Our results show major morphological differences between treated and untreated fibroblasts. Calcein together with CoCl2 appears to be a viable mode; ...
Annals of RSCB
Vol. XV, Issue 2
THE INFLUENCE OF CA2+ IONOPHOR IONOMYCIN ON THE MITOCHONDRIAL TRANSITION PERMEABILITY PORE IN NORMAL AND DRUG TREATED GINGIVAL FIBROBLASTS Ancuta Goriuc, T. Petreus, D. Peptenariu, Roxana Iancu, M. Costuleanu “GR.T. POPA” UNIVERSITY OF MEDICINE AND PHARMACY, IAŞI
Summary Apoptosis represent a form of programmed cell death, characterized by well-defined morphological and biochemical attributes. Until present time, few data regarding Ca2+ involvement in normal gingival fibroblasts apoptosis are available. This paper purpose is to emphasize the effect of ionomycin on normal gingival fibroblasts and also on fibroblasts treated by CsA, nifedipine, phenytoin, by using flow cytometry methods. Gingival fibroblasts were achieved from 6 week old male rats, 150 – 170 g weight, by gingival explants and grown up in specific culture medium consisting of DMEM, 10% fetal bovine serum (FBS), 100 U/ml penicillin, and 100 mg/ml streptomycin in an atmosphere containing 5% CO2 at 37°C. Medium was supplemented with CsA treatment (1µg/ml), nifedipine (3mM) and phenytoin (2,5mM). We have used a control group that received no treatment. Following these steps, flow cytometry using FL1 settings 623 V, 505 V, FL2, 10,000 events, 488 nm laser was performed. Data were processed using FlowJo 7.6.1 software.Statistical data were analyzed by One Way ANOVA method (completed by Student-Newman-Keuls method). Results were considered statistically significant for a p value