Department of Biology, University of North Carolina at Charlotte, Charlotte, North Carolina 28223 ..... were sometimes inconsistent, as different batches of oysters.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, Apr. 1996, p. 1378–1382 0099-2240/96/$04.0010 Copyright q 1996, American Society for Microbiology
Vol. 62, No. 4
Detection of Vibrio vulnificus Biotypes 1 and 2 in Eels and Oysters by PCR Amplification SHIRLEY S. COLEMAN,1 DENISE M. MELANSON,1† ELENA G. BIOSCA,2
AND
JAMES D. OLIVER1*
Department of Biology, University of North Carolina at Charlotte, Charlotte, North Carolina 28223,1 and Departamento de Microbiologia, Universidad de Valencia, Burjassot E-46100, Valencia, Spain2 Received 17 November 1995/Accepted 9 February 1996
DNA extraction procedures and PCR conditions to detect Vibrio vulnificus cells naturally occurring in oysters were developed. In addition, PCR amplification of V. vulnificus from oysters seeded with biotype 1 cells was demonstrated. By the methods described, V. vulnificus cells on a medium (colistin-polymyxin B-cellobiose agar) selective for this pathogen were detectable in oysters harvested in January and March, containing no culturable cells (
