Table S1. Bacterial strains Strain Parental strain Relevant ... - PLOS

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FL905/pGS451. FL905. Φ(kan araC. araBp-lptC)1 ptac-. lptFR212GG_lptAB cat by transformation. This work. KG-280/pGS104. AM604/pKD46/pGS104 rpsL150.
Table S1. Bacterial strains Strain

Parental strain

AM604 AM604/pGS401 AM604/pKD46 AM604/pKD46/pGS104 FL905

MC4100 AM604 AM604 AM604/pKD46 AM604

FL905/pGS442

FL905

FL905/pGS443

FL905

FL905/pGS444

FL905

FL905/pGS445

FL905

FL905/pGS446

FL905

FL905/pGS447

Relevant characters Chromosomal Plasmid

Features/construction

Origin

bla bla ptac-lptCAB cat; bla

by transformation by transformation by transformation

[1] [2] [2] [2] [3]

ptac-lptFG cat

by transformation

This work

ptac-lptFR212CG cat

by transformation

This work

Φ(kan araC araBp-lptC)1

ptac-lptFR212SG cat

by transformation

This work

Φ(kan araC araBp-lptC)1 Φ(kan araC araBp-lptC)1

ptac-lptFG_lptAB cat ptaclptFR212CG_lptAB cat

by transformation

This work

by transformation

This work

FL905

Φ(kan araC araBp-lptC)1

by transformation ptaclptFR212SG_lptAB cat

This work

FL905/pGS450

FL905

ptac-lptFR212GG cat

by transformation

This work

FL905/pGS451

FL905

Φ(kan araC araBp-lptC)1 Φ(kan araC araBp-lptC)1

ptaclptFR212GG_lptAB cat

by transformation

This work

KG-280/pGS104

AM604/pKD46/pGS104

rpsL150 ΔlptCA::kan

ptac-lptCAB cat

by gene specific mutagenesis

[2]

rpsL150 rpsL150 rpsL150 rpsL150 Φ(kan araC araBp-lptC)1 Φ(kan araC araBp-lptC)1 Φ(kan araC araBp-lptC)1

KG-286.01/pGS104

KG-280/pGS104/pCP20

rpsL150 ΔlptCA rpsL150 ΔlptCA rpsL150 ΔlptCA rpsL150 ΔlptCA

ptac-lptCAB cat

KG-286.02/pGS308

KG-286.01/pGS104

KG-286.03/pGS315

KG-286.02/pGS308

KG-286.04/pGS308

KG-286.03/pGS315

KG-286.05/pMBM07

KG-286.04/pGS308

rpsL150 ΔlptCA

araBp-lptCA amp rpsL+ repA101ts

KG-286.06/pGS404

KG-286.05/pMBM07

rpsL150 ΔlptCA

ptac-lptC_lptA cat

KG-286.10/pGS321

KG-286.05/pMBM07

rpsL150 ΔlptCA

ptac-lptA cat

KG-286.07/pGS406

KG-286.05/pMBM07

rpsL150 ΔlptCA

ptac-lptCH cat

KG-286.13/pGS416

KG-286.05/pMBM07

rpsL150 ΔlptCA

ptac-lptAB cat

KG-286.14/pGS416

KG-286.05/pMBM07

rpsL150 ΔlptCA

ptac-lptAB cat

KG-286.21/pGS308

KG-286.06/pGS404

rpsL150 ΔlptCA

ptac-lptCA kan

KG-286.22/pGS305

KG-286.01/pGS104

rpsL150 ΔlptCA

ptac-lptCAB kan

ptac-lptCA kan ptac-lptCAB-tetAt cat rpsL+ ptac-lptCA kan

by FLP-mediated kan cassette excision by plasmid shuffling; selection for KanR, screening for CamS by plasmid shuffling; selection for CamR StrS, screening for KanS by plasmid shuffling; selection for KanR StrR, screening for CamS

[2]a

by plasmid shuffling; selection for AmpR, screening for StrS, 28 °C. Parental, sequenced by plasmid shuffling; selection for CamR StrR at 42 °C, screening for AmpS by plasmid shuffling; selection for CamR StrR at 42 °C, screening for AmpS lptA+ ΔlptC by plasmid shuffling; selection for CamR StrR at 42 °C, screening for AmpS lptH ΔlptA

[2]a

This work This work [2]a

[2]a This work [2]a

by plasmid shuffling; selection for CamR StrR at 42 °C, screening for AmpS lptA+ ΔlptC by plasmid shuffling; selection for CamR StrR at 42 °C, screening for AmpS lptA+ ΔlptC by plasmid shuffling; selection for KanR, screening for CamS lptC+

This work

by plasmid shuffling; selection for KanR, screening for CamS lptC+

This work

This work This work

KG-292.01/pGS321

KG-286.05/pMBM07

KG-292.02/pGS308

KG-292.01/pGS321

KG-292.03/pGS323

KG-292.01/pGS321

KG-293.01/pGS321

KG-286.05/pMBM07

KG-293.02/pGS308

KG-293.01/pGS321

KG-293.03/pGS323

KG-293.01/pGS321

KG-294.01/pGS416

KG-286.05/pMBM07

KG-294.02/pGS305

KG-294.01/pGS416

KG-294.03/pGS324

KG-294.01/pGS416

KG-295.01/pGS321

KG-286.05/pMBM07

KG-295.02/pGS308

KG-295.01/pGS321

KG-295.03/pGS323

KG-295.01/pGS321

rpsL150 ΔlptCA; lptFR212C rpsL150 ΔlptCA; lptFR212C rpsL150 ΔlptCA; lptFR212C rpsL150 ΔlptCA; lptFR212S rpsL150 ΔlptCA; lptFR212S rpsL150 ΔlptCA; lptFR212C rpsL150 ΔlptCA; lptFR212S rpsL150 ΔlptCA; lptFR212S rpsL150 ΔlptCA; lptFR212S rpsL150 ΔlptCA; lptFR212G rpsL150 ΔlptCA; lptFR212G rpsL150 ΔlptCA; lptFR212G

ptac-lptA cat

by plasmid shuffling; selection for CamR StrR at 42 °C, screening for AmpS lptA+ ΔlptC. Sequenced. by plasmid shuffling; selection for KanR, screening for CamS lptC+

This work

ptac-lptA kan

by plasmid shuffling; selection for KanR, screening for CamS lptC-

This work

ptac-lptA cat

by plasmid shuffling; selection for CamR StrR at 42 °C, screening for AmpS lptA+ ΔlptC. Sequenced. by plasmid shuffling; selection for KanR, screening for CamS lptC+

This work

ptac-lptA kan

by plasmid shuffling; selection for KanR, screening for CamS lptC-

This work

ptac-lptAB cat

by plasmid shuffling; selection for CamR StrR at 42 °C, screening for AmpS lptA+ ΔlptC. Sequenced. by plasmid shuffling; selection for KanR, screening for CamS lptC+

This work

ptac-lptAB kan

by plasmid shuffling; selection for KanR, screening for CamS lptC-

This work

ptac-lptA cat

by plasmid shuffling; selection for CamR StrR at 42 °C, screening for AmpS lptA+ ΔlptC by plasmid shuffling; selection for KanR, screening for CamS lptC+

This work

by plasmid shuffling; selection for KanR, screening for CamS lptC-

This work

ptac-lptCA kan

ptac-lptCA kan

ptac-lptCAB kan

ptac-lptCA kan ptac-lptA kan

This work

This work

This work

This work

KG-296.01/pGS416

KG-286.05/pMBM07

KG-296.02/pGS305

KG-296.01/pGS416

KG-296.03/pGS324

KG-296.01/pGS416

KG-297.01/pGS416

KG-286.05/pMBM07

KG-297.02/pGS305

KG-297.01/pGS416

KG-297.03/pGS324

KG-297.01/pGS416

KG-299.01/pGS321

KG-286.05/pMBM07

KG-300.01/pGS321

KG-286.05/pMBM07

KG-301.01/pGS321

KG-286.05/pMBM07

KG-302.01/pGS416

KG-286.05/pMBM07

KG-303.01/pGS416

KG-286.05/pMBM07

rpsL150 ΔlptCA; lptFR212G rpsL150 ΔlptCA; lptFR212G rpsL150 ΔlptCA; lptFR212G rpsL150 ΔlptCA; lptFR212C rpsL150 ΔlptCA; lptFR212C rpsL150 ΔlptCA; lptFR212C rpsL150 ΔlptCA; lptFR212G rpsL150 ΔlptCA; lptFR212G rpsL150 ΔlptCA; lptFR212S rpsL150 ΔlptCA; lptFR212S rpsL150 ΔlptCA; lptFR212C

ptac-lptAB cat

by plasmid shuffling; selection for CamR StrR at 42 °C, screening for AmpS lptA+ ΔlptC by plasmid shuffling; selection for KanR, screening for CamS lptC+

This work

ptac-lptAB kan

by plasmid shuffling; selection for KanR, screening for CamS lptC-

This work

ptac-lptAB cat

by plasmid shuffling; selection for CamR StrR at 42 °C, screening for AmpS lptA+ ΔlptC by plasmid shuffling; selection for KanR, screening for CamS lptC+

This work

ptac-lptAB kan

by plasmid shuffling; selection for KanR, screening for CamS lptC-

This work

ptac-lptA cat

by plasmid shuffling; selection for CamR StrR at 42 °C, screening for AmpS lptA+ ΔlptC by plasmid shuffling; selection for CamR StrR at 42 °C, screening for AmpS lptA+ ΔlptC by plasmid shuffling; selection for CamR StrR at 42 °C, screening for AmpS lptA+ ΔlptC by plasmid shuffling; selection for CamR StrR at 42 °C, screening for AmpS lptA+ ΔlptC by plasmid shuffling; selection for CamR StrR at 42 °C, screening for AmpS lptA+ ΔlptC

This work

ptac-lptCAB kan

ptac-lptCAB kan

ptac-lptA cat ptac-lptA cat ptac-lptAB cat ptac-lptAB cat

This work

This work

This work This work This work This work

NR1113

NR754

NR1113/pGS401 NR1113/pGS442 NR1113/pGS443 NR1113/pGS444 NR1113/pGS450

NR1113 NR1113 NR1113 NR1113 NR1113

a

(λattlom)::bla araBp-lptFG ΔlptFG araBp-lptFG araBp-lptFG araBp-lptFG araBp-lptFG araBp-lptFG

[4]

ptac-void ptac-lptFG cat ptac-lptFR212CG cat ptac-lptFR212SG cat ptac-lptFR212GG cat

by transformation by transformation by transformation by transformation by transformation

This work This work This work This work This work

KG-286/pGS104 parental strain and its derivatives described by [2] have been renumbered by adding decimal figures to the host collection name

just to distinguish individual KG-286 derivatives, as they may have acquired different mutations upon selection by plasmid shuffling.