeukocyteAcid MaitaseAc-
Steven B. Hawthorne 1928 Race-Related DIfferences In Reference Intervalsfor Creatlne Kinase Michael Bright, Edward Wagman, Sharis Shastri, andMichaelNevins 1929 Improved Extraction of Valproic Acid from Serumbefore Chromatography James H. Goudie, Katheryn Reed. Gwendolen J. Ayers, andDavid Burnett
tivIty in Glycogen Storage Disease Type II (Pompe’s Disease)
JosephL. Potter,HaynesB. Robinson,Jr.,John D. Kramer,andIrwinA. Schafer
LETTERS 1916 AntlterritinLabeledwith the Bolton-HunterReXavierAlvarez-Hern#{227}ndez andAlvarLoria 1916 Radlolnununoassayof Cortisol in BloodCollected onFilter Paper Z. Kraiem,L. Kahana,V. Elias,S. Goersin,and M. Sheinfeld 1917 MonoclonalImmunoglobulinsDemonstrablein
1930
INVITED REVIEWERS-1980
1932 THE CLINICAL CHEMIST J. Stanton King
ANNUAL INDEX, VOLUME 26 1935 Contents 1946 Author Index 1960 SubJect index
CLINICALCHEMISTRY,Vol. 26, No.
13, 1980
1945