Table SIV: Summary of monoclonal antibody ...

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Table SIV: Summary of monoclonal antibody characteristics. Rearrangement. Original. Ig isotype*. Heavy Chain. Light Chain. Patient. Ab. Specificity.
Table SIV: Summary of monoclonal antibody characteristics Rearrangement Original Ig isotype* Patient

Ab

Specificity (ELISA/Array)

AT11

1.C04 1.B03 1.C07 1.A01 1.C06 1.B02 1.C01 1.C03 1.D03 1.B12 1.A04 1.D11 1.C12 1.A05 1.B04 1.B06 1.D06 1.D07 1.A02 1.A06 1.D01 1.A11 1.B01 1.B11 1.C09 1.D04 1.B05 1.B12 1.C02 2.A05 2.B02 2.B11 2.C04 2.C10 1.C04 1.C12 1.A07 1.B04 2.C05 2.D05 2.B05 1.C01 1.D06 2.A08 2.B06 2.C07 2.D01 2.E01 1.G01 1.D03 1.B02 2.B01 2.C02

CtxA/Holotoxin CtxB CtxB CtxB CtxB CtxB CtxB CtxB CtxB FlaA† LPS/OSP LPS/OSP PPK† Sialidase Sialidase Sialidase Sialidase TagA† Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined CtxA/Holotoxin CtxA/Holotoxin CtxA/Holotoxin CtxA/Holotoxin CtxA/Holotoxin CtxB CtxB CtxB CtxB CtxB CtxB CtxB CtxB CtxB Sialidase Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined CtxA/Holotoxin CtxA/Holotoxin CtxA/Holotoxin CtxB CtxB CtxB

AT13

CF21

Clonal expansion per patient 1 1 4 4

3 6 6

2 2 3 5 5

1 1 1 1 1 2 2 3 4 4

4 4

Heavy Chain

Light Chain

Heavy chain

Light chain

V

J

Mutations

V

J

Mutations

IgG1 IgG1 IgG1 IgG2 IgG1 IgG1 IgG1 IgG1 IgG1 IgM IgM IgM IgG1 IgA1 IgG1 IgG1 IgG1 IgG2 IgA1 IgA1 IgM IgA1 IgA1 IgG2 IgG2 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgA2 IgG1 IgG1 IgG1 IgG1 IgG1 IgG2 IgG1 IgG1 IgG1 IgG2 IgG1 IgG2 IgG1 IgG1 IgG1 IgA1 IgG1 IgG1 IgG1 IgG1 IgG1

Igκ Igκ Igκ Igλ Igλ Igκ Igλ Igκ Igκ Igλ Igλ Igλ Igλ Igλ Igκ Igκ Igλ Igλ Igκ Igκ Igλ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igλ Igλ Igλ Igλ Igλ Igκ Igλ Igλ Igλ Igκ Igλ Igλ Igκ Igλ Igλ Igκ Igκ Igκ Igκ Igκ Igκ Igλ

3-21 5-51 5-51 3-23 3-23 4-39 3-23 5-51 4-61 4-39 3-64 3-64 7-4 3-30 3-30 3-30 3-21 4-59 5-51 5-51 4-39 1-18 1-18 3-15 3-23 3-21 3-21 3-21 3-21 3-21 3-21 3-11 3-11 3-15 3-11 3-11 5-51 7-4 4-39 3-11 3-9 4-28 7-4 3-53 4-59 4-39 3-21 3-21 3-21 4-31 1-2 4-59 3-30

3 5 5 6 6 5 4 5 4 5 4 4 5 6 4 5 4 5 5 4 5 4 4 5 4 4 4 4 4 4 4 4 4 6 4 4 4 1 5 3 6 5 4 6 4 5 3 3 3 4 5 4 4

16 8 10 48 20 23 30 11 34 11 16 10 28 27 12 7 21 14 36 24 24 17 13 13 19 19 24 22 28 30 34 30 31 28 31 31 18 24 28 8 12 42 21 33 26 27 21 16 23 27 24 28 32

4-1 1-12 1-12 3-21 3-21 1D-33 1-40 1-16 1-39 1-40 2-14 2-18 2-8 3-25 1-16 1-39 4-60 1-40 4-1 4-1 1-40 1-39 1-39 3-20 1-6 3-20 3-20 3-20 3-20 3-20 3-20 1-40 1-40 1-40 1-40 1-40 3-20 1-40 2-23 1-44 3-11 1-51 7-43 1-39 3-21 2-8 1-39 4-1 4-1 2-30 1-39 1-39 7-46

1 4 4 3 2 4 2 4 2 2 1 1 2 2 5 4 2 2 5 5 2 5 5 1 4 5 2 2 2 2 2 3 3 1 3 3 2 3 1 2 2 3 3 1 3 3 4 1 1 1 5 3 2

13 9 13 16 19 13 8 24 22 5 15 6 19 14 7 4 17 9 31 25 10 12 12 9 15 10 19 21 17 22 23 16 20 27 18 13 16 23 15 5 9 28 25 25 13 17 19 9 17 10 29 19 19

CF29

CF30

1.A02 1.A03 1.A04 2.F01 1.B04 1.C01 1.D04 1.F01 1.D01 2.G01 1.C03 1.B01 1.F02 1.E05 1.A01 2.D01 1.E03 1.E05 1.A05 1.A04 1.C01 1.A06 1.B02 1.B05 1.C02 1.G01 1.G02 1.E06 1.A01 1.A02 1.C04 1.G04 1.G05 1.C06 1.B03 1.B06 1.C03 1.D01 1.F06 1.G03 1.A03 2.E02 1.E06 1.A05 1.D04 2.G02 2.E05 2.B05 2.A03 2.G05 1.A06 2.C01 1.E02 1.E03 1.A02 1.C01 1.C03 1.C05 2.A01 2.A02

LPS/OSP LPS/OSP LPS/OSP LPS/OSP LPS/OSP LPS/OSP LPS/OSP LPS/OSP LPS/OSP LPS/OSP LPS/OSP Undetermined Undetermined Undetermined Undetermined Undetermined CtxB CtxB CtxB CtxB CtxB LPS/OSP LPS/OSP LPS/OSP LPS/OSP LPS/OSP LPS/OSP LPS/OSP LPS/OSP LPS/OSP LPS/OSP LPS/OSP LPS/OSP Sialidase Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined CtxA/Holotoxin CtxB CtxB Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined Undetermined

1 1 1 1 2 2 2 2 3 3 1 1

3 3 3

1 1 1 1 1 1 2 2 2 2 2 2

3 3 1 1 2 4 4 5 5 6 6

IgA2 IgA2 IgA2 IgA2 IgA1 IgA2 IgA2 IgA1 IgA1 IgG1 IgG2 IgA2 IgA2 IgG2 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgA2 IgA2 IgA1 IgG2 IgA1 IgA1 IgG1 IgA1 IgG1 IgG1 IgA1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgG1 IgA1 IgA1 IgA1 IgM IgM IgM IgM IgA1 IgA1 IgG1 IgG1 IgG1 IgG1 IgG2 IgG2 IgG2 IgG1

Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igλ Igλ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igλ Igλ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igλ Igλ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igκ Igλ Igκ Igλ Igκ Igλ

3-23 3-23 3-23 3-23 3-73 3-73 3-73 3-73 3-15 3-15 3-53 3-23 3-23 6-1 1-69 3-21 3-7 3-7 3-7 3-23 1-18 3-7 3-7 3-7 3-7 3-7 3-7 3-7 3-7 3-7 3-7 3-7 3-7 3-23 3-48 3-23 3-9 3-48 4-59 3-9 3-11 5-51 5-51 5-51 5-51 3-30 1-2 1-2 3-15 3-15 3-7 3-7 4-39 4-34 3-21 1-8 7-4 3-72 3-7 7-4

5 5 5 5 5 5 5 5 4 4 4 5 5 4 6 4 5 5 5 3 5 4 4 4 4 4 5 4 5 4 4 4 4 4 6 1 6 3 6 6 6 3 3 5 5 4 4 4 5 5 6 6 3 5 2 6 4 5 2 4

35 45 36 39 21 20 20 27 20 23 30 40 36 25 22 16 25 15 21 25 32 4 14 10 4 8 17 10 14 25 20 13 15 17 0 35 22 21 31 13 17 22 22 31 31 16 22 25 39 19 12 11 7 24 12 23 7 16 12 18

2-30 2-30 2-30 2-30 1-5 1-5 1-5 1-5 1-40 1-40 3-11 2-30 2-30 1-39 4-1 4-1 1-39 1-39 1-39 1-5 1-39 4-1 4-1 4-1 4-1 4-1 4-1 2-28 2-28 2-28 2-28 2-28 2-28 1-5 1-40 1-51 1-39 1-39 1-5 1-39 4-1 1-5 1-5 2-14 2-14 4-1 1-39 1-39 3-20 3D-20 2-30 2-30 1-5 3-15 1-5 2-14 1-39 1-47 4-1 2-14

4 4 4 4 3 3 3 3 1 1 2 4 4 2 1 1 1 1 1 1 1 4 4 4 4 4 4 3 3 3 3 3 3 2 3 3 2 1 4 2 1 4 4 2 2 1 2 2 4 4 3 3 2 2 1 1 1 3 1 2

12 30 11 18 20 15 15 20 17 13 35 20 15 13 12 19 33 31 32 20 16 12 11 13 14 13 19 3 7 4 9 1 8 17 18 16 16 19 20 19 13 21 26 28 28 12 24 15 21 17 5 5 8 15 5 14 11 18 5 11

2.B01 Undetermined IgG1 Igκ 4-59 2.B03 Undetermined IgG1 Igκ 3-53 2.C02 Undetermined IgG1 Igλ 4-39 2.G01 Undetermined IgG3 Igκ 7-4-1 2.G03 Undetermined IgG1 Igλ 4-30 CF31 1.B05 CtxA/Holotoxin IgG1 Igκ 3-21 1.D02 CtxA/Holotoxin IgG1 Igκ 3-21 1.E02 CtxB 2 IgG1 Igκ 4-61 1.C02 CtxB 2 IgG1 Igκ 4-61 1.E04 CtxB 3 IgG1 Igκ 5-51 1.C05 CtxB 3 IgG1 Igκ 5-51 1.E06 CtxB IgG1 Igλ 3-30 1.B06 CtxB IgG1 Igλ 3-23 1.F03 CtxB IgG1 Igλ 3-30 1.F05 CtxB IgG1 Igλ 3-43 1.G01 CtxB IgG1 Igλ 3-15 1.G03 CtxB IgG1 Igλ 1-2 1.B04 LPS/OSP 1 IgG1 Igκ 3-23 1.F02 Undetermined 1 IgM Igκ 3-23 1.A01 Undetermined IgG1 Igκ 3-23 1.A03 Undetermined IgG1 Igκ 3-23 1.A04 Undetermined IgG1 Igκ 1-18 1.F04 Undetermined IgG2 Igλ 4-4 1.G02 Undetermined IgG2 Igκ 7-4 1.G05 Undetermined IgG1 Igκ 3-33 * All mAbs were cloned into heavy chain expression vectors containing the IgG1 constant region † FlaA (Flagellin protein), TagA (Lipoprotein ToxR activated gene), PPK (Phosphocarrier protein kinase)

3 4 6 6 6 4 6 2 2 4 4 6 4 6 6 5 4 4 4 2 4 6 3 4 4

23 5 7 13 1 20 20 19 30 27 24 24 20 19 25 14 22 29 27 20 23 28 14 24 24

3-15 3-20 3-1 1-39 1-44 3-20 4-1 1-16 1-16 1D-12 1-12 3-21 3-21 3-21 1D-33 1-40 1-40 3-20 3-20 3-11 1-5 4-1 1-44 4-1 1-5

5 1 1 1 3 2 2 1 1 2 2 1 3 1 5 2 3 4 4 4 1 3 3 1 1

20 6 3 10 1 20 13 15 23 20 18 26 16 28 13 10 8 14 16 8 14 15 13 19 15