Blood smears, hematocrits, and che- motherapy were carried out ..... technical support in animal handling, Jose A. Guevara Patino for optimizing the processing ...
INFECTION AND IMMUNITY, Mar. 2000, p. 1418–1427 0019-9567/00/$04.00⫹0
Vol. 68, No. 3
Vaccine Efficacy of Recombinant Plasmodium falciparum Merozoite Surface Protein 1 in Malaria-Naive, -Exposed, and/or -Rechallenged Aotus vociferans Monkeys ANDREA F. EGAN,1 MICHAEL J. BLACKMAN,2
AND
DAVID C. KASLOW1*
Malaria Vaccines Section and Malaria Vaccine Development Unit, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892,1 and Division of Parasitology, National Institute for Medical Research, London, United Kingdom2 Received 22 June 1999/Returned for modification 18 August 1999/Accepted 2 November 1999
Protection against a lethal challenge infection of Plasmodium falciparum was elicited in malaria-naive Aotus vociferans monkeys by vaccination with the C terminus 19-kDa protein of the major merozoite surface protein (MSP-119) fused to tetanus toxoid universal T-cell epitopes P30 and P2. Three of four monkeys were protected against a 104-parasite challenge. Four monkeys were challenged with 105 parasites; one self-cured the infection, two were protected against high parasitemia (
