Objective: To examine the effect of CSD on the distribution of c-Fos ... using anti-c-Fos antibody in coronal slices of whole brain isolated after CSD elicitation.
Abstracts / Journal of the Neurological Sciences (2017) 945–1128
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alterations, seizure, or decreased level of consciousness. Encephalitic features developed rapidly within median 5 days (range, 1-26 days). Fever was more frequently seen in patients with headache than those without (10/19 vs 1/15, p= 0.008). Only one of the 19 patients underwent lumbar puncture before the onset of encephalitic features but in the others headache was regarded as cold. The median white blood cells in CSF were higher in patients with headache than those without (61/μL vs 25/μL, p=0.010), but CSF protein level, or frequency of CSF oligoclonal bands, MRI abnormalities or tumorassociation, was not different between two groups. Conclusion: It remains unclear whether headache is caused by infection or autoimmune mechanism; however, 56% of patients reported headache initially. Two-week observation for encephalitic features is important especially in young woman with new-onset of headache and/or fever. doi:10.1016/j.jns.2017.08.2665
2634 WCN17-3293 SHIFT 7 - HEADACHE Widespread c-Fos expression after cortical spreading depression; possible differences between two types of animal models of familial hemiplegic migraine 2 M. Unekawaa, K. Ikedab,c, Y. Tomitaa,d, K. Kawakamic, N. Suzukia. aKeio University School of Medicine, Neurology, Tokyo, Japan; bInternational University of Health and Welfare- School of Medicine, Physiology, Narita, Japan; cJichi Medical School, Center for Molecular MedicineBiology, Shimotsuke, Japan; dTomita Hospital, Neurology, Okazaki, Japan Background: Migraine aura may be related to cortical spreading depression (CSD), which involves mass depolarization of neurons and astrocytes that propagates through gray matter of cerebral cortex. Atp1a2-knockout mice are models of familial hemiplegic migraine type 2 (FHM2), and exhibit a low threshold to CSD elicitation. Objective: To examine the effect of CSD on the distribution of c-Fos expression, which reflects neural activation, in two types of Atp1a2knockout mice, Atp1a2tm1Kwk (C-KO) and Atp1a2tm2Kwk (N-KO). Patients and Methods / Material and Methods: Five or 6 CSDs were elicited unilaterally with KCl in C-KO and N-KO heterozygous mice and wild-type littermates under urethane anesthesia with mechanical ventilation (n=20). Wild-type mice were used for sham-operation and electroencephalographic (EEG) analysis (n=5 and 20, respectively). Enhanced immunohistochemistry was performed using anti-c-Fos antibody in coronal slices of whole brain isolated after CSD elicitation.
c-Fos-positive cells were counted in specified regions and compared with those in contralateral regions. Results: We found c-Fos-positive cells in most regions of the ipsilateral cortices after CSD, especially in somatosensory cortex, basal ganglia (striatum) and limbic system (piriform cortex and the amygdala). Bilateral enhancement was observed in spinal trigeminal nucleus caudalis (Sp5C) and there was no difference in septal nucleus. Immunoreactivity was significantly suppressed in the contralateral somatosensory cortex, concomitantly with mild EEG suppression. c-Fos expression tended to be higher in C-KO and N-KO heterozygotes than in wild-type. Conclusion: Our results may suggest that enhanced c-Fos expression in some brain regions of heterozygotes may reflect their higher sensitivity in eliciting CSD compared with wild-type. doi:10.1016/j.jns.2017.08.2666
2635 WCN17-0990 SHIFT 7 - HEADACHE Migraine-related ischemic stroke Y. Unnoa, K. Nagaib, T. Johnoc, R. Hiranoa. aKyorin University, Department of Stroke and Cerebrovascular Medicine, Tokyo, Japan; b Kyorin University Hospital, Neurology, Tokyo, Japan; cKyorin University Hospital, Stroke and Cerebrovascular Medicine, Tokyo, Japan Background: The association between migraine and stroke still remain unclear. Migraine with aura (MA) has been described to be associated with an increased risk for ischemic stroke. Most studies have shown a lack of association between migraine without aura (MO) and ischemic stroke. According to the International Classification of Headache Disorders, 3rd Edition beta version (ICHD-3beta), migrainous infarction is rare. On the other hand, migraine is associated with an increased risk of silent brain lesions detected by magnetic resonance imaging. Objective: Purpose of this study is to evaluate clinical characteristics of migraine-related stroke. Patients and Methods / Material and Methods: Patients who admitted to our Stroke Unit and had been diagnosed as having ischemic stroke which developed during migraine attack were recruited. Results: A 56-year-old female, with a history of MA and MO, developed dysarthria, left-sided weakness and numbness during her typical migraine attack without aura. Diffusion-weighted image of
