Fisher's. Exact Test p-value. Figure S1B. WT1-433. 308 193. GFP. 0. 84. 2.15 x 10-31. WT1-15. 0. 93. 2.79 x 10-34. WT1-20. 154 54. 1.35 x 10-3. WT1-41. 314 34.
Table S1. Statistical analysis of qualitative live cell imaging data. Fisher’s exact pvalues for qualitative analysis. Mitotic BS-C-1 cells expressing GFP-tagged constructs were visually scored for the presence or absence of chromosome association. P-values comparing the frequency of cells exhibiting chromosome association were determined using a Fisher’s exact test as described in the Methods. Abbreviations: N1(ON CHR) = number of cells expressing construct 1 with positive chromosome association; N1(OFF CHR + CC) = number of cells expressing construct 1 that had exclusion from mitotic chromosome or were “cannot call”. N2(ON CHR) = number of cells expressing construct 2 with positive chromosome association; N2(OFF CHR + CC) = number of cells expressing construct 2 that had exclusion from mitotic chromosome or were “cannot call”. The “OFF CHR” and “Cannot Call” were combined in order to have a 2-variable analysis that compared the frequency of cells that exhibited chromosome association.
