Accepted Manuscript The involvement of ginseng berry extract in blood flow via regulation of blood coagulation in rats fed a high-fat diet Min Hee Kim, Jongsung Lee, Sehyun Jung, Joo Wan Kim, Jae-Ho Shin, Hae-Jeung Lee PII:
S1226-8453(16)00006-3
DOI:
10.1016/j.jgr.2016.01.004
Reference:
JGR 164
To appear in:
Journal of Ginseng Research
Received Date: 24 November 2015 Revised Date:
24 January 2016
Accepted Date: 27 January 2016
Please cite this article as: Kim MH, Lee J, Jung S, Kim JW, Shin J-H, Lee H-J, The involvement of ginseng berry extract in blood flow via regulation of blood coagulation in rats fed a high-fat diet, Journal of Ginseng Research (2016), doi: 10.1016/j.jgr.2016.01.004. This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
ACCEPTED MANUSCRIPT 1
The involvement of ginseng berry extract in blood flow via
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regulation of blood coagulation in rats fed a high-fat diet
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RI PT
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Min Hee Kim1),†, Jongsung Lee2),†, Sehyun Jung3), Joo Wan Kim4) , Jae-Ho Shin5), Hae-Jeung
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Lee3)*
SC
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1)
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2)
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University, Gyeonggi, Korea
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3)
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4)
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Republic of Korea
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5)
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†
M AN U
Department of Food and Nutrition, College of Health Industry, Eulji University, Gyeonggi, Korea
TE D
Natural Product Research Center, Aribio Co. ltd. 17 Pangyoro228-gil, Bundang-gu, Sungnam, Gyoenggi-do,
Department of Food and Nutrition, College of Health Industry, Eulji University, Gyeonggi, Korea
These authors contributed equally to this work
AC C
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Department of Genetic Engineering, College of Biotechnology and Bioengineering, Sungkyunkwan
EP
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Department of Physical Therapy, College of Health Science, Eulji University, Gyeonggi, Korea
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*Corresponding author: Hae-Jeung Lee
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Department of Food & Nutrition, Eulji University
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553 Sangseong-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, 461-713, Republic of Korea
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Phone: +82-31-740-7274, Fax: +82-31-740-7370
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E-mail:
[email protected],
[email protected]
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Running title: Effects of ginseng berry extract on blood coagulation 1
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Abstract
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Purpose: The present study investigated the effect of ginseng berry hot water extract (GBx)
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on blood flow via regulation of lipid metabolites and blood coagulation in rats fed a high-fat
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diet (HFD). Methods: Sixty rats were divided into five groups in descending order of body
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weight. Except for the control (CON) group, the other four groups were fed a HFD
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containing 45% kcal from fat for 11 weeks without GBx. GBx groups were then additionally
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treated by gastric gavage with GBx dissolved in distilled water at 50 (GBx 50), 100 (GBx
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100), or 150 (GBx 150) mg/kg body weight for 6 weeks along with the HFD. To investigate
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effects of GBx on rats fed a HFD, biochemical metabolite and blood coagulation assay, and
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histological analysis were performed. Results: In the experiments to measure the serum
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levels of leptin, apolipoprotein (apo) E, and apoB/A, GBx treatment attenuated the HFD-
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induced increases in these metabolites (P
