Received: 19 October 2016
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Accepted: 8 May 2017
DOI: 10.1002/mrd.22836
RESEARCH ARTICLE
Transcriptomic profiling reveals disordered regulation of surfactant homeostasis in neonatal cloned bovines with collapsed lungs and respiratory distress Yan Liu1
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Yifan Rao1 | Xiaojing Jiang1,2 | Fanyi Zhang1 | Linhua Huang2 |
Weihua Du1 | Haisheng Hao1 | Xueming Zhao1 | Dong Wang1 | Qiuling Jiang1 | Huabin Zhu1 | Xiuzhu Sun2 1 Institute
of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
2 College
of Animal Science and Technology, Northwest A&F University, Yangling, China
Respiratory distress is a major cause of mortality in cloned neonatal animals, but its pathogenesis remains poorly understood. Here, we used necropsy and histology procedures to evaluate the lungs of cloned neonatal bovines dying of respiratory distress, finding incomplete lung dilation, alveolar collapse, and thickened alveolar
Correspondence Huabin Zhu, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Email:
[email protected] Xiuzhu Sun, College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China. Email:
[email protected] Funding information National Natural Science Foundation of China, Grant number: 31301977; Chinese Academy of Agricultural Sciences Foundation, Grant number: 2017ywf-zd-8; Agricultural Science and Technology Innovation Program, Grant number: ASTIP-IAS06-2017; Chinese Academy of Agricultural Sciences Foundation, Grant number: 2011cj-1
walls. Comparison of the transcriptomes between collapsed lungs of cloned bovines and their normal counterparts revealed 1373 differentially expressed genes in collapsed lungs (p < 0.05, fold change >1.5 or 1.5 or 1.5
(Ikematsu et al., 1993). Briefly, the immunoreactivity score assigns sub-
or
