Gene product/DR nucleotide sequence (5'-3') 23S rRNA methyltransferase (formerly known as open reading frame X conserved hypothetical protein)e AGAAGCGTATCACAAATGA β-lactamase protein BlaZ
Penicillin binding protein 2a
Signal transducer protein MecR1 Beta-lactamase regulatory protein BlaR1
Methicillin resistance regulatory protein MecI Beta-lactamase regulatory protein BlaI
ORF no. SH0213 S. haemolyticus strain JCSC1435 (AP006716.1) ORF ACJB01000028.1 S. epidermidis M23864:W1 contig00029
ORF_120
22852-24501
ORF_125 (arsCM10/0061)
27751-28143 (complement)
ORF_126 (arsBM10/0061)
28161-29450 (complement)
ORF_127 (asrRM10/0061)
29450-29764 (complement)
ORF no. SH0213 S. haemolyticus strain JCSC1435 (AP006716.1) ORF ACJB01000028.1 S. epidermidis M23864:W1 contig00029 (ACJB01000028.1) ORF no. SSP0088 S. saprophyticus strain ATCC15305 (AP008934.1) OFR no. SSP0089 S. saprophyticus strain ATCC15305 (AP008934.1) ORF STACA0001_1525 Staphylococcus capitis SK14 (ACFR01000014.1) ORF GG730274.1:513166-513858 S. aureus C160 (GG730274.1) ORF STAWA0001_0106 Staphylococcus warneri L37603 (ACPZ01000067.1) ORF GL545278.1:540491-541666 Staphylococcus caprae C87 (GL545278.1) arsC S. capitis SK14 (ACFR01000004.1) arsC S. haemolyticus JCSC1435 (AP006716.1) arsC MRSA252 (BX571856.1) arsB Staphylococcus xylosus plasmid pSX267 (M80565.1) arsB MRSA 252 (BX571856.1) asrR S. aureus IMCJ1379 plasmid pSA1379 (AB255366.1)
DR-2
29884-29902
n/a
ORF_128
29941-30855
ORF_121
24867-25916
ORF_122
25934-26602
ORF_123
26743-27423 (complement)
ORF_124
27544-27714 (complement)
BAE03522.1
75%
FAD-binding dehydrogenase
EES41411.1
75%
BAE17233.1
84%
ABC transporter permease protein LolE
BAE17234.1
89%
ABC transporter ATP-binding protein
EEE48440.1
93%
Flavin reductase like domain protein
EFC01991.1
93%
EEQ79095.1
41%
EFS16465.1
41%
EEE49308.1
92%
Arsenate reductase
BAE03410.1 CAG39708.1 AAA27588.1
92% 91% 93%
Arsenic efflux pump protein
CAG39707.1 BAE92849.1
93% 79%
Arsenic resistance operon repressor protein
n/a
n/a
DUF1837 superfamily protein
AGAGGCGTATCATAAGTAG
EFV36237.1 52% Hypothetical protein ORF HMPREF0432_00311 Gemella moribillum M424 (ACRX01000004.1) ORF _129 30855-31700 53% DNA helicase protein ORF Sez_1540 Streptococcus equi subsp. ACG62873.1 zooepidemicus MGCS1056 (ACG62873.1) a _130 31697-32962 ACG62873.1 39% DNA helicase protein XI in M10/0061 and ORF Sez_1540 Streptococcus equi and subsp. TheORF final three ORFs listed in the table (ORF_128, ORF_129 ORF_130) are located downstream of SCCmec zooepidemicus MGCS1056 (ACG62873.1) n/a ORFs is flanked n/a AGGAGCGTATCATAAATAG n/a However the region harboring these three are notDR-3 part of the 33024-33042 SCCmec element. by direct repeat (DR) sequences and is
considered to be a remnant of a SCC element and is therefore included in the table.
b
Nucleotide coordinates based on the nucleotide sequence of SCCmec XI of M10/0061 (GenBank accession number FR823292).
c
For all ORFs identified within M10/0061, apart form the mec complex genes, only the top hit identified using BLAST is shown.
d
Amino acid identities between two sequences were calculated from global sequence alignments. The sequences were aligned with the
needle global alignment program from the EMBOSS software package (45). Relative identities were calculated from the number of identical amino acids in the alignment with respect to the length of the new sequence from SCCmecXI. These overall amino acid identities were calculated for the best matching BLAST sequence and for some other staphylococcal sequences which represent different allotypes or paralogs of that gene. d
orfX has been annotated as a 23S rRNA methyltransferase (http://www.uniprot.org/uniprot/P0C1V0).