Two-photon excitation with pico-second fluorescence

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Jan 10, 2012 - for the plant cell studies through an optical parametric oscillator. (OPO ... passed through a 340 ± 20 nm interference filter (U340, Comar ...
Analytica Chimica Acta 719 (2012) 68–75

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Two-photon excitation with pico-second fluorescence lifetime imaging to detect nuclear association of flavanols Irene Mueller-Harvey a,∗ , Walter Feucht b , Juergen Polster c , Lucie Trnková d , Pierre Burgos e , Anthony W. Parker e , Stanley W. Botchway e a Chemistry & Biochemistry Laboratory, Food Production & Quality Research Division, School of Agriculture, Policy & Development, University of Reading, P O Box 236, Reading RG6 6AT, UK b Department of Plant Sciences, Technical University of Munich (TUM), Wissenschaftszentrum Weihenstephan (WZW), D-85354 Freising, Germany c Department of Physical Biochemistry, Technical University of Munich (TUM), Wissenschaftszentrum Weihenstephan (WZW), D-85354 Freising, Germany d University of Hradec Králové, Faculty of Science, Department of Chemistry, Rokitanského 62, 50003 Hradec Králové, Czech Republic e Central Laser Facility, Research Complex at Harwell, Science and Technology Facilities Council, Rutherford Appleton Laboratory, Harwell-Oxford, Didcot, Oxfordshire, OX11 0QX, UK

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Article history: Received 3 November 2011 Received in revised form 21 December 2011 Accepted 23 December 2011 Available online 10 January 2012 Keywords: Fluorescence lifetime imaging microscopy Flavanols Epigallocatechin gallate Nuclear association Histone proteins Multiphoton

a b s t r a c t Two-photon excitation enabled for the first time the observation and measurement of excited state fluorescence lifetimes from three flavanols in solution, which were ∼1.0 ns for catechin and epicatechin, but