ASSESSMENT OF CARDIO-VENTILATORY AND LOCOMOTOR ACTIVITIES IN THE SHORE CRAB, CARCINUS MAENAS 1)
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A. Robert , T. Monsinjon , N. Elhadji Ille , V. Rasoamampianina , F. Le Foll , 3) 2) 4) 1) F. Durand , J.F. Brethé , J.C. Le Mevel and T. Knigge 1) UMR-I 02 Environmental Stress and aquatic biomonitoring, Le Havre University, France 2) EA 3220 Electrical Engineering and Automatisation Research Group, Le Havre University, France
3) Faculty of Science and Technology, Le Havre University, France 4) LaTIM Laboratory of Neurophysiology, University of Bretagne Occidentale, France
Methods
Background
o Stress free dosing: Injection device* located above the heart
Fluoxetine (FLX) o Antidepressant: Serotonin (5-HT) re-uptake inhibitors
o Data acquisition: Electrodes connected to PowerLab (AD Instruments)
(SSRIs) increase neurotransmission through inhibiting transporter enzymes
A
o 5-HT controls secretion of crustacean hyperglycaemic hormone (CHH)
Haemolymph pressure sensor
o CHH: neuropeptide hormone involved in the mobilisation and release of
Infrared camera-based locomotion tracking
glucose into the haemolymph stimulates cardio-respiratory and locomotor activity
Model organism: the shore crab, Carcinus maenas
o Endpoints:
Cardiovascular activity – Ventilatory activity – Locomotor activity *Robert
o Serotonergic control highly conserved in evolution o Known FLX-related effects on behaviour and locomotion
Cardiovascular activity Electrocardiogram (ECG)
The Y box
et al., Journal of Xenobiotics, 2014
Haemolymph pressure
ECG (μV)
P HEMO (μV)
LED light
Time (sec)
Time (sec)
Haemolymph pressure
o Needle electrodes are placed under the dorsal carapace directly above the heart
ECG
o This position ensures an accurate impedance measure during each heart contraction
A
o A pressure sensor is inserted through the carapace above the heart to detect haemolymph pressure
Ventilatory activity
A
Electroscaphognathitegram (ESG)
ESG (right)
ESG (left)
Locomotor activity Time (sec)
Infrared image
Red blue green image
RIGHT
ESG (μV)
LEFT
Time (sec)
o Specialized appendages termed scaphognathites maintain water current through the gill chamber for oxygen uptake o A single scaphognatite located anterior to the branchial chamber pumps water across the gills by rhythmic beating
A o Development of an infrared camera-based locomotion tracking Xtion Pro Live
o Locomotor activity analysis over a 12h/12h light/dark period and observation of light avoidance reactions
This research was financed by the Interreg IV-A project PeReNE (Peptide Research Network of Excellence)
o Needle electrodes are placed in the gill chamber, which span the scaphognathite and measure its movement through dynamic impedance
Perspectives o Psychoactive drugs affecting serotonergic or dopaminergic control o
A Neurotoxins (e.g. pesticides, methyl-mercury)
o Phycotoxins Contact:
[email protected]