Innovare
International Journal of Pharmacy and Pharmaceutical Sciences
Academic Sciences
ISSN- 0975-1491
Vol 7, Issue 3, 2015
Original Article
XANTHINE OXIDASE INHIBITORY AND ANTIHYPERURICEMIC ACTIVITIES OF ANREDERA CORDIFOLIA (TEN) STEENIS, SONCHUS ARVENSIS L, AND ITS COMBINATION KURNIA DWI WIDYARINIab, ELIN YULINAH SUKANDARa, IRDA FIDRIANNYa aSchool
of Pharmacy, Bandung Institute of Technology, Jalan Ganeca No. 10, Bandung, Indonesia, bNational Agency of Drug and Food Control, Jalan Percetakan Negara No. 23, Jakarta, Indonesia. Email:
[email protected] Received: 10 Dec 2014 Revised and Accepted: 31 Dec 2014
ABSTRACT Objective: The objective of this research was to evaluate the xanthine oxidase (XO) inhibitory activity and the ability to reduce serum uric acid levels of 70% ethanol extracts of Anredera cordifolia (ACE) and Sonchus arvensis (SAE) leaves, and its combinations. Methods: Inhibitory activity of XO was determined by measuring uric acid formation by UV spectrophotometry. Moreover, the antihyperuricemic assay conducted in vivo in male Wistar rats was induced by high-purine diet and potassium oxonate (PO). The reduction of serum uric acid levels after extracts administration was observed and compared to allopurinol.
Results: The IC 50 results of ACE, SAE, and the combination of both with a ratio of 1:1 were obtained 635.25 ppm, 1345.93 ppm, and 846.32 ppm, respectively. The IC 50 of allopurinol as reference was 0.88 ppm. Hence, XO inhibitory activity of the combination extracts was additive. Results of antihyperuricemic assay showed that uric acid levels of the group of allopurinol, ACE, SAE, and the combination of both extracts were significantly lower compared to the positive control group at 120 and 150 minutes after PO induction (p
