Downloaded from gut.bmj.com on July 25, 2011 - Published by group.bmj.com
Gut Online First, published on June 14, 2011 as 10.1136/gut.2011.239145 Paper
The putative tumour suppressor microRNA-124 modulates hepatocellular carcinoma cell aggressiveness by repressing ROCK2 and EZH2 Fang Zheng,1,2 Yi-Ji Liao,1,2 Mu-Yan Cai,1,3 Yan-Hui Liu,4 Tian-Hao Liu,1,2,5 Shu-Peng Chen,1,2 Xiu-Wu Bian,6 Xin-Yuan Guan,1,2 Marie C Lin,7 Yi-Xin Zeng,1,2 Hsiang-Fu Kung,1,7 Dan Xie1,2 < Additional materials are
published online only. To view these files please visit the journal online (http://gut.bmj. com). 1
The State Key Laboratory of Oncology in South China, Sun Yat-Sen University, Guangzhou, China 2 Department of Experimental Research, Sun Yat-Sen University, Guangzhou, China 3 Department of pathology, Cancer Center, Sun Yat-Sen University, Guangzhou, China 4 Department of Pathology, Guangdong Provincial People’s Hospital, Guangzhou, China 5 Department of oncology, Sun-Yet-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China 6 Institute of Pathology and Southwest Cancer Center, Southwest Hospital, Third Military Medical University, Chongqing, China 7 The State Key Laboratory of Oncology in South China, the Chinese University of Hong Kong, Hong Kong, China Correspondence to Dr Dan Xie, State Key Laboratory of Oncology in South China, Cancer Center, Sun Yat-Sen University, No. 651, Dongfeng Road East, 510060 Guangzhou, China;
[email protected] Revised 3 May 2011 Accepted 23 May 2011
ABSTRACT Background Recent profile studies of microRNA (miRNA) expression have documented a deregulation of miRNA (miR-124) in hepatocellular carcinoma (HCC). Objective To determine the status of miR-124 expression and its underlying mechanisms in the pathogenesis of HCC. Methods The expression levels of miR-124 were first examined in HCC cell lines and tumour tissues by real-time PCR. The in vitro and in vivo functional effect of miR-124 was examined further. A luciferase reporter assay was conducted to confirm target associations. Results The expression levels of miR-124 were frequently reduced in HCC cells and tissues, and low-level expression of miR-124 was significantly associated with a more aggressive and/or poor prognostic phenotype of patients with HCC (p
